Engineering Commons^™

Method Development For The Extraction And Analysis Of Per- And Polyfluoroalkyl Substances (Pfas) In Wastewater And Biosolids, Victoria Krull

All Graduate Theses and Dissertations, Fall 2023 to Present

Per and poly-fluorinated alkyl substances (PFAS) are manufactured chemicals that have water and grease repellant properties and do not readily biodegrade. PFAS are present in many consumer products and end up in waste systems and the environment. Studies have shown that PFAS can have adverse health effects on humans and animals.

There are many challenges to processing and analyzing samples containing PFAS including: adsorption of PFAS to equipment, contamination from equipment containing PFAS, and the high sensitivity needed to analyze for PFAS in the parts per trillion detection range. The United States Environmental Protection Agency (EPA) has been developing methods …

Tracking Perfluoroalkyl Substances From Wastewater Influent To Its Accumulation In Vegetables And Forage Grass, Simon Kozik

All Graduate Theses and Dissertations, Fall 2023 to Present

Per and poly-fluorinated alkyl substances (PFAS) are a class of chemicals that are persistent in the environment. PFAS was found in wastewater, which can be used after wastewater treatment for irrigation water, and in the biosolids that can be mixed with soils to provide nutrients and generally improve soil quality. This study found when wastewater was used it increases the level of PFAS in vegetables and forage grass. PFAS concentrations were tracked from the influent of the wastewater treatment plant to the effluent, to the irrigation water, to the soil, and finally into the vegetable and forage grass grown on …

Pyrolysis—A Tool In The Wastewater Solids Handling Portfolio, Not A Silver Bullet: Benefits, Drawbacks, And Future Directions, Patrick J. Mcnamara, Zhongzhe Liu, Yiran Tong, Hari Santha, Lynne Moss, Daniel Zitomer

Civil and Environmental Engineering Faculty Research and Publications

Pyrolysis is the process whereby carbonaceous materials, such as biosolids, are heated between 400°C and 900°C in the absence of oxygen. Three main products are generated: a solid product called biochar, a py-liquid that consists of aqueous phase and non-aqueous phase liquid, and py-gas. The biochar holds value as a beneficial soil amendment and sequesters carbon. The py-liquid is potentially hazardous and needs to be dealt with (including potentially reducing it on-site via catalysis or thermal oxidation). Py-gas can be used on-site for energy recovery. Pyrolysis has gained recent interest due to concern over per- and polyfluoroalkyl substances (PFAS) in …

Occurrence Of Per- And Polyfluoroalkyl Substances (Pfas) In New Hampshire Biosolids, Katherine A. Wieck

Honors Theses and Capstones

Per- and polyfluoroalkyl substances (PFAS) are a group of over 4,000 compounds used in the manufacturing of products including aqueous film forming foams for firefighting, stain repellents, waterproofing agents, and nonstick cookware since their initial development in the 1940s. The long fluorinated carbon chain structure of PFAS causes chemical and thermal stability, and thus resistance to biodegradation. Biosolids produced at wastewater facilities for uses such as agricultural land-applied compost and fertilizer for lawns and athletic fields, as well as sludge disposed in landfills can cause contamination of groundwater and surface water. This poses a significant threat to human and environmental …

Mechanisms Of Pathogen Inactivation In Wastewater And Pharmaceutical Applications, Christa L. Meingast

Dissertations, Master's Theses and Master's Reports

Infectious diseases are a significant threat to public health. Though society enacts practices to prevent the spread of these dangerous diseases, challenges remain. Therefore, continual advancements in treatment and prevention are required. Wastewater treatment and viral clearance in pharmaceutical applications are two key health measures that prevent the spread of infections.

A low-cost, low-technology biosolids treatment process was developed to improve wastewater treatment by collecting key information on storage temperature, ammonia, volatile solids, moisture content, pH, and pathogen inactivation in biosolids over long-term storage at two wastewater treatment plants located in northern climates of the United States. Inactivation of pathogens …

Kinetic Analysis Of Biosolid Pyrolysis, William Regan Kreutter

Master's Theses (2009 -)

Waste reduction and energy recovery have been an environmental focus. Many of these solutions involve the thermal degradation of waste, such as household garbage or organic waste. To help reduce the negative environmental impact associated with processes like incineration, methods have been developed to utilize the carbonaceous material and energy contained in waste. Wastewater treatment plants are responsible for collecting and cleaning billions of gallons of sewage and stormwater each year. The water collected goes through multiple cleaning stages before being discharged into surface water. Sewage sludge, commonly referred to as biosolids, are produced during the process. Biosolids are carbon …

