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Full-Text Articles in Engineering

Removal Of Trace Organic Contaminants By An Mbr Comprising A Mixed Culture Of Bacteria And White-Rot Fungi, Luong Nguyen, Faisal Hai, Shufan Yang, Jinguo Kang, Frederic Leusch, Felicity Roddick, William Price, Long Nghiem Sep 2015

Removal Of Trace Organic Contaminants By An Mbr Comprising A Mixed Culture Of Bacteria And White-Rot Fungi, Luong Nguyen, Faisal Hai, Shufan Yang, Jinguo Kang, Frederic Leusch, Felicity Roddick, William Price, Long Nghiem

Faisal I Hai

The degradation of 30 trace organic contaminants (TrOC) by a white-rot fungus-augmented membrane bioreactor (MBR) was investigated. The results show that white-rot fungal enzyme (laccase), coupled with a redox mediator (1-hydroxy benzotriazole, HBT), could degrade TrOC that are resistant to bacterial degradation (e.g. diclofenac, triclosan, naproxen and atrazine) but achieved low removal of compounds (e.g. ibuprofen, gemfibrozil and amitriptyline) that are well removed by conventional activated sludge treatment. Overall, the fungus-augmented MBR showed better TrOC removal compared to a system containing conventional activated sludge. The major role of biodegradation in removal by the MBR was noted. Continuous mediator dosing to …


Did Mineral Surface Chemistry And Toxicity Contribute To Evolution Of Microbial Extracellular Polymeric Substances?, Jie Xu, Jay M. Campbell, Nianli Zhang, William J. Hickey, Nita Sahai Apr 2014

Did Mineral Surface Chemistry And Toxicity Contribute To Evolution Of Microbial Extracellular Polymeric Substances?, Jie Xu, Jay M. Campbell, Nianli Zhang, William J. Hickey, Nita Sahai

Nita Sahai

Modern ecological niches are teeming with an astonishing diversity of microbial life in biofilms closely associated with mineral surfaces, which highlights the remarkable success of microorganisms in conquering the challenges and capitalizing on the benefits presented by the mineral-water interface. Biofilm formation capability likely evolved on early Earth because biofilms provide crucial cell survival functions. The potential toxicity of mineral surfaces toward cells and the complexities of the mineral-water-cell interface in determining the toxicity mechanisms, however, have not been fully appreciated. Here, we report a previously unrecognized role for extracellular polymeric substances (EPS), which form biofilms in shielding cells against …


Mixed Culture Electrochemically Active Biofilms And Their Microscopic And Spectroelectrochemical Studies, Mohammad Mansoob Khan Dr, S. A. Ansari, J. H. Lee, J. Lee, M. H. Cho Feb 2014

Mixed Culture Electrochemically Active Biofilms And Their Microscopic And Spectroelectrochemical Studies, Mohammad Mansoob Khan Dr, S. A. Ansari, J. H. Lee, J. Lee, M. H. Cho

Dr. Mohammad Mansoob Khan

Mixed culture electrochemically active biofilms (EABs) were developed on carbon paper using a sludge with mixed culture bacteria for microscopic and pectroelectrochemical studies because a naturally mixed culture bacterial strain is more applicable than a pure culture strain. EAB development was confirmed by microbial fuel cells (MFCs) by obtaining a constant increase in potential (∼0.36 V). Microscopic and spectroscopic studies showed that a mixed culture EABs formed on the support. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS), which are nondestructive voltammetry techniques, indicated that the EABs could be source of electrons and used effectively for …


Removal Of Trace Organic Contaminants By An Mbr Comprising A Mixed Culture Of Bacteria And White-Rot Fungi, Luong N. Nguyen, Faisal I. Hai, Shufan Yang, Jinguo Kang, Frederic D L Leusch, Felicity Roddick, William E. Price, Long D. Nghiem Oct 2013

Removal Of Trace Organic Contaminants By An Mbr Comprising A Mixed Culture Of Bacteria And White-Rot Fungi, Luong N. Nguyen, Faisal I. Hai, Shufan Yang, Jinguo Kang, Frederic D L Leusch, Felicity Roddick, William E. Price, Long D. Nghiem

