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Full-Text Articles in Engineering

Preliminary Evaluation Of The Effects Of A Pumping Well On Existing Surface Water Resources Located In T. 12n, R. 2e, Sec. 23, Cache And Box Elder Counties, Denny J. Johnson, Richard C. Peralta Nov 1996

Preliminary Evaluation Of The Effects Of A Pumping Well On Existing Surface Water Resources Located In T. 12n, R. 2e, Sec. 23, Cache And Box Elder Counties, Denny J. Johnson, Richard C. Peralta

Civil and Environmental Engineering Faculty Publications

This report contains the results of a preliminary study of the impact of pumping a well located in T. 12N, R. 2E, sec. 23, Cache and Box Elder Counties, Utah. A semianalytical capture zone analysis was performed to determine if pumping at the well is likely to reduce flow in Willow Creek or any of three springs in the surrounding area. The well has been used from 1974 to present and is sometimes pumped at 90 gallons per minute (gpm) (verbal communication, Veibell, 1996).


On Hack's Law, Riccardo Rigon, Ignacio Rodriguez-Iturbe, Amos Maritan, Achille Giacometti, David G. Tarboton, Andrea Rinaldo Nov 1996

On Hack's Law, Riccardo Rigon, Ignacio Rodriguez-Iturbe, Amos Maritan, Achille Giacometti, David G. Tarboton, Andrea Rinaldo

Civil and Environmental Engineering Faculty Publications

Hack's law is reviewed, emphasizing its implications for the elongation of river basins as well as its connections with their fractal characteristics. The relation between Hack's law and the internal structure of river basins is investigated experimentally through digital elevation models. It is found that Hack's exponent, elongation, and some relevant fractal characters are closely related. The self-affine character of basin boundaries is shown to be connected to the power law decay of the probability of total contributing areas at any link and to Hack's law. An explanation for Hack's law is derived from scaling arguments. From the results we …


Software For Optimizing Groundwater Or Conjunctive Water Management, Richard C. Peralta, Alaa H. Aly Jun 1996

Software For Optimizing Groundwater Or Conjunctive Water Management, Richard C. Peralta, Alaa H. Aly

Civil and Environmental Engineering Faculty Publications

US/REMAX is a computer program designed to assist water managers in developing optimal groundwater and/or surface water strategies for a wide range of management problems. It employs response matrix, regression and other methods adapted for nonlinear systems. US/REMAX performs deterministic or reliability-based, single- or multi-objective optimization. Decision variables are ground-water extraction/injection and/or surface water diversion. State variables include water flows, stages and concentrations. Hard coded objective functions and constraints are linear, nonlinear, integer or mixed integer. Special constraints can be added to address unusual situations.


Discharge Coefficient Scale Effects Analysis For Weirs, Michael Clyde Johnson May 1996

Discharge Coefficient Scale Effects Analysis For Weirs, Michael Clyde Johnson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Much work has been published regarding discharge coefficients for various weir structures. What has not been published to the same extent are the effects of model scale associated with the weirs being studied. If laboratory weirs are too small, scale effects can affect the magnitude of the discharge coefficient. These errors may be significant if the weir serves as a control structure for an emergency spillway. It is imperative that discharge be accurately predicted to enable safe design and operation.

Numerical and physical means were employed to analyze the effects of scale associated with Froude Modeling of weirs with sharp …


A Spatially-Distributed Hydrologic Model For A Small Arid Mountain Watershed, Thomas H. Jackson, David G. Tarboton, Keith R. Cooley May 1996

A Spatially-Distributed Hydrologic Model For A Small Arid Mountain Watershed, Thomas H. Jackson, David G. Tarboton, Keith R. Cooley

Civil and Environmental Engineering Faculty Publications

A distributed water balance model was developed as a part of an intensive-field study to simulate the snowmelt-driven hydrologic response of a small mountain watershed using measured values of solar radiation, wind speed, air temperature, relative humidity and precipitation as input.

Snowmelt and evapotranspiration were modeled with point energy balances, written in terms of the snow surface and soil surface temperatures, respectively, corrected for local topographic characteristics and snow drifting. Meltwater was routed to the basin outlet as topography-driven, saturated subsurface flow, with all flow in excess of local transmissivity taken as surface runoff.

