Engineering Commons^™

Proteomic Analysis Of Ethene-Enriched Groundwater Microcosms From A Vinyl Chloride-Contaminated Site, Adina S. Chuang, Yang Oh Jin, Laura S. Schmidt, Yalan Li, Samuel Fogel, Donna Smoler, Timothy E. Mattes

Adina Howe

Contamination of groundwater with vinyl chloride (VC), a known human carcinogen, is a common environmental problem at plastics manufacturing, dry cleaning, and military sites. At many sites, there is the potential to cleanup VC groundwater plumes with aerobic VC-oxidizing microorganisms (e.g., methanotrophs, etheneotrophs, and VC-assimilating bacteria). Environmental biotechnologies that reveal the presence and activity of VC-oxidizing bacteria in contaminated groundwater samples would provide valuable lines of evidence that bioremediation of VC is occurring at a site. We applied targeted shotgun mass spectrometry-based proteomic methods to ethene-enriched groundwater microcosms from a VC-contaminated site. Polypeptides from the enzymes alkene monooxygenase (EtnC) and …