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Biomedical Engineering and Bioengineering Commons™
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- Biomaterials (3)
- Zebrafish (3)
- 3D printing (2)
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- Nanocellulose (2)
- Optics (2)
- 3D Printing (1)
- Academic Performance (1)
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- Animal models (1)
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Articles 1 - 21 of 21
Full-Text Articles in Biomedical Engineering and Bioengineering
Characterization Of Mammographic Dense Tissue Sub-Types Using Classification Algorithms On Metric Space Technique Output Functions, Madison Raza
Electronic Theses and Dissertations
In recent years breast cancer has become the leading cause of global cancer incidence. One of the most common forms of screening is through the use of digital x-ray screening mammography. Risk assessment models which help predict a patient’s risk of developing breast cancer rely mainly on patient history and qualitative breast density assessment from screening. The 2D wavelet transform maxima modulus (2D WTMM) method uses a sliding window approach to quantify the spatial organization of underlying mammographic tissue according to Hurst- exponent ranges (H) as fatty (H ≤ 0.45), healthy dense (H ≥ 0.55) and …
Development And Characterization Of Shear Thinning Cnf-Tempo Hydrogels For Use In Aquaculture Vaccines, Jacob A. Holbrook
Development And Characterization Of Shear Thinning Cnf-Tempo Hydrogels For Use In Aquaculture Vaccines, Jacob A. Holbrook
Electronic Theses and Dissertations
Aquaculture has shown significant growth within the past few decades and is expected to continue expanding rapidly as the global demand for fish consumption continues to increase. A significant loss within the aquaculture industry is due to pathogen transmission within the farm systems. These pathogens are mitigated through various methods that are not cost effective or have limited protection. The most common method of pathogen mitigation is through oil-based injection vaccines as they provide even protection across vaccinated fish and provides the longest protection compared to other vaccination methods. These oil-based injection vaccines are not without their flaws as they …
Designing And Modeling A Fail-Safe Mechanism To Be Used In Attachment Of A Transcutaneous Femoral Implant To A Prosthetic Device, Emma Sperry
Electronic Theses and Dissertations
Amputations are quite common and even modern prosthetic devices are plagued by problems. There are approximately 2 million people living with limb loss in the U.S. and on average 185,000 amputations occur yearly. Common attachment mechanisms for external prosthetic components to a residual limb, that is, sockets, pose numerous challenges. Issues include skin irritation, discomfort, socket fit issues, and immobility. Issues include skin irritation, discomfort, socket fit issues, and immobility. Transcutaneous implants have great potential as a connection method for external prosthetic components to a residual limb but because the implants are typically solid, they correlate to extremely high infection …
The Influence Of Processing And Additives On Cellulose Nanofiber Properties For Orthopedic Application, Mitchell P. Chesley
The Influence Of Processing And Additives On Cellulose Nanofiber Properties For Orthopedic Application, Mitchell P. Chesley
Electronic Theses and Dissertations
Current orthopedics are separated into three different classes of materials, metals, polymers, and ceramics. While these devices have had success throughout the years they are not without their faults. Metallic devices for example are usually extraordinarily stiff when compared with the surrounding bone. This difference in stiffness induces localized stress-shielding promoting cortical atrophy, which can lead to osteoporosis. Polymers while having the capacity of being biodegradable and bioabsorbable also have the potential to incite localized demineralization and weakness in surrounding bone. A result of breakdown byproducts not efficiently being evacuated from the area, which additionally acts as catalysts expediating the …
Nanocellulose Conduits For Enhanced Regeneration Of Peripheral Nerve Injuries, Nicklaus R. Carter
Nanocellulose Conduits For Enhanced Regeneration Of Peripheral Nerve Injuries, Nicklaus R. Carter
Electronic Theses and Dissertations
Peripheral neuropathy is estimated to afflict 20 million people in the United States. Most cases of neuropathy result from physical injuries and trauma arising from automobile accidents and war. Peripheral nerves have the intrinsic ability to regenerate over time, bridging the injury gap. However native regeneration is limited to a distance of only a few millimeters. Current methods utilized to assist in the regeneration of peripheral nerves over distances exceeding those amenable to native repair include nerve autografts and allografts, and implantation of conduits. Nerve autografts are regarded as the most effective method but require a second surgical site to …
