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Biomedical Engineering and Bioengineering Commons™
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- Adipic acid (1)
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- Rifampicin; drug delivery; encapsulation; submicron particles; poly-L-lactide; L-lactide grafted glucosamine; tuberculosis; intracellular killing (1)
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- Thrombomodulin (1)
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Articles 1 - 5 of 5
Full-Text Articles in Biomedical Engineering and Bioengineering
Inducing Increased Bioplastic Production In R. Palustris Cga009, Cameron Gilley, Dylan Hoppner, Brandi J. Brown
Inducing Increased Bioplastic Production In R. Palustris Cga009, Cameron Gilley, Dylan Hoppner, Brandi J. Brown
Department of Chemical and Biomolecular Engineering: Theses and Student Research
PHA’s (polyhydroxyalkanoates) are important bio polymers in different industries such as petroleum, medicine, and nano technology. In the microorganisms in which they are produced, they serve as an energy storage material by storing both carbon and usable electrons. This is useful in environments where the organisms are nutrient starved. PHA’s have a practical use especially in the medical field as bio-plastics because they are biodegradable and bio-compatible. Rhodopseudomonas. palustris, a common soil bacterium, is notable for its uncommon metabolic flexibility. Its diverse metabolism means that it can fix CO 2 and grow on many lignin based monomers in both aerobic …
Bioproduction Of Adipic Acid Using Engineered Pseudomonas Putida Kt2440 From Lignin-Derived Aromatics, Howard Willett
Bioproduction Of Adipic Acid Using Engineered Pseudomonas Putida Kt2440 From Lignin-Derived Aromatics, Howard Willett
Department of Chemical and Biomolecular Engineering: Theses and Student Research
Current industrial synthesis of adipic acid is nonrenewable and depends on a carcinogenic starting material, benzene. Biocatalysis with an engineered microorganism could turn a renewable feedstock into a value-added chemical such as adipic acid. Here we engineered P. putida KT2440 to transform lignin-derived aromatics, coumarate and ferulate, into adipic acid. Lignin is a recalcitrant plant biopolymer burned for thermal energy. Conversion of lignin into a value-added chemical will improve the efficiency of lignocellulose processing plants. The best performing engineered KT2440 strain produces 2.52 mM adipate at a 9.5% (mole/mole) yield. This was achieved by the genetic insertion of non-natural biosynthetic …
Fibrinogen, Factor Xiii And Fibronectin: A Biophysical And Kinetic Characterization Of Their Interactions, Frank Fabian
Fibrinogen, Factor Xiii And Fibronectin: A Biophysical And Kinetic Characterization Of Their Interactions, Frank Fabian
Department of Chemical and Biomolecular Engineering: Theses and Student Research
The development of recombinant-based liquid fibrin tissue sealants having enhanced hemostatic and wound healing properties will involve understanding as yet not well characterized interactions between fibrinogen, fibrin (Fbn) factor XIII, thrombin and fibronectin. We study these phenomena in the context of comparing plasma derived fibrinogen to recombinant fibrinogen (rFI) produced in the milk of transgenic cows. An abundance of purified γγ and γγ’ FI subspecies enables detailed study of γγ or γγ’ biomonomer and their respective Fbn biopolymer formation as having different substrate behaviors of activated plasma derived factor XIII (pFXIIIa2b2). High pressure size exclusion (HPSEC) …
Low Molecular Weight Glucosamine/L-Lactide Copolymers As Potential Carriers For The Development Of A Sustained Rifampicin Release System: Mycobacterium Smegmatis As A Tuberculosis Model, Jorge Ragusa
Department of Chemical and Biomolecular Engineering: Theses and Student Research
Tuberculosis, a highly contagious disease, ranks as the second leading cause of death from an infectious disease, and remains a major global health problem. In 2013, 9 million new cases were diagnosed and 1.5 million people died worldwide from tuberculosis. This dissertation aims at developing a new, ultrafine particle-based efficient antibiotic delivery system for the treatment of tuberculosis. The carrier material to make the rifampicin (RIF)-loaded particles is a low molecular weight star-shaped polymer produced from glucosamine (molecular core building unit) and L-lactide (GluN-LLA). Stable particles with a very high 50% drug loading capacity were made via electrohydrodynamic atomization. Prolonged …
Synthesis Of An Endothelial Cell Mimicking Surface Containing Thrombomodulin And Endothelial Protein C Receptor, Karl E. Kador
Synthesis Of An Endothelial Cell Mimicking Surface Containing Thrombomodulin And Endothelial Protein C Receptor, Karl E. Kador
Department of Chemical and Biomolecular Engineering: Theses and Student Research
Synthetic materials for use in blood contacting applications have been studied for many years with limited success. One of the main areas of need for these materials is the design of synthetic vascular grafts for use in the hundreds of thousands of patients who have coronary artery bypass grafting, many without suitable veins for autologous grafts. The design of these grafts is constrained by two common modes of failure, the formation of intimal hyperplasia (IH) and thrombosis. IH formation has been previously linked to a mismatching of the mechanical properties of the graft and has been overcome by creating grafts …