Biomedical Engineering and Bioengineering Commons^™

Monoclonal Antibody Assay And Kit For Detecting Metal Cations In Body Fluids (Continuation), Randall R. Carlson, Jay S. Stout, Dwane E. Wylie, Fred W. Wagner, Malcolm Riddell

Biological Process Development Facility: Staff Publications

The invention provides method and kits for detecting a metallic cation in a sample of a body fluid. The preferred method and kits include the use of at least two different types of antibodies having different specificities. In the preferred method, the sample of body fluid can be contacted with an effective amount of a capture antibody specific for a naturally occurring polypeptide that can bind the metallic cation to form a first antigen-antibody complex. An effective amount of an antibody specific for an epitope on a metallic cation-naturally occurring polypeptide complex or an antibody specific for a metallic cation …

Monoclonal Antibody Assay And Kit For Detecting Metal Cations In Body Fluids, Randall R. Carlson, Jay S. Stout, Dwane E. Wylie, Fred W. Wagner, Malcolm Riddell

Biological Process Development Facility: Staff Publications

The invention provides method and kits for detecting a metallic cation in a sample of a body fluid. The preferred method and kits include the use of at least two different types of antibodies having different specificities. In the preferred method, the sample of body fluid can be contacted with an effective amount of a capture antibody specific for a naturally occurring polypeptide that can bind the metallic cation to form a first antigen-antibody complex. An effective amount of an antibody specific for an epitope on a metallic cation-naturally occurring polypeptide complex or an antibody specific for a metallic cation …

A Timesaving Method For Labeling Figures, Tables And References, Randy Carlson

Biological Process Development Facility: Staff Publications

No matter how carefully an author outlines a manuscript, the figures, tables, and bibliographic citations are inevitably renumbered one or more times before the final draft. For example, the addition of a new reference to a late draft will sometimes entail the renumbering of all other references throughout the manuscript. A partial solution to this common technical problem in scientific writing is offered here. It is a refinement of an approach known to many scientists.

Taxonomy Of Corynebacterium Plant Pathogens, Including A New Pathogen Of Wheat, Based On Polyacrylamide Gel Electrophoresis Of Cellular Proteins, Randall R. Carlson, Anne K. Vidaver

Biological Process Development Facility: Staff Publications

The known extant plant pathogenic Corynebacterium species were analyzed by polyacrylamide gel electrophoresis of their cellular proteins. The patterns of the protein bands of 13 species and a new corynebacterial wheat pathogen showed seven distinct groups. Five of these groups consisted of only one species each, one group contained four species, and the last group contained the new wheat pathogen and the remaining four species. The pathogens that could not be distinguished by the polyacrylamide gel analysis differed in phenotypic characteristics, including pathogenic specificity. Thus, for these bacteria we propose recognition of the following taxa: Corynebacterium fascians (Tilford) Dowson, Corynebacterium …

Bacterial Mosaic, A New Corynebacterial Disease Of Wheat, Randall R. Carlson, Anne K. Vidaver

Biological Process Development Facility: Staff Publications

Bacterial mosaic is a foliar disease of wheat; characteristic symptoms are small yellow lesions, more or less uniformly distributed over the leaf. It was discovered in Nebraska in the spring of 1976. By 1979, the pathogen was distributed over an 800-km range and had been isolated from 16 cultivars of winter wheat. The pathogen is Corynebacterium michiganense subsp. tessellarius, a Gram-positive coryneform. Wheat was the only host that showed symptoms after inoculation. The pathogen reached high population levels (> 10' colony-forming units per gram of fresh weight) without apparent symptoms in tomato and in six of nine gramineous plants …

A Pressure Injection Device For Inoculation Of Maize With Bacterial Phytopathogens, Randall R. Carlson, A. K. Vidaver, D. S. Wysong, J. H. Riesselman

Biological Process Development Facility: Staff Publications

A pressure injection device (PID) was used to inoculate maize (Zea mays) with bacterial phytopathogens. The PID was evaluated for its speed, effectiveness, and precision relative to the needle-eye method of inoculation. Corynebacterium nebraskense, Erwinia stewartii, and Pseudomonas andropogonis were each used successfully as inocula to infect maize and produce symptoms typical of Goss's wilt, Stewart's wilt and bacterial stripe, respectively. Quantitative inoculation with the PID was faster and as statistically precise as using a calibrated needle-eye.

Leaf Spot Of Field Corn Caused By Pseudomonas Andropogonis, Anne K. Vidaver, Randall R. Carlson

Biological Process Development Facility: Staff Publications

A leaf spot of field corn was shown to be caused by the bacterium Pseudomonas andropogonis. Leaf spot symptoms were observed over 3 years in several States. Inoculation of corn by vacuum infiltration of the bacteria was necessary to reproduce field symptoms.

A bacterial leaf spot disease of different cultivars of field corn was observed in early summer of 1973, 1974, and 1975. Leaves with essentially the same symptoms were obtained from South Dakota, Iowa, Kansas, Nebraska, and Michigan. The same symptoms were also seen in Wisconsin. Symptoms consisted of circular to ellipsoidal, tan to brown spots, with irregular …