Handling And Manipulation Of Water- And Air- Borne Biological Samples Using Liquid-Infused Surfaces, Daniel P. Regan
Handling And Manipulation Of Water- And Air- Borne Biological Samples Using Liquid-Infused Surfaces, Daniel P. Regan
Electronic Theses and Dissertations
Research on novel materials to handling water- and airborne samples for biological threats analysis is in great demand due to the COVID-19 pandemic. Work conducted on a new field of material science, called liquid-infused surfaces, demonstrate strong potential for the handling and manipulation of biological samples. As a result of the field’s infancy, only a limited number of studies have explored how liquid-infused surfaces can apply droplet manipulation strategies to address real-world problems. Presented in this dissertation are two platforms that leverage liquid-infused surfaces to address the challenges associated with handling water- and airborne biological samples. When dealing with waterborne …
Characterization Of Nano-Cellulose Based Composites For Biomedical Applications, Mitchell P. Chesley
Characterization Of Nano-Cellulose Based Composites For Biomedical Applications, Mitchell P. Chesley
Electronic Theses and Dissertations
The number of orthopedic surgeries performed globally has steadily increased over the past decade due to the standardization of procedures as well as technological advancements. During this time orthopedic devices have been composed predominantly of metals, such as Titanium, Vanadium, Molybdenum, and Stainless steel, as well as their alloys, due to the high strength and durability of these materials. However, metals may, in fact, be suboptimal for orthopedic devices. For example, metals exhibit Young’s modulus much greater than the surrounding bone, inducing localized stress-shielding promoting cortical atrophy, which can lead to osteoporosis. In recent years polymers have been successfully explored …
Design, Construction And Application Of A Home-Built, Two-Photon Microscope, William P. Breeding
Design, Construction And Application Of A Home-Built, Two-Photon Microscope, William P. Breeding
Electronic Theses and Dissertations
Two-photon microscopy (TPM) is a powerful, versatile imaging modality for the study of biological systems. This thesis overviews the relevant physics involved in TPM, design considerations and process of constructing a home-built, two-photon microscope, and provides a set of procedures to operate the system. Furthermore, this work explores several applications of TPM through the study of single-cell metabolism and imaging the cellular-material interface. Explored in particular depth was the imaging of cellulose nanofiber (CNF) materials, with the goal of understanding the three-dimensional nature of fibroblast cell growth when embedded within the materials. This work uncovered several optical properties of CNF, …
Implantation Of Nanocellulose In The Zebrafish Model, Hanna J. Anderson
Implantation Of Nanocellulose In The Zebrafish Model, Hanna J. Anderson
Honors College
The number of novel materials for use in biomedical implantation is expanding rapidly, increasing the success rates of implant procedures. Nanocellulose is being assessed as a sustainable and biocompatible material, offering an alternative to conventional polymer or metal designs with the appropriate structure for potential tissue integration. In this research, the capacity of cellulose nanofibers to serve as biomedical implants is assessed through examination of immune responses of transgenic zebrafish, utilizing bright field and confocal fluorescence microscopy. Methods for creating microincisions for the implantation of dense cellulose nanofiber shards in the zebrafish model were explored, and a surgical protocol was …
An Automated Device To Increase Screening Throughput Of Zebrafish Larvae, Fuoad Saliou-Sulley
An Automated Device To Increase Screening Throughput Of Zebrafish Larvae, Fuoad Saliou-Sulley
Electronic Theses and Dissertations
The use of the zebrafish as an animal model alternative to mammalian species has spawned research advancements in several medical fields. Since the zebrafish shares a high degree of sequence and functional homology with mammals, studies using this organism can provide in-depth insight into host response to disease and provide a platform for testing a range of treatment options. The optical transparency of zebrafish at early stages of development permits easy assessment of the effects of treatments, occurrence of tumors and other abnormal growth, disease progression, and immune response, to name only a few. These characteristics make it ideal for …
