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Design Of Recombinant Tenebrio Molitor Antifreeze Protein For Purification Using Elastin-Like Polypeptide Tag, Joongseok Oh

ETD Archive

Fusion protein technologies can aid to improve solubility of recombinant protein from microorganisms and help recombinant protein purification. Elastin-like polypeptides (ELP) as a fusion tag can be utilized to facilitate the purification of recombinant proteins because ELP can provide its thermally responsive behavior to ELP tagged proteins. An ELP tag can be used as the purification carrier through inverse transition cycling (ITC), which is a simple and non chromatographic separation process. The purification through ITC can reduce cost and can be quickly performed compared to other purification methods. However, we further considered ELP tag removal because it may possibly hinder …