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Optimization Of Temperature-Glycerol -Ph Conditions For Fed-Batch Fermentation Process For Recombinant Hookworm (Ancylostoma Caninurn) Anticoagulant Peptide (Acap-5) Production By Pichia Pastoris, Mehmet Inan, Vijay Chiruvolu, Kent M. Eskridge, George P. Vlasuk, Kenneth Dickerson, Scott Brown, Michael M. Meagher
Optimization Of Temperature-Glycerol -Ph Conditions For Fed-Batch Fermentation Process For Recombinant Hookworm (Ancylostoma Caninurn) Anticoagulant Peptide (Acap-5) Production By Pichia Pastoris, Mehmet Inan, Vijay Chiruvolu, Kent M. Eskridge, George P. Vlasuk, Kenneth Dickerson, Scott Brown, Michael M. Meagher
Papers in Biochemical Engineering
This study was undertaken to determine the optimum pH, temperature and glycerol feed rate for the production of recombinant hookworm (Ancylostoma caninum) anticoagulant peptide (rAcAP-5) by Pichia pastoris using response surface methodology (RSM). A central composite design was used as an experimental design for allocation of treatment combinations in three blocks. The variables selected for study were pH, temperature and glycerol feed rate. pH was the most important variable affecting yield, specific yield and specific activity of rAcAP-5. Glycerol feed rate had a significant effect on the specific activity of rAcAP-5 (% of total secreted protein) while temperature did not …