Engineering Commons^™

Magnetic Nanoparticles Enhanced Surface Plasmon Resonance Biosensor For Rapid Detection Of Salmonella Typhimurium In Romaine Lettuce, Devendra Bhandari, Fur-Chi Chen, Roger C. Bridgman

Human Sciences Faculty Research

Salmonella is one of the major foodborne pathogens responsible for many cases of illnesses, hospitalizations and deaths worldwide. Although different methods are available to timely detect Salmonella in foods, surface plasmon resonance (SPR) has the benefit of real-time detection with a high sensitivity and specificity. The purpose of this study was to develop an SPR method in conjunction with magnetic nanoparticles (MNPs) for the rapid detection of Salmonella Typhimurium. The assay utilizes a pair of well-characterized, flagellin-specific monoclonal antibodies; one is immobilized on the sensor surface and the other is coupled to the MNPs. Samples of romaine lettuce contaminated with …

Extended Lamer Synthesis Of Cobalt Doped Ferrite, Benjamin D. Fellows, Sarah Sandler, Jacob Livingston, Kristin Fuller, Lotanna Nwandu, Sarah Timmis, Kayla A. Lantz, Morgan Stefik, O. Thompson Mefford

Publications

Nonstoichiometric cobalt ferrite nanoparticles have drawn interest in magnetically mediated energy delivery due to their high magnetocrystalline anisotropy and their high peak loss frequency. The use of an extended LaMer synthesis allows for size control of the doped particles up to a threshold core diameter of 18 nm. Above this diameter, particles become unstable and drop out of suspension, allowing for further nucleation events to occur. This leads to a cyclic nucleation, growth, and destabilization regime seen during the course of the reaction. Using the size control of the extended LaMer synthesis technique, we examine the specific absorption rate (SAR) …

Photopolymerization Synthesis Of Magnetic Nanoparticle Embedded Nanogels For Targeted Biotherapeutic Delivery, Daniel Jonwal Denmark

USF Tampa Graduate Theses and Dissertations

Conventional therapeutic techniques treat the patient by delivering a biotherapeutic to the entire body rather than the target tissue. In the case of chemotherapy, the biotherapeutic is a drug that kills healthy and diseased cells indiscriminately which can lead to undesirable side effects. With targeted delivery, biotherapeutics can be delivered directly to the diseased tissue significantly reducing exposure to otherwise healthy tissue. Typical composite delivery devices are minimally composed of a stimuli responsive polymer, such as poly(N-isopropylacrylamide), allowing for triggered release when heated beyond approximately 32 °C, and magnetic nanoparticles which enable targeting as well as provide a mechanism for …

Design And Synthesis Of Polymer - Magnetic Nanoparticle Composites For Use In Biomedical Applications, Roland Stone

All Dissertations

The future of diagnostics and therapeutic drugs in biomedicine is nanoparticles. These nanoparticles come in many different shapes, sizes, and combination of materials. Magnetic nanoparticles have been studied for many years for use in biomedicine, not only for their high surface area, but also because of its unique magnetic properties. They can magnetically interact with their environment, be guided to a specific location, and manipulated to release energy in the form of heat. To ensure that these magnetic nanoparticles survive in the circulatory system, they must be modified with materials to make them colloidally stable in water and shield them …

Spatial Control Of Magnetic Nanoparticles Integrated With Cellular Spheroids As Tissue Engineered Building Blocks, Brandon Mattix

All Dissertations

Magnetic nanoparticles (MNPs) have been investigated in tissue engineering applications to provide in situ imaging, drug delivery, and tissue patterning, but direct and prolonged interaction between cells and MNPs can have adverse effects on cell function. Therefore, methods which reduce or limit the interaction of MNPs with cells, or utilize more biocompatible MNP-based strategies will improve upon the commonly used iron oxide MNPs. We investigated a variety of methods to improve upon the use of MNPS in tissue engineering.
Cell aggregates, or spheroids, have been used as tissue engineered building blocks that can closely mimic the native three-dimensional in vivo …

Nanomaterials For Biological Applications: Drug Delivery And Bio-Sensing, Hui Ma

University of New Orleans Theses and Dissertations

The idea of utilizing nanomaterials in bio-related applications has been extensively practiced during the recent decades. Magnetic nanoparticles (MPs), especially superparamagnetic iron oxide nanoparticles have been demonstrated as promising candidates for biomedicine. A protective coating process with biocompatible materials is commonly performed on MPs to further enhance their colloidal and chemical stability in the physiological environment. Mesoporous hollow silica is another class of important nanomaterials that are extensively studied in drug delivery area for their ability to carry significant amount of guest molecules and release in a controlled manner.

In this study, different synthetic approaches that are able to produce …

Temperature-Induced Phenomena In Systems Of Magnetic Nanoparticles, Abdul Wazed Bhuiya

Open Access Theses & Dissertations

Magnetic nanoparticle ensembles have received a lot of attention, stemming in part from their current and potential applications in biomedicine and in the development of high-density magnetic storage media. Key to the functionality of these systems are microscopic structures and mechanisms that make them exhibit unique properties and behave differently from their bulk counterparts.

We studied microscopic structures and processes that dictate macroscopic properties, behavior and functionality of magnetic nanoparticle ensembles. As the temperature T strongly influences the magnetic behavior of these systems, we studied temperature dependent magnetic properties using AC-susceptibility and DC-magnetization measurements carried out over a broad range …