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Biomedical Engineering and Bioengineering Commons™
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- Angiogenesis (2)
- Arteriogenesis (2)
- Mouse (2)
- Peripheral Artery Disease (2)
- Transfemoral (2)
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- Abdominal exercise (1)
- Age (1)
- Amphiphilic Polymer Wrapping (1)
- Amputee (1)
- Amyloid (1)
- Aqueous Soluble Quantum Dot (1)
- Bag (1)
- Biological Imaging (1)
- Biomarker (1)
- Biomarkers (1)
- Bionanotechnology (1)
- Biosensors (1)
- Calibrated Cuff Plethysmography (1)
- Cardiovascular Disease (1)
- Casein Matrix (1)
- CdSe (1)
- Cell transplantation (1)
- Climb (1)
- Cold-Water Immersion (1)
- Collateral Circuit (1)
- Collateral arteries (1)
- Collateral circulation (1)
- Computational Modeling (1)
- Conduction (1)
- Confocal Micrscopy (1)
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Articles 1 - 21 of 21
Full-Text Articles in Biomedical Engineering and Bioengineering
Using Noninvasive Calibrated Cuff Plethysmography To Observe The Effects Of Cold-Water Immersion On Arterial Compliance, Rita M. Grigorian
Using Noninvasive Calibrated Cuff Plethysmography To Observe The Effects Of Cold-Water Immersion On Arterial Compliance, Rita M. Grigorian
Master's Theses
As the prevalence of cardiovascular diseases continues to exponentially grow in populations across the globe, the necessity of determining underlying factors, effective methods of diagnoses, and universally available preventive measures also grows. Early detection of endothelial dysfunction, a proven precursor of cardiovascular diseases, can be extremely impactful in encouraging preventative measures and early intervention before medical conditions become chronic. In recent years, ice plunging, a form of cryotherapy involving full body immersion in cold water, has gained popularity within circles of fitness and health practitioners, gaining the interest of people of all backgrounds. Certain parallels observed between the human physiological …
Functionality Of The Gracilis Collateral Is Impaired Following Arteriogenesis And Cell Transplantation, Ada A. Tadeo
Functionality Of The Gracilis Collateral Is Impaired Following Arteriogenesis And Cell Transplantation, Ada A. Tadeo
Biomedical Engineering
Peripheral Arterial Disease involves narrowed arteries, reducing blood flow to limbs. Increasing blood flow to those extremities is possible by redirecting the blood to flow through natural bypasses (i.e. collateral arteries), which can enlarge via arteriogenesis to maintain blood supply once the prominent arteries have become occluded. This study aims to investigate how arteriogenesis affects collateral function, if myoblast transplantation can stimulate collateral growth, and how that in turn may affect collateral function. Femoral artery ligation was performed to mimic the blockage that occurs in patients with ischemic diseases on lean mice and mice with diet induced obesity (DIO). A …
Classifying Electrocardiogram With Machine Learning Techniques, Hillal Jarrar
Classifying Electrocardiogram With Machine Learning Techniques, Hillal Jarrar
Master's Theses
Classifying the electrocardiogram is of clinical importance because classification can be used to diagnose patients with cardiac arrhythmias. Many industries utilize machine learning techniques that consist of feature extraction methods followed by Naive- Bayesian classification in order to detect faults within machinery. Machine learning techniques that analyze vibrational machine data in a mechanical application may be used to analyze electrical data in a physiological application. Three of the most common feature extraction methods used to prepare machine vibration data for Naive-Bayesian classification are the Fourier transform, the Hilbert transform, and the Wavelet Packet transform. Each machine learning technique consists of …
Compositional Optimization Of Amyloid-Graphene Oxide Nanohybrids For Biomaterials, Claire L. Drewery
Compositional Optimization Of Amyloid-Graphene Oxide Nanohybrids For Biomaterials, Claire L. Drewery
Materials Engineering
Amyloid nanofibrils are natural materials capable of self-assembling into precise structures with tunable functionalities, while exhibiting excellent mechanical properties. In combination with highly conductive graphene oxide (GO), the 1-D amyloid nanofibrils and 2-D nanosheets of GO can produce a robust and bio-functional nanohybrid, hypothesized to exhibit multi-domain functional properties useful for enzyme sensing, water purification, drug delivery, and tissue scaffolding applications. Here, we examine the properties of an amyloid-graphene oxide nanohybrid film made with amyloids derived from hen egg white lysozymes in an attempt to explore the diverse toolbox of amyloid derivatives and establish ideal fabrication methods and formulations of …
Study Of Paper Microbial Fuel Cells For Use In On-Site Wastewater Testing, William A. Tolmasoff
