Engineering Commons^™

Biocompatibility Of Poly-Ε-Caprolactone-Hydroxyapatite Composite On Mouse Bone Marrow-Derived Osteoblasts And Endothelial Cells, Haiying Yu, Paul H. Wooley, Shang-You Yang

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Tissue-engineered bone may be developed by seeding the cells capable of both osteogenesis and vascularization on biocompatible composite scaffolds. The current study investigated the performance of mice bone marrow-derived osteogenic cells and endothelial cells as seeded on hydroxyapatite (HA) and poly-ε-caprolactone (PCL) composite scaffolds.

Methods

Mononuclear cells were induced to osteoblasts and endothelial cells respectively, which were defined by the expression of osteocalcin, alkaline phosphatase (ALP), and deposits of calcium-containing crystal for osteoblasts, or by the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) and von Willebrand factor (vWF), and the formation of a capillary network in Matrigel™ …

The Effect Of Sodium On The Catalytic Activity Of Zno-Al2o3/Zsm-5 And Sno-Al2o3/Zsm-5 For The Transesterification Of Vegetable Oil With Methanol, Manhoe Kim, Shuli Yan, Steven O. Salley, K.Y. Simon Ng

National Biofuels Energy Laboratory

In order to elucidate the effect of sodium on the activity of ZSM-5 supported metal oxides catalysts (ZnO-Al2O3/ZSM-5 and SnO-Al2O3/ZSM-5) for the transesterification of soybean oil with methanol, ZSM-5 supported metal oxides were prepared with and without sodium hydroxide by impregnation. The metal compositions of the ZSM-5 supported metal oxide catalysts and the metal concentrations dissolved from the catalysts to the methylester phase were measured by SEM-EDS and inductive coupled plasma spectroscopy, respectively. The catalytic activity of ZnO-Al2O3/ZSM-5 and SnO-Al2O3/ZSM-5 containing sodium did not originate from surface metal oxides sites, but from surface sodium sites or dissolved sodium leached from …

Reliability Analysis Of Plank Decks, Andrzej S. Nowak, Christopher D. Eamon

Civil and Environmental Engineering Faculty Research Publications

The objective of this study is to summarize the load and resistance criteria for highway bridge plank decks, and to estimate the reliability of plank decks designed by AASHTO Code. Both transverse and longitudinal planks for a variety of typical stringer spacings and plank sizes are considered. Truck traffic load data is based on the model used to calibrate the 1994 AASHTO LRFD Code. However, for plank decks, wheel load rather than whole vehicle weight is most important, and these statistics are developed for this study. For wood planks, dead load and dynamic load are not significant. The limit state …

Detailed Characterization Of The Mouse Embryonic Stem Cell Transcriptome Reveals Novel Genes And Intergenic Splicing Associated With Pluripotency, Galih Kunarso, Kee-Yew Wong, Lawrence W. Stanton, Leonard Lipovich

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Transcriptional control of embryonic stem (ES) cell pluripotency has been a subject of intense study. Transcriptional regulators including Oct4 (Oct3/4 index), Sox2 and Nanog are fundamental for maintaining the undifferentiated state. However, the ES cell transcriptome is not limited to their targets, and exhibits considerable complexity when assayed with microarray, MPSS, cDNA/EST sequencing, and SAGE technologies. To identify novel genes associated with pluripotency, we globally searched for ES transcripts not corresponding to known genes, validated their sequences, determined their expression profiles, and employed RNAi to test their function.

Results

Gene Identification Signature (GIS) analysis, a SAGE derivative distinguished …

Reliability Of Concrete Masonry Unit Walls Subjected To Explosive Loads, Christopher D. Eamon

Civil and Environmental Engineering Faculty Research Publications

This study discuses the development of a procedure that can be used to assess the reliability of concrete masonry unit infill walls subjected to personnel-delivered blast loads. Consideration is given to maintain reasonable computational effort for both the structural analysis and reliability models. Blast load and wall resistance models are developed based on experimental and analytical data, and resistance is evaluated with a large strain, large displacement transient dynamic finite element analysis. A sensitivity analysis is conducted to identify significant random variables and a reliability analysis conducted with a feasible level of computational effort. Reliability indices are estimated for two …

Observations Of Structural Damage Caused By Hurricane Katrina On The Mississippi Gulf Coast, Christopher D. Eamon, Patrick Fitzpatrick, Dennis D. Truax

Civil and Environmental Engineering Faculty Research Publications

The loads associated with Hurricane Katrina led to the destruction or severe damage of approximately 130,000 homes and over 200 deaths in the state of Mississippi. This paper discusses the results of a field inspection of structural damage along the state’s Gulf Coast area caused by this hurricane. It was found that reinforced concrete, steel frame, and heavy timber structures generally performed well, with minimal structural damage. Precast concrete, light frame wood, and bridge structures generally performed poorly. Non-structural components of all building types, in particular facades and interior partitions subjected to storm surge, were typically destroyed. For various structures, …

Reliability-Based Optimization Of Fiber-Reinforced Polymer Composite Bridge Deck Panels, Michel D. Thompson, Christopher D. Eamon, Masoud Rais-Rohani

Civil and Environmental Engineering Faculty Research Publications

A reliability-based optimization (RBO) procedure is developed and applied to minimize the weight of eight fiber-reinforced polymer composite bridge deck panel configurations. The method utilizes interlinked finite element, optimization, and reliability analysis procedures to solve the weight minimization problem with a deterministic strength constraint and two probabilistic deflection constraints. Panels are composed of an upper face plate, lower face plate, and a grid of interior stiffeners. Different panel depths and stiffener layouts are considered. Sensitivity analyses are conducted to identify significant design and random variables. Optimization design variables are panel component ply thicknesses while random variables include load and material …