Stabilization Of Iron Oxide Nanoparticles For Homogenous Integration Into Cellulose Nano Fibrils, Banton H. Heithoff
Stabilization Of Iron Oxide Nanoparticles For Homogenous Integration Into Cellulose Nano Fibrils, Banton H. Heithoff
Honors College
Cellulose nanofibrils is one of the future potential giants in the medical implant industry. Its unique properties make it the ideal material for use in both permanent prosthetic devices and non- permanent implants such as screws and plates. To increase the usability of this material, the addition of super paramagnetic iron oxide nanoparticles is needed to gain MRI and X-Ray visibility. The methodology for how to homogeneously integrate these particles into the system using the addition of coating agents is explored. This research demonstrates that the addition of coating agents to the iron oxide nanoparticles can affect both the pH …
Host Range Of Mycobacteriophage And Exploring Lysogenic Relationships Between Mycobacteriophage And Their Hosts, Emily Illingworth
Host Range Of Mycobacteriophage And Exploring Lysogenic Relationships Between Mycobacteriophage And Their Hosts, Emily Illingworth
Honors College
Mycobacteriophage (phage) are abundant viruses that infect bacteria of the genus Mycobacterium. Pathogenic species of Mycobacterium carry prophage that are hypothesized to contribute to virulence. The term “prophage” describes a dormant phage during lysogeny, which occurs when a phage genome integrates itself into the host genome. Understanding the interactions between bacterial host and prophage may improve our ability to treat disease caused by bacteria. Phage are highly diverse and are sorted into clusters based on genome sequence similarity. To determine which phage infect and form lysogens in mycobacterial fish pathogens, we applied viral dosages of phage representative of clusters A–X …
Development Of Spectroscopic Methods For Dynamic Cellular Level Study Of Biochemical Kinetics And Disease Progression, Anna M. Sitarski
Development Of Spectroscopic Methods For Dynamic Cellular Level Study Of Biochemical Kinetics And Disease Progression, Anna M. Sitarski
Electronic Theses and Dissertations
One of the current fundamental objectives in biomedical research is understanding molecular and cellular mechanisms of disease progression. Recent work in genetics support the stochastic nature of disease progression on the single cell level. For example, recent work has demonstrated that cancer as a disease state is reached after the accumulation of damages that result in genetic errors. Other diseases like Huntingtons, Parkinsons, Alzheimers, cardiovascular disease are developed over time and their cellular mechanisms of disease transition are largely unknown. Modern techniques of disease characterization are perturbative, invasive and fully destructive to biological samples. Many methods need a probe or …
A Multi-Channel 3d-Printed Bioreactor For Evaluation Of Growth And Production In The Microalga Dunaliella Sp, Cristian A. Cox
A Multi-Channel 3d-Printed Bioreactor For Evaluation Of Growth And Production In The Microalga Dunaliella Sp, Cristian A. Cox
Electronic Theses and Dissertations
We explored the capabilities of additive manufacturing using a photo-cured jetted material 3D printer to manufacture a milli-microfluidic device with direct application in microalgae Dunaliella sp growth and intracellular compounds biosynthesis tests. A continuous microbioreactor for microalgae culture was CAD designed and successfully built in 1 hour and 49 minutes using black photopolymer cured by UV and a support material. The microreactor was made up of 2 parts including the bioreactor itself and a microchannel network for culture media fluids and microalgae. Both parts were assembled to form a single unit. Additional optical and auxiliar components were added. An external …
Citrate’S Counter Ions And Ph Effect On Gold Nanoparticle Growth Kinetics, Andrew B. Closson
Citrate’S Counter Ions And Ph Effect On Gold Nanoparticle Growth Kinetics, Andrew B. Closson
Honors College
The effects of citrate’s counterions and pH are examined in gold nanoparticle growth kinetics. The growth of gold nanoparticles from citrate reduction is a common method used for creating particles from 10-120 nm with a well-defined size distribution. TEM observation of the seed-mediated growth shows an intermediate aggregation phase that has been shown by researchers to exist in other solution-phase nanocrystal growth. We have shown that this intermediate agglomeration phase within particle growth may be avoided with certain pairings of counterion and pH. This aggregation phase has no noticeable effects on the formation of the particles at a smaller scale …
The Role Of Ship1 In The Innate Immune System During An Influenza A Viral Infection, Spencer Earle Traxler
