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An Integrated Centrifugo-Opto-Microfluidic Platform For Arraying, Analysis, Identification And Manipulation Of Individual Cells, Robert Burger, D Kurzbuch, Robert Gorkin Iii, Gregor Kijanka, Macdara Glynn, C Mcdonagh, Jens Ducree
An Integrated Centrifugo-Opto-Microfluidic Platform For Arraying, Analysis, Identification And Manipulation Of Individual Cells, Robert Burger, D Kurzbuch, Robert Gorkin Iii, Gregor Kijanka, Macdara Glynn, C Mcdonagh, Jens Ducree
Robert Gorkin III
In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. We demonstrate array-based capture and imaging followed by "cherry-picking" of individual particles, first for fluorescently labelled polystyrene (PS) beads and then for cells. Different cell lines are discriminated based on intracellular as well as surface-based markers.