Biomedical Engineering and Bioengineering Commons^™

Apparatus For Illuminating The Tip-Sample Interface Of An Atomic Force Microscope, Nancy Burnham, E Thoreson

Nancy A. Burnham

An apparatus for the delivery of radiation to the tip-sample interface of an atomic force microscope(AFM) is demonstrated. The pulsed light delivery system (PLDS) was fabricated to probe photoinduced conformational changes of molecules using an AFM. The system is 67 mm long, 59 mm wide, and 21 mm high, leaving clearance to mount the PLDS and a microscope slide coated with a thin film of photoactive molecules beneath the cantilever tip of a stand-alone AFM. The PLDS is coupled into a fiber pigtailed Nd:yttrium–aluminum–garnetfrequency doubled laser, operating at a wavelength of 532 nm. The radiation delivered to a sample through …