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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Gene Delivery Through Cell Culture Substrate Adsorbed Dna Complexes, Zain Bengali, Angela K. Pannier, Tatiana Segura, Brian C. Anderson, Jae-Hyung Jang, Thomas D. Mustoe, Lonnie D Shea
Gene Delivery Through Cell Culture Substrate Adsorbed Dna Complexes, Zain Bengali, Angela K. Pannier, Tatiana Segura, Brian C. Anderson, Jae-Hyung Jang, Thomas D. Mustoe, Lonnie D Shea
Biological Systems Engineering: Papers and Publications
Efficient gene delivery is a fundamental goal of biotechnology and has numerous applications in both basic and applied science. Substrate-mediated delivery and reverse transfection enhance gene transfer by increasing the concentration of DNA in the cellular microenvironment through immobilizing a plasmid to a cell culture substrate prior to cell seeding. In this report, we examine gene delivery of plasmids that were complexed with cationic polymers (polyplexes) or lipids (lipoplexes) and subsequently immobilized to cell culture or biomaterial substrates by adsorption. Polyplexes and lipoplexes were adsorbed to either tissue culture polystyrene or serum-adsorbed tissue culture polystyrene. The quantity of DNA immobilized …
Interactions Of Bacteria And Amoebae With Ocular Biomaterials, Thomas John
Interactions Of Bacteria And Amoebae With Ocular Biomaterials, Thomas John
Cells and Materials
The use of biomaterials in periocular and intraocular sites has resulted in some ocular inflammations and infections which can result in vision-threatening ocular disease. This review addresses bacterial interactions with, and adherence to ocular biomaterials such as soft contact lenses, surgical suture materials, and intraocular lenses. In addition, adherence of Acanthamoeba to soft contact lenses is described, and the role of these lenses in the development of Acanthamoeba keratitis is discussed.