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Full-Text Articles in Biomedical Engineering and Bioengineering
Ranbp9 Aggravates Synaptic Damage In The Mouse Brain And Is Inversely Correlated To Spinophilin Levels In Alzheimer's Brain Synaptosomes, Juan Pablo Palavicini, Hongjie Wang, Elisabetta Bianchi, Shaohua Xu, Jagadeesh S. Rao, David E. Kang, Madepalli K. Lakshmana
Ranbp9 Aggravates Synaptic Damage In The Mouse Brain And Is Inversely Correlated To Spinophilin Levels In Alzheimer's Brain Synaptosomes, Juan Pablo Palavicini, Hongjie Wang, Elisabetta Bianchi, Shaohua Xu, Jagadeesh S. Rao, David E. Kang, Madepalli K. Lakshmana
Biomedical Engineering and Sciences Faculty Publications
We previously demonstrated that over expression of RanBP9 led to enhanced Ab generation in a variety of cell lines and primary neuronal cultures, and subsequently, we confirmed increased amyloid plaque burden in a mouse model of Alzheimer’s disease (AD). In the present study, we found striking reduction of spinophilin protein levels when RanBP9 is overexpressed. At 12 months of age, we found spinophilin levels reduced by 70% (Po0.001) in the cortex of APDE9/RanBP9 mice compared with that in wild-type (WT) controls. In the hippocampus, the spinophilin levels were reduced by 45% (Po0.01) in the APDE9/RanBP9 mice. Spinophilin immunoreactivity was also …
Techniques To Quantify The Size Of Protein Colloids In Amyloid Fiber Formation, Jonathan R. Anson, Chia-Hung Lu, Lingwen Cui, Xiaojing Yang, Shaohua Xu
Techniques To Quantify The Size Of Protein Colloids In Amyloid Fiber Formation, Jonathan R. Anson, Chia-Hung Lu, Lingwen Cui, Xiaojing Yang, Shaohua Xu
Biomedical Engineering and Sciences Faculty Publications
A new method for the analysis of protein colloidal diameter has been developed using three existing protein concentration quantification techniques, absorption at 280 nm, colloidal gold assay, and DC protein assay. Protein colloids are formed in the process of aggregation and are thought to be intermediates in protein self-assembly and formation of amyloid fiber. Deposition of the protein fibers in tissues leads to numerous human diseases including Alzheimer’s. Lysozyme was incubated at pH 2.0, 55°C, an environment conducive to amyloid fiber formation. The protein colloids present in the supernatant of the samples after centrifugation were studied over a time course …