Modification Of Gleams For Modeling Movement Of Organic Contaminants From Land-Applied Biosolids, Ben D. Giudice, Thomas M. Young, Jacob P. Bibb

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

Municipal biosolids are commonly applied to agricultural lands as fertilizer, but this also poses potential risks to groundwater and surface water quality from constituents that may be mobilized during storm events. In the present study, an existing model, Groundwater Loading Effects of Agricultural Management Systems (GLEAMS), is modified to predict the fate and transport of organic contaminants from land-applied biosolids, primarily via addition of a labile biosolids organic carbon phase distinct from soil organic carbon. While capable of simulating contaminant transport in runoff and via percolation, only the runoff portion of the model was able to be calibrated using existing …

Improved Methods For The Quantification Of Viable Ascaris Suum And Application To Biosolids, Tanner Keyzers

Dissertations, Master's Theses and Master's Reports

The current method for detection of Ascaris ova in biosolids and wastewater has several drawbacks including being labor and time intensive as well as being difficult to quantify viable ova that have larvated during treatment. The goal of this thesis was to improve several methods for the detection and quantification of Ascaris ova during long term-storage of biosolids including improvement of quantifying viable ova using a heat and bleach treatment to promote movement of larvae inside of ova, and comparison of the traditional microscopic method with qPCR and PMA-qPCR to prevent the false-positive results. Improved methods to promote movement of …

Pathogen And Indicator Organism Inactivation And Quantification For Low-Cost Low-Tech Biosolids Treatment, Karina Eyre

Dissertations, Master's Theses and Master's Reports

Biosolids are a by-product of municipal wastewater treatment which, when treated to reduce pathogens, can be land applied as a fertilizer. Class A biosolids must contain low pathogen levels and are unrestricted in their use. This thesis describes methods to identify pathogen and indicator organism (PIO) inactivation mechanisms and PIO inactivation rates for use in low-cost low-tech Class A processes. Procedures to control the levels of spiked bacteria and viruses, total solids, volatile fatty acids, pH, and temperature in biosolids are described for their use in laboratory experiments. Methods for coliphage in biosolids as a surrogate for total enteric viruses …

A Preliminary Sustainability Analysis Of Producing Class A Biosolids With Conventional And Low-Cost, Low-Tech Processes, Janna Brown

Dissertations, Master's Theses and Master's Reports

Producing Class A biosolids is a beneficial way to reuse wastewater sludge. Low-cost, low-tech (LCLT) processes are attractive to small water resource recovery facilities (WRRF) because of their low capital costs and simple designs. This study examined the sustainability of conventional and LCLT processes at small WRRFs. The environmental impacts were determined by conducting life cycle assessments. Cost analyses determined the economic impacts. The social impacts were analyzed by investigating case studies and surveys of social response to biosolids. The environmental, economic, and social impacts were normalized, and each technology received an overall score. The technologies studied were direct heat …

A Holistic Process Model For Evaluating Alternative Biosolids Technologies And Operational Methodologies To Improve Performance And Sustainable Decision-Making At High Purity Oxygen Wastewater Treatment Plants, Manuel E. Moncholi

FIU Electronic Theses and Dissertations

In the span of the last decade the regulatory, public and commercial sentiments in Florida towards wastewater biological sludges, termed biosolids, have resulted in more restrictive land application rules, public cynicism about quality and agricultural benefits, and defensive posturing and marketing to appease public opinion. To determine and recommend the most cost effective and sustainable combination of biosolids treatment technologies to achieve Class B biosolids at the MDWASD facilities, the author created a holistic process model that is novel in the realm of wastewater treatment.

The research methodology focused on the analyses of historical data, analyses of pilot-testing data, development …

Metagenomics Reveal Triclosan-Induced Changes In The Antibiotic Resistome Of Anaerobic Digesters, M. Fujimoto, Daniel Elliott Carey, Patrick J. Mcnamara

Civil and Environmental Engineering Faculty Research and Publications

Triclosan (TCS) is a broad-spectrum antimicrobial used in a variety of consumer products. While it was recently banned from hand soaps in the US, it is still a key ingredient in a top-selling toothpaste. TCS is a hydrophobic micropollutant that is recalcitrant under anaerobic digestion thereby resulting in high TCS concentrations in biosolids. The objective of this study was to determine the impact of TCS on the antibiotic resistome and potential cross-protection in lab-scale anaerobic digesters using shotgun metagenomics. It was hypothesized that metagenomics would reveal selection for antibiotic resistance genes (ARGs) not previously found in pure culture studies or …