The model was calibrated with 1985-6 …


Optimization Of Torquer Coil Design For Use With The Small Satellite Attitude Control Simulator, David Deloyd Anderson May 1996

Optimization Of Torquer Coil Design For Use With The Small Satellite Attitude Control Simulator, David Deloyd Anderson

Undergraduate Honors Capstone Projects

This paper presents a procedure used to optimize the performance of a ferromagnetic core magnetic torquer coil design for use on the Space Dynamics Laboratory (Logan, UT) Small Satellite Attitude Control Simulator. The items of optimization include the primary goal of maximizing the coil 's magnetic moment while reducing power consumption and system mass within given power, mass, and dimensional constraints. The optimization process makes use of several simple equations to determine a few starting points for design, after which an iterative approach based on experimentation is used to produce the final design. It is found that optimal magnetic moment …


Toward A Balanced Strategy To Address Contaminated Groundwater Plumes At The Massachusetts Military Reservation, Richard C. Peralta May 1996

Toward A Balanced Strategy To Address Contaminated Groundwater Plumes At The Massachusetts Military Reservation, Richard C. Peralta

Civil and Environmental Engineering Faculty Publications

This document contains the findings and recommendations of the Technical Review and Evaluation Team (TRET) for the plume containment project at the Massachusetts Military Reservation (MMR). The findings and recommendations are in response to the 60 Percent Plume Containment Design, submitted by Operational Technologies (OpTech) in January 1996. In short, the TRET recommends the MMR depart substantially from the strategy of simultaneous, 100 percent containment and treatment that was assigned to OpTech for design in accordance with the Record of Decision (ROD) for Interim Action. This strategy guided the course of the plume containment project over the past two years


A Nonparametric Wet/Dry Spell Model For Resampling Daily Precipitation, Upmanu L. Lall, Balaji Rajagopalan, David G. Tarboton Apr 1996

A Nonparametric Wet/Dry Spell Model For Resampling Daily Precipitation, Upmanu L. Lall, Balaji Rajagopalan, David G. Tarboton

Civil and Environmental Engineering Faculty Publications

A nonparametric wet/dry spell model is developed for resampling daily precipitation at a site. The model considers alternating sequences of wet and dry days in a given season of the year. All marginal, joint, and conditional probability densities of interest (e.g., dry spell length, wet spell length, precipitation amount, and wet spell length given prior to dry spell length) are estimated nonparametrically using at-site data and kernel probability density estimators. Procedures for the disaggregation of wet spell precipitation into daily precipitation and for the generation of synthetic sequences are proffered. An application of the model for generating synthetic precipitation traces …


Effect Of Subsurface Heterogeneity On Free-Product Recovery From Unconfined Aquifers, Jagath J. Kaluarachchi Mar 1996

Effect Of Subsurface Heterogeneity On Free-Product Recovery From Unconfined Aquifers, Jagath J. Kaluarachchi

Civil and Environmental Engineering Faculty Publications

Free-product record system designs for light-hydrocarbon-contaminated sites were investigated to evaluate the effects of subsurface heterogeneity using a vertically integrated three-phase flow model. The input stochastic variable of the areal flow analysis was the log-intrinsic permeability and it was generated using the Turning Band method. The results of a series of hypothetical field-scale simulations showed that subsurface heterogeneity has a substantial effect on free-product recovery predictions. As the heterogeneity increased, the recoverable oil volume decreased and the residual trapped oil volume increased. As the subsurface anisotropy increased, these effects together with free- and total-oil contaminated areas were further enhanced. The …


Optimal Pumping Strategies To Maximize Dissolved Tce Extraction At Mather Afb, California, Richard C. Peralta, Alaa H. Aly Mar 1996

Optimal Pumping Strategies To Maximize Dissolved Tce Extraction At Mather Afb, California, Richard C. Peralta, Alaa H. Aly

Civil and Environmental Engineering Faculty Publications

USU first estimated the future TCE concentrations that would result if no pumping strategy were implemented (Figure 5, Scenario AO). Then USU used the procedure of Appendix B with the model formulation of Appendix C to compute optimal pumping strategies. In computing optimal strategies USU cycled until an arbitrary three percent contaminant mass convergence criterion was satisfied.


