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Articles 1 - 4 of 4
Full-Text Articles in Engineering
A Synthetic Human Brain Ecm Hydrogel For Tight Control Of Astrocyte Activation, Sualyneth Galarza
A Synthetic Human Brain Ecm Hydrogel For Tight Control Of Astrocyte Activation, Sualyneth Galarza
Doctoral Dissertations
Bioengineers have aimed to design instructive extracellular matrix (ECM) models that can tailor the protein composition and biomechanics of the brain in vitro in order to study how astrocytes remodel the brain during trauma and inflammation. However, these parameters cannot be independently controlled in protein-based models, and although tunable in synthetic systems, current astrocyte cultures fail to retain their characteristic stellate morphology without becoming activated. To this date, there is no biomaterial model that can retain astrocyte quiescence in vitro. This dissertation sought to develop such an in vitro model that would enable the study of specific ECM factors …
Complementary, Semi-Automated Methods For Creating Multi-Dimensional, Peg-Based Biomaterials, Elizabeth A. Brooks, Lauren E. Jansen, Maria F. Gencoglu, Annali M. Yurkevicz, Shelly Peyton
Complementary, Semi-Automated Methods For Creating Multi-Dimensional, Peg-Based Biomaterials, Elizabeth A. Brooks, Lauren E. Jansen, Maria F. Gencoglu, Annali M. Yurkevicz, Shelly Peyton
Chemical Engineering Faculty Publication Series
Tunable biomaterials that mimic selected features of the extracellular matrix (ECM), such as its stiffness, protein composition, and dimensionality, are increasingly popular for studying how cells sense and respond to ECM cues. In the field, there exists a significant trade-off for how complex and how well these biomaterials represent the in vivo microenvironment, versus how easy they are to make and how adaptable they are to automated fabrication techniques. To address this need to integrate more complex biomaterials design with high-throughput screening approaches, we present several methods to fabricate synthetic biomaterials in 96-well plates and demonstrate that they can be …
Mimicking The Arterial Microenvironment With Peg-Pc To Investigate The Roles Of Physicochemical Stimuli In Smc Phenotype And Behavior, William G. Herrick
Mimicking The Arterial Microenvironment With Peg-Pc To Investigate The Roles Of Physicochemical Stimuli In Smc Phenotype And Behavior, William G. Herrick
Doctoral Dissertations
The goal of this dissertation was to parse the roles of physical, mechanical and chemical cues in the phenotype plasticity of smooth muscle cells (SMCs) in atherosclerosis. We first developed and characterized a novel synthetic hydrogel with desirable traits for studying mechanotransduction in vitro. This hydrogel, PEG-PC, is a co-polymer of poly(ethylene glycol) and phosphorylcholine with an incredible range of Young’s moduli (~1 kPa - 9 MPa) that enables reproduction of nearly any tissue stiffness, exceptional optical and anti-fouling properties, and support for covalent attachment of extracellular matrix (ECM) proteins. To our knowledge, this combination of mechanical range, low …
Smooth Muscle Stiffness Sensitivity Is Driven By Soluble And Insoluble Ecm Chemistry, William G. Herrick, Shruti Rattan, Thuy V. Nguyen, Michael S. Grunwald, Christopher W. Barney, Alfred J. Crosby, Shelly Peyton
Smooth Muscle Stiffness Sensitivity Is Driven By Soluble And Insoluble Ecm Chemistry, William G. Herrick, Shruti Rattan, Thuy V. Nguyen, Michael S. Grunwald, Christopher W. Barney, Alfred J. Crosby, Shelly Peyton
Chemical Engineering Faculty Publication Series
Smooth muscle cell (SMC) invasion into plaques and subsequent proliferation is a major factor in the progression of atherosclerosis. During disease progression, SMCs experience major changes in their microenvironment, such as what integrin-binding sites are exposed, the portfolio of soluble factors available, and the elasticity and modulus of the surrounding vessel wall. We have developed a hydrogel biomaterial platform to examine the combined effect of these changes on SMC phenotype. We were particularly interested in how the chemical microenvironment affected the ability of SMCs to sense and respond to modulus. To our surprise, we observed that integrin binding and soluble …