Characteristics And Applications Of Biochars Derived From Wastewater Solids, Zhongzhe Liu, Simcha L. Singer, Yiran Tong, Lee Kimball, Erik Anderson, Matthew Hughes, Daniel Zitomer, Patrick J. Mcnamara

Civil and Environmental Engineering Faculty Research and Publications

Pyrolysis is a thermochemical decomposition process that can be used to generate pyrolysis gas (py-gas), bio-oil, and biochar as well as energy from biomass. Biomass from agricultural waste and other plant-based materials has been the predominant pyrolysis research focus. Water resource recovery facilities also produce biomass, referred to as wastewater solids, that could be a viable pyrolysis feedstock. Water resource recovery facilities are central collection and production sites for wastewater solids. While the utilization of biochar from a variety of biomass types has been extensively studied, the utilization of wastewater biochars has not been reviewed in detail. This review compares …

The Impact Of Phosphorous Species On Dewaterability Of Wastewater Solids, Erik Anderson

Master's Theses (2009 -)

Phosphorus regulations are causing Water Resource Recovery Facilities (WRRFs) to implement new technologies to remove phosphorus (P) before they discharge liquid effluent. Enhanced Biological Phosphorus Removal (EBPR) is often employed to remove P from water. However, sludges from EBPR plants have shown decreases in dewaterability soon after EBPR was initiated. This decline in dewaterability is not well understood, nor is the best way to improve the dewatering EBPR sludge. Specifically, the role of different P species on sludge dewaterability is not well understood. Several laboratory experiments were conducted at the Marquette University Water Quality Center with the following objectives: i) …

Biosolids-Derived Biochar For Micropollutant Removal From Wastewater, Lee Kimbell

Master's Theses (2009 -)

Trace organic compounds including antibiotics, hormones, pharmaceuticals and personal care products are discharged to the environment with liquid and solid effluent streams from water resource recovery facilities. These compounds are referred to as micropollutants, and can have negative impacts in receiving waters. Current wastewater treatment processes are not specifically designed to remove micropollutants, and many of these compounds are recalcitrant to conventional treatment technologies. Triclosan (TCS) was selected as a representative micropollutant in this study due to frequent detection in liquid effluents, residual biosolids, and surface waters. Pyrolysis - the thermochemical decomposition of organic matter at elevated temperatures in the …

Effects Of Biochar Treatment Of Municipal Biosolids And Horse Manure On Quality Of Runoff From Fescue Plots, Rachel Williams, Dwayne R. Edwards

Biosystems and Agricultural Engineering Faculty Publications

Land-applied horse manure and municipal biosolids can increase nutrient and bacteria concentrations in runoff. Biochar has been demonstrated to have beneficial impacts on nutrient retention and runoff quality when used to treat other land-applied organic soil amendments (e.g., poultry manure). The objective of this study was to evaluate the effects of biochar addition to horse manure and municipal biosolids on runoff concentrations of nutrients and fecal coliforms. Biochar was added at 5% to 8% (wet basis) to horse manure and biosolids that were applied to 2.4 m x 6.1 m fescue plots followed by application of simulated rainfall (102 mm …

Biosolids Reduction By The Oxic-Settling-Anoxic Process: Impact Of Sludge Interchange Rate, Galilee U. Semblante, Faisal I. Hai, Heriberto A. Bustamante, Nelly Guevara, William E. Price, Long D. Nghiem

Faculty of Engineering and Information Sciences - Papers: Part A

The impact of sludge interchange rate (SIR) on sludge reduction by oxic-settling-anoxic (OSA) process was investigated. The sludge yield of an OSA system (a sequencing batch reactor, SBR, integrated with external anoxic reactors) was compared to that of a control (an SBR attached to a single-pass aerobic digester). SIR (%) is the percentage by volume of sludge returned from the external reactor into the main bioreactor of the OSA, and was varied from 0-22%. OSA achieved greater sludge reduction when fed with unsettled sewage (sCOD=113 mg/L) rather than settled sewage (sCOD=60 mg/L). The SIR of 11% resulted in the highest …

Pilot-Scale Study Of Low-Tech Methods Of Sustainable Class A Biosolids Production: A Trial Run, Rebecca L. Green

Dissertations, Master's Theses and Master's Reports

Water resource recovery facilities can improve sustainability by producing Class A biosolids. However, many conventional methods for achieving Class A biosolids are expensive, with high maintenance and energy costs. However, low-cost, low-tech (LCLT) treatment methods are also available. The overall goal of the larger project of which this study was a part is to develop a rational and universal approach for design of LCLT processes. To achieve this goal, pilot-scale studies of LCLT processes will be performed. The goal of this portion of the project was to perform a trial run pilot-scale study. During the trial run, the pilot-scale biosolids …