Validation Of Energy Dispersive X-Ray Spectrometry As A Method To Standardize Backscattered Electron Images Of Bone, E. G. Vajda, R. D. Bloebaum, J. G. Skedros Jan 1996

Validation Of Energy Dispersive X-Ray Spectrometry As A Method To Standardize Backscattered Electron Images Of Bone, E. G. Vajda, R. D. Bloebaum, J. G. Skedros

Cells and Materials

The use of backscattered electron (BSE) imaging as a tool for the qualitative measurement of mineral content in bone has been well documented. The challenge still remains to develop BSE imaging as a tool for quantitative mineral content analysis in bone. The limiting factor has been the ability to standardize the BSE signal within and between laboratories. Energy dispersive x-ray spectrometry (EDX) has been proposed as a method to standardize the BSE operating environment. The goal of this study is to investigate the relationship between EDX-determined wt.% Ca measurements and BSE graylevels. A comparison with traditional ash content measurements will …


Laboratory Growth Systems In Biofilm Research, Julian W. T. Wimpenny Jan 1996

Laboratory Growth Systems In Biofilm Research, Julian W. T. Wimpenny

Cells and Materials

The huge variety of ecosystems that we collectively refer to as "biofilm" is reflected by the numerous different systems available to grow them in the laboratory. The relationship between in situ systems, microcosms and laboratory models is defined and discussed. The first two represent holistic approaches designed to assess the structure and function of particular biofilms: the last is analytical and reductionist, aiming to isolate specific functions of biofilms in order to understand properties that can apply to biofilm in general. Properties of a model can be completely understood whilst this is unlikely with natural ecosystems because of the possibility …


Initial Biodeterioration Of Silicone Rubber By C. Albicans And C. Aropicalis Strains Isolated From Voice Prostheses, H. C. Van Der Mei, B. Van De Belt-Gritter, F. Dijk, H. J. Busscher Jan 1996

Initial Biodeterioration Of Silicone Rubber By C. Albicans And C. Aropicalis Strains Isolated From Voice Prostheses, H. C. Van Der Mei, B. Van De Belt-Gritter, F. Dijk, H. J. Busscher

Cells and Materials

Silicone rubber voice prostheses m patients after total laryngectomy become rapidly colonized by a biofilm, consisting of yeasts and bacteria. An especially troublesome feature of these biofilms is the ingrowth into the silicone rubber by colonizing yeasts. Microcolonies of ingrowing yeasts have been frequently observed on explanted voice prostheses after 3-4 months use. Incubation of silicone rubber samples in a modified Robbins device with a culture of Candida tropicalis, isolated from a Groningen button voice prosthesis, while passing the yeasts through a cycle of feasts (7 days) and famine (also 7 days) demonstrated, by electron microscopy, the onset of the …


Investigation Of Bone And Calcium Phosphate Coatings And Crystallinity Determination Using Raman Microspectroscopy, C. J. De Grauw, J. D. De Bruijn, C. Otto, J. Greve Jan 1996

Investigation Of Bone And Calcium Phosphate Coatings And Crystallinity Determination Using Raman Microspectroscopy, C. J. De Grauw, J. D. De Bruijn, C. Otto, J. Greve

Cells and Materials

Conventional spontaneous Raman micro-spectroscopy was used for the investigation of bone and calcium phosphate coatings on bone-implant surfaces. Chemical and structural changes at the bone-coating interface could be monitored on a microscopic scale. It was shown that the crystallinity did not vary within the crystalline apatite and amorphous calcium phosphate coatings, while the density within each coating did vary. Different degrees of crystallinity in coatings were investigated for a series of plasma-sprayed apatite coatings. It is concluded that Raman microspectroscopy is an easy non-destructive way to obtain information about the apatite structure and the degree of crystallinity.


