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A Multicellular Model For Differential Regulation Of Circadian Signals In The Core And Shell Regions Of The Suprachiasmatic Nucleus, Christina Vasalou, Michael A. Henson
A Multicellular Model For Differential Regulation Of Circadian Signals In The Core And Shell Regions Of The Suprachiasmatic Nucleus, Christina Vasalou, Michael A. Henson
Michael A Henson
We developed a multicellular model of the mammalian circadian clock characterized by a high degree of heterogeneity with respect to single cell periodicity and behavior (intrinsic and driven oscillators), neurotransmitter release (VIP, GABA and glutamate synthesis) and spatial organization (core and shell regions), mimicking structural patterns within the SCN associated with distinct circadian functions. We simulated the SCN core and shell separately utilizing experimentally derived connectivity schemes for the two subdivisions as observed within the rat SCN. The core was modeled via a small world network characterized by VIP and GABA colocalization, whereas the shell was simulated as a nearest …
Analysis Of Aggregate Size As A Process Variable Affecting Paclitaxel Accumulation In Taxus Suspension Cultures, Martin E. Kolewe, Michael A. Henson, Susan C. Roberts
Analysis Of Aggregate Size As A Process Variable Affecting Paclitaxel Accumulation In Taxus Suspension Cultures, Martin E. Kolewe, Michael A. Henson, Susan C. Roberts
Michael A Henson
Plant cell aggregates have long been implicated in affecting cellular metabolism in suspension culture, yet the rigorous characterization of aggregate size as a process variable and its effect on bioprocess performance has not been demonstrated. Aggregate fractionation and analysis of biomass-associated product is commonly used to assess the effect of aggregation, but we establish that this method is flawed under certain conditions and does not necessarily agree with comprehensive studies of total culture performance. Leveraging recent advances to routinely measure aggregate size distributions, we developed a simple method to manipulate aggregate size and evaluate its effect on the culture as …