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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Compact Dual Wedge Point Scanning Confocal Reflectance Microscope, William C. Warger Ii, Stephen A. Guerrera, Charles A. Dimarzio
Compact Dual Wedge Point Scanning Confocal Reflectance Microscope, William C. Warger Ii, Stephen A. Guerrera, Charles A. Dimarzio
Charles A. DiMarzio
Confocal reflectance microscopy has been shown to provide optical sectioning and resolution sufficient to provide useful information about skin to a depth below the epidermis. However, existing instruments are large and expensive, because of the need for fast twodimensional scanning in the pupil, and the associated relay optics. A more compact scanning system could lead to an affordable hand-held instrument for in vivo imaging. Several approaches are being considered with different advantages and disadvantages. Here we report one approach that incorporates a dual-wedge scanner within a point-scanning configuration. The dual-wedge scanner is implemented by replacing the two scanning mirrors and …
Dna− Gold Nanoparticle Reversible Networks Grown On Cell Surface Marker Sites: Application In Diagnostics, Kyuwan Lee
Dna− Gold Nanoparticle Reversible Networks Grown On Cell Surface Marker Sites: Application In Diagnostics, Kyuwan Lee
Kyuwan Lee
Effective identification of breast cancer stem cells (CSC) benefits from a multiplexed approach to detect cell surface markers that can distinguish this subpopulation, which can invade and proliferate at sites of metastasis. We present a new approach for dual-mode sensing based on targeting using pointer and signal enhancement using enhancer particle networks for detection by surface plasmon resonance (SPR) and surface-enhanced Raman scattering (SERS). We demonstrate our concept to detect cell surface markers, CD44 and CD24, in three breast cancer cell lines to identify a CD44+/CD24− subpopulation of CSCs. The designed network structure can be well-controlled and has improved sensitivity …