Effects Of Solidification Conditions And Heat Treatment On The Microstructure And Vickers Hardness Of Pd-Cu-Ga Dental Alloys, William A. Brantley, Zhuo Cai, Stanley G. Vermilyea, Efstratios Papazoglou, John C. Mitchell, Alan B. Carr Jan 1996

Effects Of Solidification Conditions And Heat Treatment On The Microstructure And Vickers Hardness Of Pd-Cu-Ga Dental Alloys, William A. Brantley, Zhuo Cai, Stanley G. Vermilyea, Efstratios Papazoglou, John C. Mitchell, Alan B. Carr

Cells and Materials

Two representative Pd-Cu-Ga dental alloys, one with a dendritic as-cast microstructure containing a eutectic interdendritic constituent and the other with an equiaxed fine-grained as-cast microstructure containing a near-surface eutectic constituent, have been subjected to rapid quenching following casting, in addition to the conventional bench cooling recommended by the manufacturers. The quenched alloys were subsequently heat treated at temperatures of 1200°, 1500° and 1800 op that span the range of the firing cycles for dental porcelain. Scanning electron microscopic examination showed that the lamellar eutectic constituents normally present in the microstructures of the as-cast and bench-cooled alloys persisted when the alloys …


Gap Junctional Intercellular Communication In Bone, Henry J. Donahue, Monique A. Vander Molen, Clare E. Yellowley, Zhongyong Li Jan 1996

Gap Junctional Intercellular Communication In Bone, Henry J. Donahue, Monique A. Vander Molen, Clare E. Yellowley, Zhongyong Li

Cells and Materials

Gap junctional intercellular communication has been demonstrated in bone cells and may contribute to the mechanism by which osteoblasts integrate and amplify extracellular signals, both chemical (hormonal) and biophysical (electrical and mechanical). Connexin 43 (Cx43) is the predominant gap junction protein expressed by bone cells. Experiments with osteoblastic cells in which Cx43 expression was diminished by antisense transfection demonstrate that cell-to-cell coupling in osteoblastic ROS 17/2.8 cells is via gap junctions composed of Cx43. Cellular networks of these coupling deficient clones are dramatically less responsive to parathyroid hormone (PTH) suggesting that coupling contributes to hormonal responsiveness. Furthermore, PTH per se …


Design, Modeling, And Characterization Of Fet-Seed Smart Pixel Transceiver Arrays For Optical Backplanes, David V. Plant, Alain Z. Shang, Marcos R. Otazo, David R. Rolston, Brian Robertson, Harvard Scott Hinton Jan 1996

Design, Modeling, And Characterization Of Fet-Seed Smart Pixel Transceiver Arrays For Optical Backplanes, David V. Plant, Alain Z. Shang, Marcos R. Otazo, David R. Rolston, Brian Robertson, Harvard Scott Hinton

Electrical and Computer Engineering Faculty Publications

The design, modeling, and characterization of FET-SEED smart pixel transceiver arrays fabricated for application in optical backplanes are presented. Results of digital and analog measurements on 4×4 transmitter arrays and 4×4 receiver arrays, packaged at the printed circuit-board level, will be presented. In addition, these results will be compared to device and circuit models developed for these optoelectronics. Finally, the description of the successful application of these optoelectronics to interconnect two printed circuit boards will be described.


A Hybrid-Seed Smart Pixel Array For A Four-Stage Intelligent Optical Backplane Demonstrator, David R. Rolston, David V. Plant, Ted H. Szymanski, Harvard Scott Hinton, W. S. Hsiao, Michael H. Ayliffe, David Kabal, Michael B. Venditti, P. Desai, Ashok V. Krishnamoorthy, Keith W. Goossen, J. A. Walker, B. Tseng, S. P. Hui, J. C. Cunningham, W. Y. Jan Jan 1996

A Hybrid-Seed Smart Pixel Array For A Four-Stage Intelligent Optical Backplane Demonstrator, David R. Rolston, David V. Plant, Ted H. Szymanski, Harvard Scott Hinton, W. S. Hsiao, Michael H. Ayliffe, David Kabal, Michael B. Venditti, P. Desai, Ashok V. Krishnamoorthy, Keith W. Goossen, J. A. Walker, B. Tseng, S. P. Hui, J. C. Cunningham, W. Y. Jan

