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Full-Text Articles in Biomedical Engineering and Bioengineering
High-Throughput Metabolism-Induced Toxicity Assays On A 384-Pillar Plate, Sooyeion Kang
High-Throughput Metabolism-Induced Toxicity Assays On A 384-Pillar Plate, Sooyeion Kang
ETD Archive
The U.S Environmental Protection Agency (EPA) launched the Transform Tox Testing Challenge in 2016 with the goal of developing practical methods that can be integrated into conventional high-throughput screening (HTS) assays to better predict the toxicity of parent compounds and their metabolites in vivo. In response to this need and to retrofit existing HTS assays for assessing metabolism-induced toxicity of compounds, we have developed a 384-pillar plate that is complementary to traditional 384-well plates and ideally suited for culturing human cells in three dimensions (3D) at a microscale. Briefly, human embryonic kidney (HEK) 293 cells in a mixture of alginate …
Modeling Liver Diseases Using Hepatic Cell Microarrays, Alexander David Roth
Modeling Liver Diseases Using Hepatic Cell Microarrays, Alexander David Roth
ETD Archive
Hepatocellular carcinoma (HCC) is an invasive and aggressive cancer of the liver that arises due to chronic cirrhosis. Research into understanding HCC has focused on two-dimensional (2D) and three-dimensional (3D) technologies to simulate the liver microenvironment and use animal models to model how HCC affects the rest of the body. 3D hydrogel models are desired because they can mimic the transport behavior observed in vivo by structurally mimicking the extracellular matrix (ECM) without the ethical concerns of animal models. However, hydrogels can be toxic to cells and require optimal procedures for appropriate handling. In this study, we created 3D models …