Study Of Paper Microbial Fuel Cells For Use In On-Site Wastewater Testing, William A. Tolmasoff
Master's Theses
This study demonstrated a technique for fabricating simple, low-cost Paper Microbial fuel cells (PMFC’s) in the model of a previous study to, for the first time, produce voltage from wastewater effluent. The PMFC’s were created by stacking and gluing the main components of an MFC together: reservoir layer; anode; cation exchange membrane (CEM); air cathode. A wax printer was used to create the hydrophobic borders of the PMFC’s on filter paper, and graphite paint was applied to the paper to create the anode. The CEM’s considered were filter paper, wax, and Nafion, with Nafion being the most efficient. Finally, the …
Microfluidic Electrical Impedance Spectroscopy, John J. Foley
Microfluidic Electrical Impedance Spectroscopy, John J. Foley
Master's Theses
The goal of this study is to design and manufacture a microfluidic device capable of measuring changes in impedance valuesof microfluidic cell cultures. Tocharacterize this, an interdigitated array of electrodes was patterned over glass, where it was then bonded to a series of fluidic networks created in PDMS via soft lithography. The device measured ethanol impedance initially to show that values remain consistent over time. Impedance values of water and 1% wt. saltwater were compared to show that the device is able to detect changes in impedance, with up to a 60% reduction in electrical impedance in saltwater. Cells were …
Comparative Analysis Of Physiological Measurements And Environmental Metrics On Predicting Heat Stress Related Events, Mckenzie Lee Barlow
Comparative Analysis Of Physiological Measurements And Environmental Metrics On Predicting Heat Stress Related Events, Mckenzie Lee Barlow
Master's Theses
Exposure to high heat and humidity can lead to serious health risks, including heat exhaustion and heat stroke. Wet Bulb Globe Temperature (WBGT) and heat index have historically been used to predict heat stress events, but individualized factors are not included in the measurement. It has been shown that there is a relationship between cardiovascular measurements and heat stress, which could be used to measure heat stress risk on an individual level. Research has been done to find relationships between cardiovascular metrics in a workplace environment, however the study did not include the use of a controlled environment as a …
Prosthetic Socket Cooling System, Benjamin K. Kraw, Kathy Ha, Derek Piastrelli, Cuong Lai
Prosthetic Socket Cooling System, Benjamin K. Kraw, Kathy Ha, Derek Piastrelli, Cuong Lai
Mechanical Engineering
A team of four senior-level undergraduate students in the Interdisciplinary Senior Project Design course in the Engineering Department of California Polytechnic State University: San Luis Obispo have worked to develop a prosthetic socket cooling system, namely for veteran Taylor Morris and exclusively for a transfemoral prosthetic socket, under the Quality of Life+ Laboratory. This cooling system will utilize the thermoelectric effect known as the Peltier effect to transmit heat generated from the residual limb to the surrounding environment by means of forced convection over small aluminum heat sinks. Two measurement devices have also been developed to, one, measure the heat …
Developent Of A Phospholipid Encapsulation Process For Quantum Dots To Be Used In Biologic Applications, Logan Grimes
Developent Of A Phospholipid Encapsulation Process For Quantum Dots To Be Used In Biologic Applications, Logan Grimes
Master's Theses
The American Cancer Society predicts that 1,665,540 people will be diagnosed with cancer, and 585,720 people will die from cancer in 2014. One of the most common types of cancer in the United States is skin cancer. Melanoma alone is predicted to account for 10,000 of the cancer related deaths in 2014. As a highly mobile and aggressive form of cancer, melanoma is difficult to fight once it has metastasized through the body. Early detection in such varieties of cancer is critical in improving survival rates in afflicted patients. Present methods of detection rely on visual examination of suspicious regions …
Smooth Muscle Cell Organization In The Stem Region Of The Gracilis Collateral Circulation, Amanda Krall
Smooth Muscle Cell Organization In The Stem Region Of The Gracilis Collateral Circulation, Amanda Krall
Biomedical Engineering
Many patients who suffer from the ischemic Peripheral Arterial Occlusive Disease (PAOD) experience intermittent claudication, which can be attributed to impaired vasodilation. Collateral vessels are the primary site of resistance to blood flow downstream; therefore maximizing vasodilation in collaterals is crucial for efficient circulation. Collaterals function as natural bypasses around the occluded arteries and the increase in flow into these vessels causes them to outwardly remodel into conduit vessels. However, functional vasodilation in the stem region of collateral vessels is impaired at day 7 following femoral ligation, which can be attributed to smooth muscle cell malfunction. However, the increase in …
