Open Access. Powered by Scholars. Published by Universities.®
Biomedical Engineering and Bioengineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Ablation (1)
- Acetylcholine receptors (1)
- Airway smooth muscle (1)
- Calcium channels (1)
- Chemical elements (1)
-
- Contractions (1)
- Electrical properties and parameters (1)
- Electroporation (1)
- Electrotransfer (1)
- Emission spectroscopy (1)
- Gas liquid interfaces (1)
- Gene therapy (1)
- IL-12 (1)
- Immunotherapy (1)
- Irreversible electroporation (1)
- Melanoma (1)
- Microorganisms (1)
- Nanosecond pulse electric field (1)
- Nanosecond pulse stimulation (1)
- Penning ionization (1)
- Plasmas (1)
- Proteins (1)
- Voltage (1)
Articles 1 - 4 of 4
Full-Text Articles in Biomedical Engineering and Bioengineering
Il-12 Gene Electrotransfer Triggers A Change In Immune Response Within Mouse Tumors, Guilan Shi, Chelsea Edelblute, Sezgi Arpag, Cathryn Lundberg, Richard Heller
Il-12 Gene Electrotransfer Triggers A Change In Immune Response Within Mouse Tumors, Guilan Shi, Chelsea Edelblute, Sezgi Arpag, Cathryn Lundberg, Richard Heller
Bioelectrics Publications
Metastatic melanoma is an aggressive skin cancer with a relatively low survival rate. Immune-based therapies have shown promise in the treatment of melanoma, but overall complete response rates are still low. Previous studies have demonstrated the potential of plasmid IL-12 (pIL-12) delivered by gene electrotransfer (GET) to be an effective immunotherapy for melanoma. However, events occurring in the tumor microenvironment following delivery have not been delineated. Therefore, utilizing a B16F10 mouse melanoma model, we evaluated changes in the tumor microenvironment following delivery of pIL-12 using different GET parameters or injection of plasmid alone. The results revealed a unique immune cell …
Investigation On The Rons And Bactericidal Effects Induced By He + O2 Cold Plasma Jets: In Open Air And In An Airtight Chamber, Han Xu, Dingxin Liu, Weitao Wang, Zhijie Liu, Li Guo, Mingzhe Rong, Michael G. Kong
Investigation On The Rons And Bactericidal Effects Induced By He + O2 Cold Plasma Jets: In Open Air And In An Airtight Chamber, Han Xu, Dingxin Liu, Weitao Wang, Zhijie Liu, Li Guo, Mingzhe Rong, Michael G. Kong
Bioelectrics Publications
He + O2 plasma jets in open air and in an airtight chamber are comparatively studied, with respect to their production of gaseous/aqueous reactive species and their antibacterial effects. Under the same discharge power, the plasma jet in open air has higher densities of gaseous reactive species and a higher concentration of aqueous H2O2 but lower concentrations of aqueous OH and O2-. In addition, the increase in the O2 ratio in He in both plasma jets causes a linear decrease in the population of gaseous reactive species, except for O(3p5P) …
Voltage Effects On Muscarinic Acetylcholine Receptor-Mediated Contractions Of Airway Smooth Muscle, Iurii Semenov, Robert Brenner
Voltage Effects On Muscarinic Acetylcholine Receptor-Mediated Contractions Of Airway Smooth Muscle, Iurii Semenov, Robert Brenner
Bioelectrics Publications
Studies have shown that the activity of muscarinic receptors and their affinity to agonists are sensitive to membrane potential. It was reported that in airway smooth muscle (ASM) depolarization evoked by high K+ solution increases contractility through direct effects on M3 muscarinic receptors. In this study, we assessed the physiological relevance of voltage sensitivity of muscarinic receptors on ASM contractility. Our findings reveal that depolarization by high K+ solution induces contraction in intact mouse trachea predominantly through activation of acetylcholine release from embedded nerves, and to a lesser extent by direct effects on M3 receptors. We therefore devised …
Moderate Heat Application Enhances The Efficacy Of Nanosecond Pulse Stimulation For The Treatment Of Squamous Cell Carcinoma, Chelsea M. Edelblute, Sigi Guo, Embo Yang, Chunqi Jiang, Karl Schoenbach, Richard Heller
Moderate Heat Application Enhances The Efficacy Of Nanosecond Pulse Stimulation For The Treatment Of Squamous Cell Carcinoma, Chelsea M. Edelblute, Sigi Guo, Embo Yang, Chunqi Jiang, Karl Schoenbach, Richard Heller
Bioelectrics Publications
Nanosecond pulse stimulation as a tumor ablation therapy has been studied for the treatment of various carcinomas in animal models and has shown a significant survival benefit. In the current study, we found that moderate heating at 43°C for 2 minutes significantly enhanced in vitro nanosecond pulse stimulation-induced cell death of KLN205 murine squamous cell carcinoma cells by 2.43-fold at 600 V and by 2.32-fold at 900 V, as evidenced by propidium iodide uptake. Furthermore, the ablation zone in KLN205 cells placed in a 3-dimensional cell-culture model and pulsed at a voltage of 900 V at 43°C was 3 times …