Electrical and Computer Engineering Faculty Publications

This paper describes the VLSI design, layout, and testing of a Hybrid-SEED smart pixel array for a four-stage intelligent optical backplane. The Hybrid-SEED technology uses CMOS silicon circuitry with GaAs-AlGaAs multiple-quantum-well modulators and detectors. The chip has been designed based on the HyperPlane architecture and is composed of four smart pixels which act as a logical 4-bit parallel optical channel. It has the ability to recognize a 4-bit address header, inject electrical data onto the backplane, retransmit optical data, and extract optical data from the backplane. In addition, the smart pixel array can accommodate for optical inversions and bit permutations …


An Atm-Based Intelligent Optical Backplane Using Cmos-Seed Smart Pixel Arrays And Free- Space Optical Interconnect Modules, Dominic J. Goodwill, Kent E. Devenport, Harvard Scott Hinton Jan 1996

An Atm-Based Intelligent Optical Backplane Using Cmos-Seed Smart Pixel Arrays And Free- Space Optical Interconnect Modules, Dominic J. Goodwill, Kent E. Devenport, Harvard Scott Hinton

Electrical and Computer Engineering Faculty Publications

The architecture, smart pixel array chip design, and optical design of an intelligent free-space digital optical backplane for ATM switching are presented. The smart pixel chip uses reflective SEED (self-electrooptic effect device) optical modulators and detectors flip-chip bonded to CMOS circuitry. This chip is one of the most complex designs ever reported in this technology, and it operates at a simulated backplane clock rate of 125 MHz. The low-loss optical system employs f/4 diffractive minilenses and microlenses to interconnect clusters of smart pixels, and it is shown to allow 2060 connections per chip if 1-cm2 -sized smart pixel chips are …


Progress In The Smart Pixel Technologies, Harvard Scott Hinton Jan 1996

Progress In The Smart Pixel Technologies, Harvard Scott Hinton

Electrical and Computer Engineering Faculty Publications

The purpose of this paper is to review the recent progress in the developing smart pixel technologies. The paper begins by reviewing some of the rapidly evolving smart pixel terminologies. It then describes several of the smart pixel technologies that have recently emerged. Finally, it outlines the performance of these technologies in both device complexity and aggregate capacity. The reviewed SPA technologies include both the modulator-based FET-SEED, hybrid CMOS-SEED, and LCOS smart pixels and the source-based hybrid VCSEL/MSM, ELO, flip-chip-bonded VCSEL/MSM, and monolithic MSM/MESFET/VCSEL smart pixels.


Time Course Of Recruitment, Pit Formation And Apoptosis Of Osteoclast Populations On Dentin In Vitro, Jihyun Kang, Rigoberto Cornejo, Kevin J. Hirokawa, Susan M. Digiorgio, Darlene F. Howard, Susan F. Silverton Jan 1996

Time Course Of Recruitment, Pit Formation And Apoptosis Of Osteoclast Populations On Dentin In Vitro, Jihyun Kang, Rigoberto Cornejo, Kevin J. Hirokawa, Susan M. Digiorgio, Darlene F. Howard, Susan F. Silverton

Cells and Materials

The resorptive capacity of osteoclasts in vitro has been used as an indicator of bone resorption. However, the kinetics of osteoclast development and senescence is not well understood. It has been noted that mononuclear precursors migrate to calcified substrate and after multi-nucleation become competent bone resorbing cells. Osteoclasts, once finished a wave of resorption, become senescent. In order to determine the survival characteristics of osteoclast populations involved in calcified tissue destruction, we have investigated the time course of the recruitment to dentin and apoptosis of osteoclasts. We have established the linkage between these measurements and the time course of the …


Biodegradation Of A Nano-Hydroxyapatite/Collagen Composite By Peritoneal Monocyte-Macrophages, F. Z. Cui, C. Du, X. W. Su, X. D. Zhu, N. M. Zhao Jan 1996

Biodegradation Of A Nano-Hydroxyapatite/Collagen Composite By Peritoneal Monocyte-Macrophages, F. Z. Cui, C. Du, X. W. Su, X. D. Zhu, N. M. Zhao

Cells and Materials

A nano-hydroxyapatite/collagen composite was prepared by precipitation of hydroxyapatite from an aqueous solution onto collagen. Mouse peritoneal macrophages were used to investigate the in vitro biodegradation of the composite. The results showed the mechanism of phagocytosis and extracellular degradation process. The cells that belong to the mononuclear phagocyte system showed some morphological characteristics similar to those of osteoclasts and made pits on the composite surface. The local modification of the material surface by the cell was another phenomenon distinguishable from the degradation process. The degradation and modification made the material porous with a widely varying Ca/P ratio.


