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Development Of Microfluidic Devices To Study Algal Chemotaxis And Long-Term Growth Dynamics, Benjamin Seth Roberts
Development Of Microfluidic Devices To Study Algal Chemotaxis And Long-Term Growth Dynamics, Benjamin Seth Roberts
LSU Master's Theses
Harmful algal blooms pose a threat to human health and the environment. Many complex factors influence their formation and development, and much is still unknown. One major influencing factor that is well-known yet poorly studied is algal chemotaxis. Many studies have examined chemotaxis in other organisms, but very little about chemotaxis in algae has been studied, largely because existing technology and assay techniques are inadequate. Microfluidics offers many possibilities for cell biology, and has been applied to the study of chemotaxis in other organisms already. These techniques can be applied to the study of algal chemotaxis as well. We present …
Microfluidic Device For Motility And Osmolality Analysis Of Zebrafish Sperm, Jacob Ethan Beckham
Microfluidic Device For Motility And Osmolality Analysis Of Zebrafish Sperm, Jacob Ethan Beckham
LSU Master's Theses
An increasing number of laboratories are evaluating sample quality via motility analysis by means of computer-assisted sperm analysis (CASA) after sperm activation by manual dilution and mixing. Even with the use of CASA, due to user variation, there is a lack of control over the activation process, resulting in inconsistent motility analysis. Low sample volume (~1-2µL), and a short motility duration (burst motility of less than 15s) add to the complexity of these difficulties. The objectives of this study were to develop a microfluidic device with the capabilities to (1) standardize the method of activation for zebrafish sperm so that …