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Full-Text Articles in Engineering
Consolidated Chamber Design And Protocol For Olfactory Conditioning Assay With Drosophila Melanogaster, Sasha Bronovitskiy, Andres Castillo, Michael Yan, Fang Ju Lin
Consolidated Chamber Design And Protocol For Olfactory Conditioning Assay With Drosophila Melanogaster, Sasha Bronovitskiy, Andres Castillo, Michael Yan, Fang Ju Lin
Journal of the South Carolina Academy of Science
The olfactory conditioning assay is widely used in Alzheimer’s disease research to quantify learning and memory in Drosophila melanogaster. The assay tests ability to recall an aversive conditioned stimulus of scent paired with electrical shock when presented a choice between shock-associated and unrelated scents. The T-maze, a commonly used apparatus for olfactory conditioning assays, employs an elevator mechanism to transfer live flies from the shock-delivering training chamber to the scent selection point. This elevator mechanism is known to cause fly casualty. T-mazes are not commercially available and often difficult to reproduce. Other existing variations of olfactory conditioning apparatuses use …
Table Of Contents
Journal of the South Carolina Academy of Science
No abstract provided.
Art Or Science?, Allison Marsh
Art Or Science?, Allison Marsh
Section 3: Imaging the Fast Moving
No abstract provided.
Conclusion Panel, Allison Marsh
Mapping The Ocean Frontier, Allison Marsh
Mapping The Ocean Frontier, Allison Marsh
Section 4: Imaging the Concealed
No abstract provided.
Spotlight On Usc: South Carolina Institute For Anthropology And Archaeology, Allison Marsh
Spotlight On Usc: South Carolina Institute For Anthropology And Archaeology, Allison Marsh
Section 4: Imaging the Concealed
No abstract provided.
Seeing With Sound, Allison Marsh
Seeing With Sound, Allison Marsh
Section 4: Imaging the Concealed
No abstract provided.
World Ocean Floor, Courtesy Of The Library Of Congress, Allison Marsh
World Ocean Floor, Courtesy Of The Library Of Congress, Allison Marsh
Section 4: Imaging the Concealed
No abstract provided.
Marie Tharp At Her Drafting Table, Courtesy Of The Lamont-Doherty Earth Observatory, Earth Institute, Columbia University, Allison Marsh
Marie Tharp At Her Drafting Table, Courtesy Of The Lamont-Doherty Earth Observatory, Earth Institute, Columbia University, Allison Marsh
Section 4: Imaging the Concealed
No abstract provided.
Why Do We Collect?, Allison Marsh
Why Do We Collect?, Allison Marsh
Section 2: Imaging the Microscopic
No abstract provided.
Imaging And Aesthetics, Allison Marsh
Imaging And Aesthetics, Allison Marsh
Section 2: Imaging the Microscopic
No abstract provided.
Capturing Motion, Allison Marsh
Capturing Motion, Allison Marsh
Section 3: Imaging the Fast Moving
No abstract provided.
Understanding Fracture, Allison Marsh
Understanding Fracture, Allison Marsh
Section 3: Imaging the Fast Moving
No abstract provided.
Listen, Allison Marsh
Photographic Evidence, Allison Marsh
Photographic Evidence, Allison Marsh
Section 3: Imaging the Fast Moving
No abstract provided.
Spotlight On Usc: Mechanical Engineering, Allison Marsh
Spotlight On Usc: Mechanical Engineering, Allison Marsh
Section 3: Imaging the Fast Moving
No abstract provided.
Influencing Art, Allison Marsh
Influencing Art, Allison Marsh
Section 3: Imaging the Fast Moving
No abstract provided.
Spotlight On Usc: A.C. Moore Herbarium, Allison Marsh
Spotlight On Usc: A.C. Moore Herbarium, Allison Marsh
Section 2: Imaging the Microscopic
No abstract provided.
Observing The Minuscule, Allison Marsh
Observing The Minuscule, Allison Marsh
Section 2: Imaging the Microscopic
No abstract provided.
Structures Of Human Thymidylate Synthase R163k With Dump, Fdump And Glutathione Show Asymmetric Ligand Binding, Lydia M. Gibson, Lesa R. Celeste, Leslie L. Lovelace, Lukasz Lebioda
Structures Of Human Thymidylate Synthase R163k With Dump, Fdump And Glutathione Show Asymmetric Ligand Binding, Lydia M. Gibson, Lesa R. Celeste, Leslie L. Lovelace, Lukasz Lebioda
Faculty Publications
Thymidylate synthase (TS) is a well validated target in cancer chemotherapy. Here, a new crystal form of the R163K variant of human TS (hTS) with five subunits per asymmetric part of the unit cell, all with loop 181-197 in the active conformation, is reported. This form allows binding studies by soaking crystals in artificial mother liquors containing ligands that bind in the active site. Using this approach, crystal structures of hTS complexes with FdUMP and dUMP were obtained, indicating that this form should facilitate high-throughput analysis of hTS complexes with drug candidates. Crystal soaking experiments using oxidized glutathione revealed that …
Structure Of Human Thymidylate Synthase Under Low-Salt Conditions, Leslie L. Lovelace, Wladek Minor, Lukasz Lebioda
Structure Of Human Thymidylate Synthase Under Low-Salt Conditions, Leslie L. Lovelace, Wladek Minor, Lukasz Lebioda
Faculty Publications
Human thymidylate synthase, a target in cancer chemotherapy, was crystallized from PEG 3350 with 30 mM ammonium sulfate (AS) in the crystallization medium. The crystals are isomorphous with the high-salt crystals (~2.0 M AS) and the structure has been solved and refined (R = 22.6%, Rfree = 24.3%) at 1.8 Å resolution. The high- and low-AS-concentration structures are quite similar, with loop 181-197 is in the inactive conformation. Also, residues 95-106 and 129-135 (eukaryotic inserts region) show high mobility as assessed by poor electron density and high values of crystallographic temperature factors (residues 1-25 and 108-129 are disordered in both …