Load And Resistance Factor Calibration For Wood Bridges, Andrzej S. Nowak, Christopher D. Eamon

Civil and Environmental Engineering Faculty Research Publications

The paper presents the calibration procedure and background data for the development of design code provisions for wood bridges. The structural types considered include sawn lumber stringers, glued-laminated girders, and various wood deck types. Load and resistance parameters are treated as random variables, and therefore, the structural performance is measured in terms of the reliability index. The statistical parameters of dead load and live (traffic) load, are based on the results of previous studies. Material resistance is taken from the available test data, which includes consideration of the post-elastic response. The resistance of components and structural systems is based on …

Validation Of Lower Limb Surrogates As Injury Assessment Tools In Floor Impacts Due To Anti-Vehicular Landmine Explosions, Ana Barbir

Wayne State University Theses

The aim of this study is to assess the ability of lower limb surrogates to predict injury due to floor impact in military vehicles during anti-vehicular (AV) landmine explosions. Most such surrogates have originally been constructed for use in automotive crash applications, where footwell intrusion is the main source of lower limb injury. Land mine explosions, however, create different loading conditions on the lower leg. Comparatively, the peak loads are higher but the durations are shorter and the loading occurs axially through the tibia, instead of the axial loading combined with foot rotation that is often observed in automotive applications. …

Evaluation Of Accuracy And Efficiency Of Some Simulation And Sampling Methods In Structural Reliability Analysis, Christopher D. Eamon, Michel D. Thompson, Zhenyu Liu

Civil and Environmental Engineering Faculty Research Publications

Numerous simulation and sampling methods can be used to estimate reliability index or failure probability. Some point sampling methods require only a fraction of the computational effort of direct simulation methods. For many of these methods, however, it is not clear what trade-offs in terms of accuracy, precision, and computational effort can be expected, nor for which types of functions they are most suited. This study uses nine procedures to estimate failure probability and reliability index of approximately 200 limit state functions with characteristics common in structural reliability problems. The effects of function linearity, type of random variable distribution, variance, …

Effect Of Edge-Stiffening And Diaphragms On The Reliability Of Bridge Girders, Christopher D. Eamon, Andrzej S. Nowak

Civil and Environmental Engineering Faculty Research Publications

Secondary elements such as barriers, sidewalks, and diaphragms may affect the distribution of live load to bridge girders. The objective of this study is to evaluate their effect on girder reliability if these elements are designed to be sufficiently attached to the bridge so as not to detach under traffic live loads. Simple span, two lane structures are considered, with composite steel girders supporting a reinforced concrete deck. Several representative structures are selected, with various configurations of barriers, sidewalks and diaphragms. Bridge analysis is performed using a finite element procedure. Load and resistance parameters are treated as random variables. Random …

Modeling Concrete Masonry Walls Subjected To Explosive Loads, Christopher D. Eamon, James T. Baylot, James L. O'Daniel

Civil and Environmental Engineering Faculty Research Publications

Concrete masonry unit walls subjected to blast pressure were analyzed with the finite element method, with the goal of developing a computationally-efficient and accurate model. Wall behavior can be grouped into three modes of failure, which correspond to three ranges of blast pressures. Computational results were compared to high-speed video images and debris velocities obtained from experimental data. A parametric analysis was conducted to determine the sensitivity of computed results to critical modeling values. It was found that the model has the ability to replicate experimental results with good agreement. However, it was also found that, without knowledge of actual …

Implementation Of Viterbi Decoder On Xilinx Xc4005xl Fpga, Nabil Abu-Khader

Wayne State University Theses

The Viterbi decoding algorithm is used to decode convolutional codes and is found in many systems that receive digital data that might contain errors. The use of error-correcting codes has proven tobe an effective way to overcome data corruption in digital communication channels. In previous works, researchers describe the Viterbi Algorithm, but the accuracy does not exceed 10% of data points. Also, a lot of previous works do not follow IEEE 802.16 new specifications. Viterbi decoders are generally implemented using programmable digital signal processors (DSPs) or special purpose chip sets and application-specific integrated circuits (ASICs). Here, we aim to implement …

An Information-Theoretical Approach To The Adaptive Segmentation And Classification Of The Sleeping Electroencephalogram, Russell James Clark

Wayne State University Dissertations

Sleep is not a single homogeneous state; it consists of several identifiable states. The states are identified by the electroencephalogram (EEG), electrooculogram, and the electromyogram. The most common classification method consists of six classes labeled: wake, stage 1 through 4, and stage REM. A fixed length of data, usually 20 or 30 second epochs, are analyzed and placed in one of the six categories. An all-nigh sleep recording consists of 900 to 1500 such epochs and the task of scoring a record is tedious. Additionally, much of the detail in the EEG is lost due to the size of the …

Response Of The Lower Vertebral Column To Caudocephalad Acceleration, A. Peter Vulcan

Wayne State University Dissertations

This dissertation is concerned with the measurement of the response, in those areas of the vertebral column where fractures are most common, when the seated human is subjected to caudocephalad acceleration. Such accelerations and consequent vertebral fractures are experienced not only in ejection from aircraft, but also in survivable helicopter and aircraft crashes, and in certain types of falls. It is intended that the measurements should provide a better understanding of the mechanisms of vertebral fracture and data for the formulation of an appropriate mathematical model.