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Engineering Commons

Open Access. Powered by Scholars. Published by Universities.®

2004

Cleveland State University

Membrane Science

Articles 1 - 1 of 1

Full-Text Articles in Engineering

Retention And Viability Characteristics Of Mammalian Cells In An Acoustically Driven Polymer Mesh, Zhaowei Wang, Paul Grabenstetter, Donald L. Feke, Joanne M. Belovich Jan 2004

Retention And Viability Characteristics Of Mammalian Cells In An Acoustically Driven Polymer Mesh, Zhaowei Wang, Paul Grabenstetter, Donald L. Feke, Joanne M. Belovich

Chemical & Biomedical Engineering Faculty Publications

A processing approach for the collection and retention of mammalian cells within a high porosity polyester mesh having millimeter-sized pores has been studied. Cell retention occurs via energizing the mesh with a low intensity, resonant acoustic field. The resulting acoustic field induces the interaction of cells with elements of the mesh or with each other and effectively prevents the entrainment of cells in the effluent stream. Experiments involving aqueous suspensions of polystyrene particles were used to provide benchmark data on the performance of the acoustic retention cell. Experiments using mouse hybridoma cells showed that retention densities of over 1.5 × …