Emerging Investigators Series: Pyrolysis Removes Common Microconstituents Triclocarban, Triclosan, And Nonylphenol From Biosolids, J. J. Ross, Daniel Zitomer, T. R. Miller, C. A. Weirich, Patrick J. Mcnamara

Civil and Environmental Engineering Faculty Research and Publications

Reusing biosolids is vital for the sustainability of wastewater management. Pyrolysis is an anoxic thermal degradation process that can be used to convert biosolids into energy rich py-gas and py-oil, and a beneficial soil amendment, biochar. Batch biosolids pyrolysis (60 minutes) revealed that triclocarban and triclosan were removed (to below quantification limit) at 200 °C and 300 °C, respectively. Substantial removal (>90%) of nonylphenol was achieved at 300 °C as well, but 600 °C was required to remove nonylphenol to below the quantification limit. At 500 °C, the pyrolysis reaction time to remove >90% of microconstituents was less than …

Biochar From Pyrolysis Of Biosolids For Nutrient Adsorption And Turfgrass Cultivation, Daniel Elliott Carey, Patrick J. Mcnamara, Daniel Zitomer

Civil and Environmental Engineering Faculty Research and Publications

At water resource recovery facilities, nutrient removal is often required and energy recovery is an ever-increasing goal. Pyrolysis may be a sustainable process for handling wastewater biosolids because energy can be recovered in the py-gas and py-oil. Additionally, the biochar produced has value as a soil conditioner. The objective of this work was to determine if biochar could be used to adsorb ammonia from biosolids filtrate and subsequently be applied as a soil conditioner to improve grass growth. The maximum carrying capacity of base modified biochar for NH₃−N was 5.3 mg/g. Biochar containing adsorbed ammonium and potassium was …

Self-Sustaining Smouldering Combustion As A Novel Disposal Destruction Method For Waste Water Biosolids, Tarek L. Rashwan

Electronic Thesis and Dissertation Repository

Managing biosolids, the major by-product from wastewater treatment plants (WWTPs), persists as a major global challenge that often constitutes the majority of WWTP operating costs. Self-sustained smouldering is a new approach for organic waste treatment, in which the waste (i.e., the fuel) is destroyed in an energy efficient manner after mixing it with sand. Smouldering has never been applied to biosolids. Column experiments, using biosolids obtained from a WWTP, were employed to identify if - and under what conditions - smouldering could be used for treating biosolids. The parameter space in which smouldering was self-sustaining was mapped as a function …

Risk Characterization From Multipathway Exposure Associated With Land Applying Biosolids By Accounting For Multimedia Mass Loss, Karthik Kumarasamy

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

There continues to be concern among communities living in the vicinity of biosolids land application sites about potential adverse health effects from the chemical contaminants present in biosolids. This study describes a procedure that will help answer questions about the risks involved in biosolids land application at the site level, thus, this study will provide a crucial communication tool for wastewater treatment facilities and biosolids land appliers to address the concerns of the public. Specifically, the study quantifies the exposure concentrations that a human being can become exposed to during their lifetime from the practice. Crucially, it takes a quantitate …

Pyrolysis For Estrogens Removal From Wastewater Solids, Thomas C. Hoffman

Master's Theses (2009 -)

Wastewater treatment processes are not designed to remove estrogenic micropollutants and therefore when wastewater biosolids are land applied estrogenic micropollutants are discharged to the environment. Release of estrogenic compounds has deleterious effects on the terrestrial environment. Public concern stemming from micropollutants may reduce the value of biosolids which are important to water resource reclamation facilities (WRRF) as by-products. The objective of this research was to evaluate pyrolysis, the partial decomposition of organic material in an oxygen-deprived system under high temperatures, as a sustainable solution to remove estrogenic compounds from biosolids while producing a useable soil conditioner called biochar. Batch pyrolysis …

The Impact Of Triclosan On The Spread Of Antibiotic Resistance In The Environment, Daniel E. Carey, Patrick J. Mcnamara

Civil and Environmental Engineering Faculty Research and Publications

Triclosan (TCS) is a commonly used antimicrobial agent that enters wastewater treatment plants (WWTPs) and the environment. An estimated 1.1 × 10⁵ to 4.2 × 10⁵ kg of TCS are discharged from these WWTPs per year in the United States. The abundance of TCS along with its antimicrobial properties have given rise to concern regarding its impact on antibiotic resistance in the environment. The objective of this review is to assess the state of knowledge regarding the impact of TCS on multidrug resistance in environmental settings, including engineered environments such as anaerobic digesters. Pure culture studies are reviewed …

