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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Simulation Of Free Radical Reactions In Biology And Medicine: A New Two-Compartment Kinetic Model Of Intracellular Lipid Peroxidation, Charles F. Babbs, Melissa Gale Steiner
Simulation Of Free Radical Reactions In Biology And Medicine: A New Two-Compartment Kinetic Model Of Intracellular Lipid Peroxidation, Charles F. Babbs, Melissa Gale Steiner
Weldon School of Biomedical Engineering Faculty Publications
To explore mechanisms of free radical reactions leading to intracellular lipid peroxidation in living systems, we developed a computational model of up to 109 simultaneous enzymatic and free radical reactions thought to be involved in the initiation, propagation, and termination of membrane lipid peroxidation. Rate constants for the various reactions were obtained from the published literature. The simulation model included a lipid membrane compartment and an aqueous cytosolic compartment, between which various chemical species were partitioned. Lipid peroxidation was initiated by the iron-catalyzed, superoxide-driven Fenton reaction. A "C"-language computer program implemented numerical solution of the steady-state rate equations for concentrations …
Scatchard Analysis Of Methane Sulfinic Acid Production From Dimethyl Sulfoxide: A Method To Quantify Hydroxyl Radical Formation In Physiologic Systems, Charles F. Babbs, David W. Griffin
Scatchard Analysis Of Methane Sulfinic Acid Production From Dimethyl Sulfoxide: A Method To Quantify Hydroxyl Radical Formation In Physiologic Systems, Charles F. Babbs, David W. Griffin
Weldon School of Biomedical Engineering Faculty Publications
A major impediment to the confirmation of free radical mechanisms in pathogenesis is a lack of direct, chemical evidence that oxygen centered free radicals actually arise in living tissues in quantities sufficient to cause serious damage. This investigation was conducted to validate the use of dimethyl sulfoxide (DMSO) as a quantitative molecular probe for the generation of hydroxyl radicals (HO*) under physiologic conditions. Reaction of HO* with DMSO produces methane sulfinic acid (MSA) as a primary product, which can be detected by a simple colorimetric assay. To develop a method for estimating total HO* production, we studied two model systems: …
Protection From Reperfusion Injury In The Isolated Rat Heart By Postischaemic Deferoxamine And Osypurinol Administration, Stephen F. Badylak, Abby Simmons, John Turek, Charles F. Babbs
Protection From Reperfusion Injury In The Isolated Rat Heart By Postischaemic Deferoxamine And Osypurinol Administration, Stephen F. Badylak, Abby Simmons, John Turek, Charles F. Babbs
Weldon School of Biomedical Engineering Faculty Publications
The Langendorff isolated rat heart preparation was used to determine the effect of oxypurinol, a xanthine oxidase inhibitor, and deferoxamine, an iron binding agent, on the extent of myocardial reperfusion injury after 60 minutes of ischaemia. Thirty rats were divided into three groups of 10. and an isolated heart preparation made from each rat. The isolated hearts were perfused for 15 minutes with a modified Krebs-Henseleit perfusate solution to permit stabilisation of the preparation. Each heart was then subjected to 60 minutes of total ischaemia at 37°C followed by 60 minutes of reperfusion with either saline treated perfusate, oxypurinol treated …