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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Photolithographic Surface Micromachining Of Polydimethylsiloxane (Pdms), Weiqiang Chen, Raymond H. W. Lam, Jianping Fu
Photolithographic Surface Micromachining Of Polydimethylsiloxane (Pdms), Weiqiang Chen, Raymond H. W. Lam, Jianping Fu
Weiqiang Chen
A major technical hurdle in microfluidics is the difficulty in achieving high fidelity lithographic patterning on polydimethylsiloxane (PDMS). Here, we report a simple yet highly precise and repeatable PDMS surface micromachining method using direct photolithography followed by reactive ion etching (RIE). Our method to achieve surface patterning of PDMS applied an O2 plasma treatment to PDMS to activate its surface to overcome the challenge of poor photoresist adhesion on PDMS for photolithography. Our photolithographic PDMS surface micromachining technique is compatible with conventional soft lithography techniques and other silicon-based surface and bulk micromachining methods. To illustrate the general application of our …
Dna− Gold Nanoparticle Reversible Networks Grown On Cell Surface Marker Sites: Application In Diagnostics, Kyuwan Lee
Dna− Gold Nanoparticle Reversible Networks Grown On Cell Surface Marker Sites: Application In Diagnostics, Kyuwan Lee
Kyuwan Lee
Effective identification of breast cancer stem cells (CSC) benefits from a multiplexed approach to detect cell surface markers that can distinguish this subpopulation, which can invade and proliferate at sites of metastasis. We present a new approach for dual-mode sensing based on targeting using pointer and signal enhancement using enhancer particle networks for detection by surface plasmon resonance (SPR) and surface-enhanced Raman scattering (SERS). We demonstrate our concept to detect cell surface markers, CD44 and CD24, in three breast cancer cell lines to identify a CD44+/CD24− subpopulation of CSCs. The designed network structure can be well-controlled and has improved sensitivity …