The Role Of Ship1 In The Innate Immune System During An Influenza A Viral Infection, Spencer Earle Traxler
Honors College
The SHIP1 gene is a member of the inositol polyphosphate-5-phosphate (INPP5) and its expressed protein functions as a negative regulator of myeloid cell proliferation, survival, and migration. Mutations in this gene are associated with various defects and cancers of the immune system. Previous studies have shown that in response to wound formation, ship1-deficient zebrafish have increased neutrophil motility while overexpression of ship1 resulted in decreased neutrophil migration (Lam et al. 2012). From this research, it is suggested that SHIP1 is a key brake that limits neutrophil motility through a PI3K signaling-dependent pathway. While the role of SHIP1 during a wound …
Optimization And Synthesis Of Silver Nanoparticles Embedded Within A Porous Substrate For Raman Spectroscopy, Matthew W. Talbot
Optimization And Synthesis Of Silver Nanoparticles Embedded Within A Porous Substrate For Raman Spectroscopy, Matthew W. Talbot
Honors College
Raman spectroscopy is a promising method for detection of a wide range of water contaminants. Raman spectroscopy’s growing list of applications relies upon signal enhancement achieved in recent years. A test strip or substrate designed to optimize Raman spectra, capable of withholding water and enhancing signal, would be a useful tool for applications including water quality tests. Signal enhancement may be achieved by the addition of silver nanoparticles (NPs) into a three-dimensional structure of cellulose nanofibers (CNF). The magnitude of signal enhancement may be related to nanoparticle size and morphology, and so control over the synthesis of silver nanoparticles could …
Investigating The Optimization Of Zebrafish (Danio Rerio) Dechorionation For High-Throughput Applications In Influenza Research, Thomas Hoffmann
Investigating The Optimization Of Zebrafish (Danio Rerio) Dechorionation For High-Throughput Applications In Influenza Research, Thomas Hoffmann
Honors College
No abstract provided.
Substrate Stiffness And Adhesivity Influence Neuron Axonal Growth, Eben Grant Estell
Substrate Stiffness And Adhesivity Influence Neuron Axonal Growth, Eben Grant Estell
Honors College
The nervous system is a complex organ system that coordinates all of the actions of an animal. The transmission of signals between different body parts by this organ system allows the body to function as a whole and interact with the external environment. It is clear then that the nervous system is integral to the function and survival of those organisms in which it has developed; and yet much is lacking in our understanding of the system’s key constituents: neuronal cells or, neurons. In satisfaction of the Honors Thesis, this research investigates how certain conditions of the extracellular environment …
Development Of Porous Polymeric Implants For Use In Orthopedic Research And Development Applications, Alexander Caddell
Development Of Porous Polymeric Implants For Use In Orthopedic Research And Development Applications, Alexander Caddell
Honors College
Current orthopedic implants comprised of plastic, ceramic, or metal alloys are susceptible to surface degradation at the implant-implant interface. The resulting microscopic fragments cause tissue irritation that can lead to osteolysis. In addition, existing percutaneous implants, such as pins used to stabilize fractures, are prone to bacterial infections due to the inability of the surrounding soft tissue to adhere to the implant and form a biologic seal. The goal of this Honors Thesis was to develop porous polymeric implants for orthopedic research and development applications that improve upon current designs in an attempt to remedy the issues detailed above. A …
Anaerobic Fermentation Of Hemicellulose Present In Green Liquor And Hot Water Extracts To Carboxylic Acids, Rakhi Reddy Baddam
Anaerobic Fermentation Of Hemicellulose Present In Green Liquor And Hot Water Extracts To Carboxylic Acids, Rakhi Reddy Baddam
Electronic Theses and Dissertations
Wood is composed of cellulose, hemicellulose and lignin. In the paper industry the cellulose fraction is the major resource used in paper production, whereas the hemicellulose and the lignin are usually burned for heat recovery. Recently, wood-derived hemicellulose extracts have received much attention for the production of valuable bioproduct fuels and chemicals. Mixed-culture microbial ecosystems are capable of converting biomass materials, such as wood extracts, into mixtures of carboxylic acids (C1-C7), which can in turn be purified and sold as products, or converted into other organic chemicals through chemical means. The relative concentrations of the acids produced in the fermentations …