The Role Of Surface Characteristics In The Initial Adhesion Of Human Bone-Derived Cells On Ceramics, H. Zreiqat, O. C. Standard, T. Gengenbach, J. G. Steele, C. R. Howlett Jan 1996

The Role Of Surface Characteristics In The Initial Adhesion Of Human Bone-Derived Cells On Ceramics, H. Zreiqat, O. C. Standard, T. Gengenbach, J. G. Steele, C. R. Howlett

Cells and Materials

The tissue/biomaterial interactions of three biomaterials of potential use in bone implants were studied in vitro. The mechanism of cell adherence to various ceramic substrata has been investigated by measurement of short term (90 minutes) cell attachment and spreading rate. We have determined the effect of two serum glycoproteins, fibronectin and vitronectin, on the adhesion of human bone derived cells (HBD-cells) cultured on three different types of ceramics {alumina (Al2O3), hydroxyapatite (HAP) and yttria-doped tetragonal zirconia polycrystal (Y-TZP)} . The attachment of HBD-cells to alumina and hydroxyapatite was approximately 60% of that to Y-TZP. Furthermore, …


The Influence Of A Fibrin-Coating Inside A Biodegradable Poly(Dl-Lactide-Ε-Caprolactone) Nerve Guide On Peripheral Nerve Regeneration, W. F. A. Den Dunnen, J. M. Schakenraad, B. Van Der Lei, A. J. Pennings, P. H. Robinson Jan 1996

The Influence Of A Fibrin-Coating Inside A Biodegradable Poly(Dl-Lactide-Ε-Caprolactone) Nerve Guide On Peripheral Nerve Regeneration, W. F. A. Den Dunnen, J. M. Schakenraad, B. Van Der Lei, A. J. Pennings, P. H. Robinson

Cells and Materials

The aim of this study was to evaluate the effect of a fibrin-coating on the inner surface of a biodegradable poly(DL-lactide-E-caprolactone) nerve guide on the speed and quality of the nerve regeneration. The nerve regeneration and orientation of the nerve fibers, as well as the fibrous tissue formation were evaluated. On the short term, nerve regeneration was slightly faster in the non-coated nerve guide. After longer implantation periods (≥ 4 weeks), nerve regeneration in the fibrin-coated nerve guides was characterized by a severe inflammatory response with large numbers of macrophages and polymorphonuclear cells (PMN's). This study clearly demonstrates that nerve …


Stability Of Liposomes Prepared From Archaeobacterical Lipids And Phosphatidylcholine Mixtures, G. D. Sprott, C. J. Dicaire, L. P. Fleming, G. B. Patel Jan 1996

Stability Of Liposomes Prepared From Archaeobacterical Lipids And Phosphatidylcholine Mixtures, G. D. Sprott, C. J. Dicaire, L. P. Fleming, G. B. Patel

Cells and Materials

In vitro stabilities of liposomes prepared from the total polar lipids (TPL) of various species of Archaeobacteria were compared with those made from egg phosphatidylcholine (EPC) and from mixtures of TPLIEPC. Stability was monitored by measuring the extent of leakage of entrapped dyes. Liposomes prepared from exclusively archaeobacteriallipids (coined archaeosomes), particularly those containing the bilayer spanning tetraether lipids, were generally stable to conditions such as storage at 37°C for greater than 7 days, exposure to phospholipase A2, incubation with serum, and, to some extent, exposure to bile salts. In contrast, EPC liposomes were generally unstable to these conditions, …


Biofilm Mediated Calculus Formation In The Urinary Tract, Robert J. C. Mclean, David J. Stickler, J. Curtis Nickel Jan 1996

Biofilm Mediated Calculus Formation In The Urinary Tract, Robert J. C. Mclean, David J. Stickler, J. Curtis Nickel

