Open Access. Powered by Scholars. Published by Universities.®
Biomedical Engineering and Bioengineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Adipic acid (1)
- Beta-ketoadipate pathway (1)
- Bio hydrogen beyond Thauer limit (1)
- Biofuel (1)
- Bioproduction (1)
-
- Colorimetric Assay (1)
- Continuous stirred tank reactor (1)
- Dark fermentation (1)
- Dynamic Light Scattering (1)
- ELISA (1)
- Factor XIII (1)
- Fibrinogen (1)
- Fibronectin (1)
- Hemostasis (1)
- Kinetic modeling (1)
- Lignin (1)
- Liquid Chromatography (1)
- Natural gas phase out (1)
- Pseudomonas kt2440 (1)
- Size Exclusion (1)
Articles 1 - 3 of 3
Full-Text Articles in Biomedical Engineering and Bioengineering
Bioproduction Of Adipic Acid Using Engineered Pseudomonas Putida Kt2440 From Lignin-Derived Aromatics, Howard Willett
Bioproduction Of Adipic Acid Using Engineered Pseudomonas Putida Kt2440 From Lignin-Derived Aromatics, Howard Willett
Department of Chemical and Biomolecular Engineering: Theses and Student Research
Current industrial synthesis of adipic acid is nonrenewable and depends on a carcinogenic starting material, benzene. Biocatalysis with an engineered microorganism could turn a renewable feedstock into a value-added chemical such as adipic acid. Here we engineered P. putida KT2440 to transform lignin-derived aromatics, coumarate and ferulate, into adipic acid. Lignin is a recalcitrant plant biopolymer burned for thermal energy. Conversion of lignin into a value-added chemical will improve the efficiency of lignocellulose processing plants. The best performing engineered KT2440 strain produces 2.52 mM adipate at a 9.5% (mole/mole) yield. This was achieved by the genetic insertion of non-natural biosynthetic …
Fibrinogen, Factor Xiii And Fibronectin: A Biophysical And Kinetic Characterization Of Their Interactions, Frank Fabian
Fibrinogen, Factor Xiii And Fibronectin: A Biophysical And Kinetic Characterization Of Their Interactions, Frank Fabian
Department of Chemical and Biomolecular Engineering: Theses and Student Research
The development of recombinant-based liquid fibrin tissue sealants having enhanced hemostatic and wound healing properties will involve understanding as yet not well characterized interactions between fibrinogen, fibrin (Fbn) factor XIII, thrombin and fibronectin. We study these phenomena in the context of comparing plasma derived fibrinogen to recombinant fibrinogen (rFI) produced in the milk of transgenic cows. An abundance of purified γγ and γγ’ FI subspecies enables detailed study of γγ or γγ’ biomonomer and their respective Fbn biopolymer formation as having different substrate behaviors of activated plasma derived factor XIII (pFXIIIa2b2). High pressure size exclusion (HPSEC) …
Comparative Kinetic Modeling Of Growth And Molecular Hydrogen Overproduction By Engineered Strains Of Thermotoga Maritima, Raghuveer Singh, Rahul Tevatia, Derrick White, Yaşar Demirel, Paul H. Blum
Comparative Kinetic Modeling Of Growth And Molecular Hydrogen Overproduction By Engineered Strains Of Thermotoga Maritima, Raghuveer Singh, Rahul Tevatia, Derrick White, Yaşar Demirel, Paul H. Blum
Department of Chemical and Biomolecular Engineering: Faculty Publications
Thermotoga maritima is an anaerobic hyperthermophilic bacterium known for its high amounts of hydrogen (H2) production. In the current study, the kinetic modeling was applied on the engineered strains of T. maritima that surpassed the natural H2 production limit. The study generated a kinetic model explaining H2 overproduction and predicted a continuous fermentation system. A Leudking-Piret equation-based model predicted that H2 production by Tma200 (0.217 mol-H2 g–1-biomass) and Tma100 (0.147 mol-H2 g–1-biomass) were higher than wild type (0.096 mol-H2 g–1 -biomass) with reduced rates of maltose utilization. …