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Biomedical Engineering and Bioengineering Commons™
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Articles 1 - 6 of 6
Full-Text Articles in Biomedical Engineering and Bioengineering
Overexpression Of Timp-3 In Chondrocytes Produces Transient Reduction In Growth Plate Length But Permanently Reduces Adult Bone Quality And Quantity, Blandine Poulet, Ke Liu, Darren A. Plumb, Phoung Vo, Mittal Shah, Katherine Ann Staines, Alexandra Sampson, Hiroyuke Nakamura, Hideaki Nagase, Alessandra Carriero, Sandra J. Shefelbine, Andrew A. Pitsillides, George Bou-Gharios
Overexpression Of Timp-3 In Chondrocytes Produces Transient Reduction In Growth Plate Length But Permanently Reduces Adult Bone Quality And Quantity, Blandine Poulet, Ke Liu, Darren A. Plumb, Phoung Vo, Mittal Shah, Katherine Ann Staines, Alexandra Sampson, Hiroyuke Nakamura, Hideaki Nagase, Alessandra Carriero, Sandra J. Shefelbine, Andrew A. Pitsillides, George Bou-Gharios
Biomedical Engineering and Sciences Faculty Publications
Bone development and length relies on the growth plate formation, which is dependent on degradative enzymes such as MMPs. Indeed, deletion of specific members of this enzyme family in mice results in important joint and bone abnormalities, suggesting a role in skeletal development. As such, the control of MMP activity is vital in the complex process of bone formation and growth. We generated a transgenic mouse line to overexpress TIMP3 in mouse chondrocytes using the Col2a1-chondrocyte promoter. This overexpression in cartilage resulted in a transient shortening of growth plate in homozygote mice but bone length was restored at eight weeks …
The Quantum Biology Of Reactive Oxygen Species Partitioning Impacts Cellular Bioenergetics, Robert J. Usselman, Cristina Chavarriaga, Pablo R. Castello, Maria Procopio, Thorsten Ritz, Edward A. Dratz, David John Singel, Carlos F. Martino
The Quantum Biology Of Reactive Oxygen Species Partitioning Impacts Cellular Bioenergetics, Robert J. Usselman, Cristina Chavarriaga, Pablo R. Castello, Maria Procopio, Thorsten Ritz, Edward A. Dratz, David John Singel, Carlos F. Martino
Biomedical Engineering and Sciences Faculty Publications
Quantum biology is the study of quantum effects on biochemical mechanisms and biological function. We show that the biological production of reactive oxygen species (ROS) in live cells can be influenced by coherent electron spin dynamics, providing a new example of quantum biology in cellular regulation. ROS partitioning appears to be mediated during the activation of molecular oxygen (O2) by reduced flavoenzymes, forming spin-correlated radical pairs (RPs). We find that oscillating magnetic fields at Zeeman resonance alter relative yields of cellular superoxide (O2•−) and hydrogen peroxide (H2O2) ROS products, indicating coherent singlet-triplet mixing at the point of ROS formation. Furthermore, …
Tunneling Nanotubes: An Alternate Route For Propagation Of The Bystander Effect Following Oncolytic Viral Infection, Justin Ady, Venugopal Thayanithy, Kelly Mojica, Phillip Wong, Joshua Carson, Prassanna Rao, Yuman Fong, Emil Lou
Tunneling Nanotubes: An Alternate Route For Propagation Of The Bystander Effect Following Oncolytic Viral Infection, Justin Ady, Venugopal Thayanithy, Kelly Mojica, Phillip Wong, Joshua Carson, Prassanna Rao, Yuman Fong, Emil Lou
Biomedical Engineering and Sciences Faculty Publications
Tunneling nanotubes (TNTs) are ultrafine, filamentous actin-based cytoplasmic extensions which form spontaneously to connect cells at short and long-range distances. We have previously described long-range intercellular communication via TNTs connecting mesothelioma cells in vitro and demonstrated TNTs in intact tumors from patients with mesothelioma. Here, we investigate the ability of TNTs to mediate a viral thymidine kinase based bystander effect after oncolytic viral infection and administration of the nucleoside analog ganciclovir. Using confocal microscopy we assessed the ability of TNTs to propagate enhanced green fluorescent protein (eGFP), which is encoded by the herpes simplex virus NV1066, from infected to uninfected …
