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Full-Text Articles in Biomedical Engineering and Bioengineering
Cell Express 100tm - A Robust, Simple And Cost Effective Alternative To Highthroughput Automated Platforms For Cell Line Development, Raj Kumar Kunaparaju
Cell Express 100tm - A Robust, Simple And Cost Effective Alternative To Highthroughput Automated Platforms For Cell Line Development, Raj Kumar Kunaparaju
Cell Culture Engineering XV
The unrivalled growth in monoclonal antibody (mAb) therapeutics is demanding for a rapid, simple and cost effective expression system. Conventional expression system makes use of Gene Amplification Strategies, Chromatin Modifying Elements and High-Throughput Automated Platforms for cell line development. All these process are complex and costly. CELL EXPRESS 100TM with its simple selection procedure and being able to generate 100% stable high expressing pool simplifies the isolation of high producing cell line without the need for Amplification, Chromatin Modifying Elements and High Throughput Automated Platforms. CELL EXPRESS 100TM expression system consists of an expression vector (pUB-CE-100) and a …
On The Orders Of Magnitude Of Epigenic Dynamics And Monoclonal Antibody Production, Joanne M. Savinell (Belovich), Gyun Min Lee, Bernhard O. Palsson
On The Orders Of Magnitude Of Epigenic Dynamics And Monoclonal Antibody Production, Joanne M. Savinell (Belovich), Gyun Min Lee, Bernhard O. Palsson
Chemical & Biomedical Engineering Faculty Publications
The hybridoma cell's maximum capacity for monoclonal antibody (MAb) production is estimated to be 2300–8000 MAb molecules/cell/s, using measured rates of transcription and translation, and the limitations imposed by the size of the polymerase molecule and the ribosome. Nearly all the production rates reported in the literature fall into or below this range of production rates. Data from batch cultures of hybridomas demonstrate a constant specific rate of MAb production until the time integral of the viable cell concentration reaches about 108 cells · h/cm3. At this point, some essential nutrients from the standard media …