Cells and Materials

Mineralization and subsequent calculus formation is a common complication of biofilm infections. In the urinary tract, these infected calculi often arise from infections by urease-producing bacteria. Ammonia, liberated by bacterial urease activity, increases urine pH, resulting in the precipitation of Ca and Mg as carbonateapatite {Ca10(PO4,CO3)6(OH,CO3)2} and struvite (NH4MgP04·6H2O). These minerals become entrapped in the organic matrix which surrounds the infecting organisms and ultimately grow into mature calculi. When the causative organisms grow on urinary catheters and stents, the resulting mineralization can …


Heterogeneity Of Natural Biofilm Communities, J. R. Lawrence, D. R. Korber, G. M. Wolfaardt Jan 1996

Heterogeneity Of Natural Biofilm Communities, J. R. Lawrence, D. R. Korber, G. M. Wolfaardt

Cells and Materials

Scanning confocal laser microscopy (SCLM) studies have shown that microbial biofilms consist of a spatially heterogeneous arrangement of bacterial cells, biogenic extracellular material, and void space. Biofilm architecture is dominated by the presence of cell aggregates interspersed by channels, the arrangement of which varies for different hydrodynamic regimes, ages, species composition, or nutrient status. This organization may regulate the flux of nutrients and oxygen, creating chemical microenvironments (Eh, pH) facilitating the growth of aerobic heterotrophs and anaerobes. The presence of microenvironments has been confirmed using laser microscopy and environmentally sensitive fluorescent probes. In addition, changes in cell number and growth …


The Effect Of Vitronectin And Other Extracellular Matrix Molecules On Endothelial Expansion And Plasminogen Activation, P. Anne Underwood, Penny A. Bean Jan 1996

The Effect Of Vitronectin And Other Extracellular Matrix Molecules On Endothelial Expansion And Plasminogen Activation, P. Anne Underwood, Penny A. Bean

Cells and Materials

Endothelial recovery following procedures used to alleviate blood vessel occlusion is modulated by the local extracellular matrix upon which it has to migrate and proliferate. This extracellular material is derived from vessel wall cells, and plasma proteins which bind to the exposed surfaces. We have demonstrated that vitronectin adsorbs efficiently to tissue culture polystyrene in competition with other plasma proteins, which suggests that it may adsorb to biomaterial surfaces in vivo. We have compared the adhesion, migration and proliferation of human umbilical artery endothelial cells on surface-coated vitronectin, with other extracellular matrix molecules encountered in this environment, namely fibronectin, …


Collagen Fibres Are Not Required For Initial Matrix Mineralization By Bone Cells, M. M. Hosseini, S. A. F. Peel, J. E. Davies Jan 1996

Collagen Fibres Are Not Required For Initial Matrix Mineralization By Bone Cells, M. M. Hosseini, S. A. F. Peel, J. E. Davies

Cells and Materials

Passaged primary cultures of young adult rat bone marrow cells were maintained in medium containing combinations of the supplements dexamethasone, ascorbic acid and Na-ß-glycerophosphate. The cultures were examined by both light and scanning electron microscopy (with additional energy dispersive X-ray analysis). In cultures with fully supplemented medium, an extracellular matrix formed at the culture dish surface, beneath developing bone nodules; this matrix was mineralized but collagen-fibre free and corresponded to that which we have reported previously as the equivalent of the cement line in vivo. In selected samples this interfacial, cement line, matrix was analyzed by X-ray …


Perturbations Of Periosteel Bone During Healing: Effect Of Non-Weight Bearing, Helen E. Gruber, Mary E. Kirchen, John R. Sweeney, William E. Anderson, G. June Marshall Jan 1996

Perturbations Of Periosteel Bone During Healing: Effect Of Non-Weight Bearing, Helen E. Gruber, Mary E. Kirchen, John R. Sweeney, William E. Anderson, G. June Marshall

Cells and Materials

Weight bearing (WB) is an important factor influencing bone remodelling. The present study evaluates the effects of weight bearing and non-weight bearing (S) (achieved by tail suspension and hindlimb elevation) on the healing of a fibular osteotomy in adult male rats. After 9, 18 or 36 days under WB or S conditions, periosteum near the callus formed at the osteotomy site was compared to periosteum of the contralateral fibula (which did not receive an osteotomy) or to periosteum of fibula of control animals which did not receive an osteotomy. Data show that periosteal bone healing is sensitive to alterations in …