An Improved Biosolid Gasifier Model, Hannah Mclean

Electronic Theses and Dissertations

As populations increase and cities become denser, the production of waste, both sewage sludge and food biomass, increases exponentially while disposal options for these wastes are limited. Landfills have minimal space for biosolids; countries are now banning ocean disposal methods for fear of the negative environmental impacts. Agricultural application of biosolids cannot keep up with the production rates because of the accumulation of heavy metals in the soils. Gasification can convert biosolids into a renewable energy source that can reduce the amount of waste heading to the landfills and reduce our dependence on fossil fuels. A recently published chemical kinetic …

Fate Of Micropollutants During Pyrolysis Of Biosolids, John Ross

Master's Theses (2009 -)

Approximately 250 tons of organic micropollutants, including pharmaceuticals, antimicrobials, and hormones, are discharged to the environment during land application of wastewater biosolids annually. Reusing wastewater biosolids is vital to the sustainability of wastewater treatment, but current treatment processes do not remove micropollutants from biosolids in an efficient manner. Pyrolysis―the heating of biomass to temperatures between 400 and 800 °C under oxygen-free conditions―was proposed as a biosolids treatment process that could produce a beneficial soil amendment product, biochar, and remove micropollutants. The objective of this research was to determine the effect of pyrolysis temperature and residence time on the removal of …

Inactivation Of Ascaris In Double-Vault Urine-Diverting Composting Latrines In Panama: Methods And Environmental Health Engineering Field Applications, Daragh A. Gibson

USF Tampa Graduate Theses and Dissertations

The United Nations Millennium Development Goals have prioritized improving access to sanitation, but unfortunately about a third of the global population is still without an improved sanitation source and one billion still practice open defecation. Lack of access to adequate and safe sanitation means the proliferation of dangerous pathogens in the environment, especially soil-transmitted helminths (STHs). In the Bocas del Toro Province of Panama (and similar locations in the world), composting latrines have been built in many of the indigenous communities in the area. They are a form of dry or ecological sanitation and are designed to produce an end …

Fate Of Estrogens In Anaerobic Digestion And Their Removal In Advanced Oxidation, Shubhajit Sarkar

Electronic Thesis and Dissertation Repository

Estrogenic compounds are most significant endocrine disrupting compounds (EDC), which are of primary concern due to their widespread presence in the aqueous environment and also due to their adverse impact both on aquatic life and potential risk to human health. Conventional wastewater treatment plants are not designed to remove EDC, with biosorption on biosolids as the predominant removal mechanism. Anaerobic digestion is one of the most preferred processes employed for sludge stabilization in wastewater treatment plants. Advanced oxidation processes (AOPs) are seen as promising technologies for removal of estrogens from the environment. In this research, a controlled degradation study of …

Triclosan Enriches For Dehalococcoides-Like Chloroflexi In Anaerobic Soil At Environmentally Relevant Concentrations, Patrick J. Mcnamara, Mark J. Krzmarzick

Civil and Environmental Engineering Faculty Research and Publications

Triclosan is an antimicrobial agent that is discharged to soils with land-applied wastewater biosolids, is persistent under anaerobic conditions, and yet its impact on anaerobic microbial communities in soils is largely unknown. We hypothesized that triclosan enriches for Dehalococcoides-like Chloroflexi because these bacteria respire organochlorides and are likely less sensitive, relative to other bacteria, to the antimicrobial effects of triclosan. Triplicate anaerobic soil microcosms were seeded with agricultural soil, which was not previously exposed to triclosan, and were amended with 1 mg kg⁻¹ of triclosan. Triplicate control microcosms did not receive triclosan, and the experiment was run for …

Solid Waste Reduction Practices In The Southeastern United States: Public Perceptions And Proposed Policy Changes, Lauren Ashley Raup-Plummer

Masters Theses

The U.S. Environmental Protection Agency (EPA) has highlighted the municipal solid waste stream as an area of critical interest due to the non-sustainable nature of existing landfilling or “dumping” practices. In addition to traditionally defined municipal solid waste – commonly referred to as garbage or rubbish – materials such as biosolids, a by-product of wastewater treatment, and animal manure also comprise the landfilled waste stream. Currently, disposal options for biosolids waste materials are limited due to regulations imposed by the EPA to protect waterways and coastal environments, and one of the few alternatives for biosolids disposal is land application. Unfortunately, …