Open Access. Powered by Scholars. Published by Universities.®
Biomedical Engineering and Bioengineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Biomedical Engineering and Bioengineering
Unstable Spinocerebellar Ataxia Type 10 (Attct) (Agaat) Repeats Are Associated With Aberrant Replication At The Atx10 Locus And Replication Origin-Dependent Expansion At An Ectopic Site In Human Cells, Guoqi Liu, John J. Bissler, Richard R. Sinden, Michael Leffak
Unstable Spinocerebellar Ataxia Type 10 (Attct) (Agaat) Repeats Are Associated With Aberrant Replication At The Atx10 Locus And Replication Origin-Dependent Expansion At An Ectopic Site In Human Cells, Guoqi Liu, John J. Bissler, Richard R. Sinden, Michael Leffak
Biomedical Engineering and Sciences Faculty Publications
Spinocerebellar ataxia type 10 (SCA10) is associated with expansion of (ATTCT)n repeats (where n is the number of repeats) within the ataxin 10 (ATX10/E46L) gene. The demonstration that (ATTCT)n tracts can act as DNA unwinding elements (DUEs) in vitro has suggested that aberrant replication origin activity occurs at expanded (ATTCT)n tracts and may lead to their instability. Here, we confirm these predictions. The wild-type ATX10 locus displays inefficient origin activity, but origin activity is elevated at the expanded ATX10 loci in patient-derived cells. To test whether (ATTCT)n tracts can potentiate origin activity, cell lines were constructed that contain ectopic copies …