Engineering Commons^™

Interfacial Polymerization For Colorimetric Labeling Of Protein Expression In Cells, Jacob L. Lilly, Phillip R. Sheldon, Liv J. Hoversten, Gabriela Romero, Vivek Balasubramaniam, Brad J. Berron

Chemical and Materials Engineering Faculty Publications

Determining the location of rare proteins in cells typically requires the use of on-sample amplification. Antibody based recognition and enzymatic amplification is used to produce large amounts of visible label at the site of protein expression, but these techniques suffer from the presence of nonspecific reactivity in the biological sample and from poor spatial control over the label. Polymerization based amplification is a recently developed alternative means of creating an on-sample amplification for fluorescence applications, while not suffering from endogenous labels or loss of signal localization. This manuscript builds upon polymerization based amplification by developing a stable, archivable, and colorimetric …