Life Support: Long Term Storage Of Solid Waste In An Enclosed Membrane System, Emmy O. Trieu, Michael Flynn, Rocco Mancinelli
Life Support: Long Term Storage Of Solid Waste In An Enclosed Membrane System, Emmy O. Trieu, Michael Flynn, Rocco Mancinelli
STAR Program Research Presentations
In deep space missions, maintaining life support is of the utmost priority. In such a closed system, human waste must be stored and treated. Simulated feces (ersatz) will be inoculated with microbes representing normal fecal flora, mixed with urine brine and shredded refuse of products typically used in space missions. Composting methods often use alternating layers of waste with scraps of carboniferous materials (finely shredded refuse). By preparing membrane bags with a homogenized ersatz and carboniferous refuse mixture and membrane bags with alternating layers of ersatz and carboniferous refuse, it may be possible to monitor anaerobic thermophillic digestion of the …
Design And Development Of A Stair Ascension Assistive Device For Transfemoral Amputees, Casey Michael Barbarino
Design And Development Of A Stair Ascension Assistive Device For Transfemoral Amputees, Casey Michael Barbarino
Master's Theses
Transfemoral amputees around the world experience increased difficulty in climbing stairs due to lack of muscle, balance, and other factors. The loss of a lower limb greatly diminishes the amount of natural force generation provided that is necessary to propel oneself up stairs. This study investigated possible solutions to the problem of stair ascension for transfemoral amputees by the means of designing and developing an externally attachable device to a prosthesis. The number of amputations from military service has greatly increased since 2008, which shows there is a clear need for assistive devices (Wenke, Krueger, & Ficke, 2012). With the …
Enhanced Hydrocarbon Biodegradation Using Bioaugmentation With Biowish™-Aqua Fog, Michael Robert Lehrer
Enhanced Hydrocarbon Biodegradation Using Bioaugmentation With Biowish™-Aqua Fog, Michael Robert Lehrer
Master's Theses
This study was done to determine the effectiveness of a commercially available bioaugmentation product, BiOWiSHTM-Aqua FOG, for remediating petroleum-contaminated sandy soil. Biodegradation enhancement by BiOWiSHTM-Aqua FOG was evaluated in laboratory microcosms by directly measuring total petroleum hydrocarbon (TPH) and indirectly using respirometry. Attempts were made to enrich hydrocarbon-degrading bacteria in BiOWiSHTM-Aqua FOG, and the resulting enrichment cultures were screened using respirometry as well. Potential hydrocarbon-degrading bacteria in BiOWiSHTM-Aqua FOG were isolated. Experiments were performed at bench-scale using microcosm bottles containing sand contaminated with either motor oil or No. 2 diesel fuel. The …
Development Of A Protocol To Measure Gene Expression In The Mouse Tibia, Daniel Hoover
Development Of A Protocol To Measure Gene Expression In The Mouse Tibia, Daniel Hoover
Biomedical Engineering
Numerous molecular factors active in bone tissue direct fracture repair and remodeling which can be altered by disease conditions such as Peripheral Arterial Disease (PAD) and Osteoporosis. Methods of molecular biology are commonly applied to investigate the expression and role of these molecular factors. This project presents a robust three-step protocol for examining gene expression in the mouse tibia. The protocol begins with isolating RNA from a flash frozen tibia sample. The isolated RNA is reverse transcribed into cDNA. Finally, PCR is performed to indentify expressed genes. Establishing this protocol will allow further research into the mechanisms of bone remodeling …
Impact Of Collateral Enlargement On Smooth Muscle Phenotype, Alexander Jerome Bynum
Impact Of Collateral Enlargement On Smooth Muscle Phenotype, Alexander Jerome Bynum
Master's Theses
Peripheral Artery Disease is a very serious disease characterized by an arterial occlusion due to atherosclerotic plaques. In response to an arterial occlusion, arteriogenesis occurs, causing smooth muscle cells to transition from a contractile to synthetic state. Also following an arterial occlusion, functional impairment was seen in the collateral circuit. An immunofluorescence protocol was developed in order to assess the impact of collateral enlargement (arteriogenesis) on smooth muscle phenotype at various time points. Smooth muscle α-actin was used to mark all smooth muscle cells, Ki-67 was used to label proliferating smooth muscle cells, and a fluorescent nuclear stain was used …
Impaired Resistance Artery Reactivity Following Arteriogenesis, Michael (Mike) Machado
Impaired Resistance Artery Reactivity Following Arteriogenesis, Michael (Mike) Machado
Biomedical Engineering
No abstract provided.
