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Alterations In P53 And E2f-1 Function Common To Immortalized Chicken Embryo Fibroblasts, Sakthikumar Ambady, Hyunggee Kim, Seungkwon You, In-Jeong Kim, Linda Foster, James Farris, F Able Ponce Leon, Douglas Foster
Alterations In P53 And E2f-1 Function Common To Immortalized Chicken Embryo Fibroblasts, Sakthikumar Ambady, Hyunggee Kim, Seungkwon You, In-Jeong Kim, Linda Foster, James Farris, F Able Ponce Leon, Douglas Foster
Sakthikumar Ambady
A number of non-virally and non-chemically immortalized chicken embryo fibroblast (CEF) cells have been established recently in continuous cell culture. All immortal CEF cells tested showed common genetic alterations in the expression patterns of p53 and E2F-1 mRNA and protein which were down- and up-regulated, respectively. The biological effects of differentially regulated p53 and E2F-1 were determined by reporter gene transcriptional activity assays, DNA binding assays, and Northern blot analysis of the expression patterns of down-stream genes. In addition, expression of most of the cyclin genes was up-regulated in immortal CEF cells, which may be associated with the rapid cell …