William E. Price

The degradation of 30 trace organic contaminants (TrOC) by a white-rot fungus-augmented membrane bioreactor (MBR) was investigated. The results show that white-rot fungal enzyme (laccase), coupled with a redox mediator (1-hydroxy benzotriazole, HBT), could degrade TrOC that are resistant to bacterial degradation (e.g. diclofenac, triclosan, naproxen and atrazine) but achieved low removal of compounds (e.g. ibuprofen, gemfibrozil and amitriptyline) that are well removed by conventional activated sludge treatment. Overall, the fungus-augmented MBR showed better TrOC removal compared to a system containing conventional activated sludge. The major role of biodegradation in removal by the MBR was noted. Continuous mediator dosing to …


Cyclic Heating To Inhibit Bacterial Growth In Activated Carbon Point-Of-Use Treatment Devices, Irwin Silverstein, Irvine W. Wei Aug 2013

Cyclic Heating To Inhibit Bacterial Growth In Activated Carbon Point-Of-Use Treatment Devices, Irwin Silverstein, Irvine W. Wei

Irvine W. Wei

The effectiveness of using heat treatment on a cyclic basis as a means of inhibiting bacterial growth in activated carbon point-of-use treatment devices was examined. Heat treated and non-heat treated devices were compared in a controlled experiment with respect to parameters such as standard plate count, total organic carbon, total residual chlorine and head loss. The results of the study indicated that cyclic heat treatment can inhibit bacterial growth on the surface of granular activated carbon without compromising total organic carbon and total residual chlorine removal efficiencies or accelerating head loss. Microbiological testing verified that the control device had become …


Pesticide Removal By A Mixed Culture Of Bacteria And White-Rot Fungi, Faisal I. Hai, Oska Modin, Kazuo Yamamoto, Kensuke Fukushi, Fumiyuki Nakajiam, Long Nghiem Jul 2013

Pesticide Removal By A Mixed Culture Of Bacteria And White-Rot Fungi, Faisal I. Hai, Oska Modin, Kazuo Yamamoto, Kensuke Fukushi, Fumiyuki Nakajiam, Long Nghiem

Faisal I Hai

Combining activated sludge cultures with microbes harboring specific degradation pathways could constitute a relevant process for the removal of toxic and recalcitrant organic substances from wastewater. Enhanced removal of three widely used recalcitrant pesticides from their liquid mixture was demonstrated by implementing a non-acclimated mixed culture of bacteria and white-rot fungus. During an incubation period of 14 days, the mixed fungus–bacteria culture achieved 47, 98, and 62% removal of aldicarb, atrazine and alachlor from the liquid phase, respectively. This compared favorably to batches containing only non-acclimated fungus or previously published removal rates with nonacclimated bacterial cultures. Biosorption along with biodegradation …


Pesticide Removal By A Mixed Culture Of Bacteria And White-Rot Fungi, Faisal I. Hai, Oska Modin, Kazuo Yamamoto, Kensuke Fukushi, Fumiyuki Nakajiam, Long Nghiem Dec 2012

Pesticide Removal By A Mixed Culture Of Bacteria And White-Rot Fungi, Faisal I. Hai, Oska Modin, Kazuo Yamamoto, Kensuke Fukushi, Fumiyuki Nakajiam, Long Nghiem

Long D Nghiem

Combining activated sludge cultures with microbes harboring specific degradation pathways could constitute a relevant process for the removal of toxic and recalcitrant organic substances from wastewater. Enhanced removal of three widely used recalcitrant pesticides from their liquid mixture was demonstrated by implementing a non-acclimated mixed culture of bacteria and white-rot fungus. During an incubation period of 14 days, the mixed fungus–bacteria culture achieved 47, 98, and 62% removal of aldicarb, atrazine and alachlor from the liquid phase, respectively. This compared favorably to batches containing only non-acclimated fungus or previously published removal rates with nonacclimated bacterial cultures. Biosorption along with biodegradation …


Enhanced Biodegradation Of Used Engine Oil In Soil Amended With Organic Wastes, Abdul Aziz Abdul Raman May 2010

Enhanced Biodegradation Of Used Engine Oil In Soil Amended With Organic Wastes, Abdul Aziz Abdul Raman

Abdul Aziz Abdul Raman

Three organic wastes (banana skin (BS), brewery spent grain (BSG), and spent mushroom compost (SMC)) were used for bioremediation of soil spiked with used engine oil to determine the potential of these organic wastes in enhancing biodegradation of used oil in soil. The rates of biodegradation of the oil were studied for a period of 84 days under laboratory conditions. Hydrocarbon-utilizing bacterial counts were high in all the organic waste-amended soil ranging between 10.2 x 10(6) and 80.5 x 10(6) CFU/g compared to unamended control soil throughout the 84 days of study. Oil-contaminated soil amended with BSG showed the highest …