Forward Osmosis Bag: Ground Testing The Prospect Of Using Alternative Water Sources For Drinking Water, Michaela Marie Brant, Ariella Yendler, Mona Hammoudeh, Michael T. Flynn
Forward Osmosis Bag: Ground Testing The Prospect Of Using Alternative Water Sources For Drinking Water, Michaela Marie Brant, Ariella Yendler, Mona Hammoudeh, Michael T. Flynn
STAR Program Research Presentations
On the STS-135 Space Shuttle mission, to be launched July 8, 2011, a forward osmosis bag (FOB) study will be conducted. At NASA Ames this summer, the ground truth testing is being conducted for results comparisons. The FOB technology is derived from a commercial product, the X-Pack water filter. Forward osmosis operates by utilizing an established concentration gradient across a semi-permeable membrane to move water molecules from one side of the membrane to the other. This concept is exploited to harvest drinking water from grey water sources such as urine, sea water, or vehicle water. In this experiment, potassium chloride …
Determining A Method For Rendering Low Cost Cdse(Zns) Core(Shell) Quantum Dots Aqueous Soluble Via Amphiphilic Polymer Wrapping, Patrick Mcbride
Determining A Method For Rendering Low Cost Cdse(Zns) Core(Shell) Quantum Dots Aqueous Soluble Via Amphiphilic Polymer Wrapping, Patrick Mcbride
Materials Engineering
Herein is described the procedure of two amphiphilic polymer wrapping techniques that may be employed for obtaining aqueous soluble quantum dots (QDs) for use in biological fluorescent imaging applications. The advent of QDs has led to new nanoscale fluorescent materials that exhibit unparalleled quantum yields (QYs), high resistance to photobleaching, tunable emissions, and
absorption over a large optical range. However, the QD synthesis employed here at Cal Poly to obtain bright, photostable CdSe(ZnS) core(shell) QDs involves the use of organic solvents and surfactants, leading to hydrophobic QDs. Since all of biology relies on aqueous solubility, this hydrophobicity creates a major …
Studying Milk Coagulation Kinetics With Laser Scanning Confocal Microscopy, Image Processing, And Computational Modeling, Richard Joseph Hennessy
Studying Milk Coagulation Kinetics With Laser Scanning Confocal Microscopy, Image Processing, And Computational Modeling, Richard Joseph Hennessy
Master's Theses
The kinetics of milk coagulation are complex and still not well understood. A deeper understanding of coagulation and the impact of the relevant factors would aid in both cheese manufacturing and also in determining the nutritional benefits of dairy products. A method using confocal microscopy was developed to follow the movement of milk fat globules and the formation of a milk protein network during the enzyme-induced coagulation of milk. Image processing methods were then used to quantify the rate of coagulation. It was found that the texture of the protein network is an indicator of the current status of the …
Ischemia Impairs Vasodilation In Skeletal Muscle Resistance Artery, Kyle Remington Struthers
Ischemia Impairs Vasodilation In Skeletal Muscle Resistance Artery, Kyle Remington Struthers
Master's Theses
Functional vasodilation in arterioles is impaired with chronic ischemia. We sought to examine the impact of chronic ischemia and age on skeletal muscle resistance artery function. To examine the impact of chronic ischemia, the femoral artery was resected from young (2-3mo) and adult (6-7mo) mice and the profunda femoris artery diameter was measured at rest and following gracilis muscle contraction 14 days later using intravital microscopy. Functional vasodilation was significantly impaired in ischemic mice (14.4±4.6% vs. 137.8±14.3%, p<0.0001 n=8) and non-ischemic adult mice (103.0±9.4% vs. 137.8±14.3%, p=0.05 n=10). In order to analyze the cellular mechanisms of the impairment, a protocol was developed to apply pharmacological agents to the experimental preparation while maintaining tissue homeostasis. Endothelial and smooth muscle dependent vasodilation were impaired with ischemia, 39.6 ± 13.6% vs. 80.5 ± 11.4% and 43.0 ± 11.7% vs. 85.1 ± 10.5%, respectively. From this data, it can be supported that smooth muscle dysfunction is the reason for the observed impairment in arterial vasodilation.
A Novel Exercise Device For Users In Wheelchairs: A Study Of Abdominal Muscle Activation, Rebecca Jo Campbell
A Novel Exercise Device For Users In Wheelchairs: A Study Of Abdominal Muscle Activation, Rebecca Jo Campbell
Master's Theses
This study evaluates the use of a wheelchair balance board. The balance board was created as a sensory stimulation tool for users with various disabilities. It was originally designed to create vestibular stimulation for the person after they were loaded on. This study was used as a way to test if the balance board could be used for other things such as physical therapy and exercise. Ten able-bodied individuals were used to show the functionality of the device. They were asked to perform six different abdominal exercises while the muscle activity of their rectus abdominis and external obliques was measured …