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Full-Text Articles in Engineering
A Quantitative, Technology Independent, Fidelity Metric For Evaluating Bioprinted Patterned Co-Cultures, Matthew Pepper
A Quantitative, Technology Independent, Fidelity Metric For Evaluating Bioprinted Patterned Co-Cultures, Matthew Pepper
All Theses
The study of cell-cell interactions is crucial in the understanding of cell behaviors such as tumor genesis, proliferation, migration, metastasis, and apoptosis. To break down the complex web of signals in vivo, researchers must replicate some parts of this environment with in vitro tissue test systems, composed of multiple cell types arranged close enough to communicate with their neighbors, i.e. high-resolution co-culture patterns. The field of bioprinting is specifically focused on creating co-culture patterns for the purposes of cell studies, but the sample resolutions of most bioprinting systems are still too coarse to permit cell communication. No way currently exists …
Manipulating Cellular Growth Responses To Patterning, Apoptotic, And Environmental Cues, James N. Mcnamara
Manipulating Cellular Growth Responses To Patterning, Apoptotic, And Environmental Cues, James N. Mcnamara
Doctoral Dissertations
In this work, various methods of controlling cell growth are examined. Cell-cell interaction, apoptotic cues, three dimensional scaffolds, and non-adherent environments are evaluated for their ability to affect the differentiation, morphology, and growth rate of different cell types. Previous work has shown that cell growth and cell morphology can be influenced by patterns of polymers coated on surfaces in two dimensions, designated here as the x and y dimensions of a standard Cartesian coordinate system. Tissue engineering and regenerative medicine studies have shown limited success in modifying growth in the third, z dimension. This work considers not only the x, …
Creation Of Defined Single Cell Resolution Neuronal Circuits On Microelectrode Arrays, Russell Pirlo
Creation Of Defined Single Cell Resolution Neuronal Circuits On Microelectrode Arrays, Russell Pirlo
All Dissertations
The way cell-cell organization of neuronal networks influences activity and facilitates function is not well understood. Microelectrode arrays (MEAs) and advancing cell patterning technologies have enabled access to and control of in vitro neuronal networks spawning much new research in neuroscience and neuroengineering. We propose that small, simple networks of neurons with defined circuitry may serve as valuable research models where every connection can be analyzed, controlled and manipulated.
Towards the goal of creating such neuronal networks we have applied microfabricated elastomeric membranes, surface modification and our unique laser cell patterning system to create defined neuronal circuits with single-cell precision …
Application Of Polyelectrolyte Multilayers For Photolithographic Patterning Of Diverse Mammalian Cell Types In Serum Free Medium, Vipra Dhir
Electronic Theses and Dissertations
Integration of living cells with novel microdevices requires the development of innovative technologies for manipulating cells. Chemical surface patterning has been proven as an effective method to control the attachment and growth of diverse cell populations. Patterning polyelectrolyte multilayers through the combination of layer-by-layer self-assembly technique and photolithography offers a simple, versatile and silicon compatible approach that overcomes chemical surface patterning limitations, such as short-term stability and low protein adsorption resistance. In this study, direct photolithographic patterning of PAA/PAAm and PAA/PAH polyelectrolyte multilayers was developed to pattern mammalian neuronal, skeletal and cardiac muscle cells. For all studied cell types, PAA/PAAm …