Impaired Corpus Cavernosum Relaxation Is Accompanied By Increased Oxidative Stress And Up-Regulation Of The Rho-Kinase Pathway In Diabetic (Db/Db) Mice, Fernanda Bruschi Marinho Priviero, Haroldo A.F. Toque, Kênia Pedrosa Nunes, Denise Gonçalves Priolli, Cleber Evandro Teixeira, Robert Clinton Webb
Impaired Corpus Cavernosum Relaxation Is Accompanied By Increased Oxidative Stress And Up-Regulation Of The Rho-Kinase Pathway In Diabetic (Db/Db) Mice, Fernanda Bruschi Marinho Priviero, Haroldo A.F. Toque, Kênia Pedrosa Nunes, Denise Gonçalves Priolli, Cleber Evandro Teixeira, Robert Clinton Webb
Biomedical Engineering and Sciences Faculty Publications
Basal release of nitric oxide from endothelial cells modulates contractile activity in the corpus cavernosum via inhibition of the RhoA/Rho-kinase signaling pathway. We aimed to investigate nitric oxide bioavailability, oxidative stress and the Rho-kinase pathway in the relaxation of the corpus cavernosum of an obese and diabetic model of mice (db/db mice). We hypothesized that in db/db mice impaired relaxation induced by Rho-kinase inhibitor is accompanied by diminished NO bioavailability, increased oxidative stress and upregulation of the RhoA/Rho-kinase signalling pathway. Cavernosal strips from male lean and non-diabetic db/+ and db/db mice were mounted in myographs and isometric force in response …
Impact Of Elastin Incorporation Into Electrochemically Aligned Collagen Fibers On Mechanical Properties And Smooth Muscle Cell Phenotype, Thuy-Uyen Nguyen, Chris A. Bashur, Vipuil Kishore
Impact Of Elastin Incorporation Into Electrochemically Aligned Collagen Fibers On Mechanical Properties And Smooth Muscle Cell Phenotype, Thuy-Uyen Nguyen, Chris A. Bashur, Vipuil Kishore
Biomedical Engineering and Sciences Faculty Publications
Application of tissue-engineered vascular grafts (TEVGs) for the replacement of small-diameter arteries is limited due to thrombosis and intimal hyperplasia. Previous studies have attempted to address the limitations of TEVGs by developing scaffolds that mimic the composition (collagen and elastin) of native arteries to better match the mechanical properties of the graft with the native tissue. However, most existing scaffolds do not recapitulate the aligned topography of the collagen fibers found in native vessels. In the current study, based on the principles of isoelectric focusing, two different types of elastin (soluble and insoluble) were incorporated into highly oriented electrochemically aligned …
Animal Models Of Osteogenesis Imperfecta: Applications In Clinical Research, Tanya A. Enderli, Stephanie R. Burtch, Jara N. Templet, Alessandra Carriero
Animal Models Of Osteogenesis Imperfecta: Applications In Clinical Research, Tanya A. Enderli, Stephanie R. Burtch, Jara N. Templet, Alessandra Carriero
Biomedical Engineering and Sciences Faculty Publications
Osteogenesis imperfecta (OI), commonly known as brittle bone disease, is a genetic disease characterized by extreme bone fragility and consequent skeletal deformities. This connective tissue disorder is caused by mutations in the quality and quantity of the collagen that in turn affect the overall mechanical integrity of the bone, increasing its vulnerability to fracture. Animal models of the disease have played a critical role in the understanding of the pathology and causes of OI and in the investigation of a broad range of clinical therapies for the disease. Currently, at least 20 animal models have been officially recognized to represent …