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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Comparative Assessment Of The Cytotoxicity Of Various Substrates In Organ Culture And Cell Culture: A Scanning Electron Microscopy Study, J. L. Duval, R. Warocquier-Clerout, M. F. Sigot-Luizard
Comparative Assessment Of The Cytotoxicity Of Various Substrates In Organ Culture And Cell Culture: A Scanning Electron Microscopy Study, J. L. Duval, R. Warocquier-Clerout, M. F. Sigot-Luizard
Cells and Materials
A comparative study of the behavior of chick embryo endothelial cells grown on various substrates was performed in order to establish the reliability and the limitation of both cell and organ culture methods. Following substrates were analyzed to compare these two different culture techniques: bovine serum albumin, pigskin gelatin and albumin + gelatin cross-linked by glutaraldehyde or carbodiimide, fibrin glue and negative control (Thermanox*). Parameters of cell growth and adhesion were calculated and compared with electron microscopic observations of cell morphology and of the extracellular matrix. Both culture methods provided complementary results and led to a similar classification of the …
Reactogenicity Of Biomaterials As Studied By Biochemical, Morphological And Ultrastructural Techniques, Leonid I. Slutskii, Natalya A. Sevastjanova, Ivetta L. Ozolanta, Irina V. Kuzmina, Laimdota E. Dombrovska
Reactogenicity Of Biomaterials As Studied By Biochemical, Morphological And Ultrastructural Techniques, Leonid I. Slutskii, Natalya A. Sevastjanova, Ivetta L. Ozolanta, Irina V. Kuzmina, Laimdota E. Dombrovska
Cells and Materials
Reactogenicity is a characteristic of biocompatible materials that provokes the reparative and proliferative reaction of connective tissues, a compulsory stage of which is inflammation. Thus, reactogenicity studies should include experiments in vivo. A quantitative assessment of reactogenicity can be obtained by subcutaneous implantation of standard olive-shaped specimens covered by the biomaterial under study followed by biochemical, histological and scanning electron microscopical studies of the capsule developing around the implant. Reactogenicity of surgical threads is evaluated by semiquantitative histological analysis of the wound healing process after suture application. Biomaterial reactogenicity can be modified by changing the structure of the surface and …