Engineering Commons^™

The Development Of A Fluorescence-Based Reverse Flow Injection Analysis (Rfia) Method For Quantifying Ammonium At Nanomolar Concentrations In Oligotrophic Seawater, William Abbott

USF Tampa Graduate Theses and Dissertations

The goal of this thesis was to adopt a reverse flow injection analysis (rFIA) technique to the fluorometric analysis of the reaction o-phthaldialdehyde (OPA) with ammonium, allowing accurate measurements of ammonium concentrations lower than the detection limit of the widely used indophenol blue (IPB) colorimetric method while accounting for the background fluorescence of seawater. Ammonium is considered an essential nutrient for primary productivity, especially in the nutrient depleted surface ocean where as the most reduced form of dissolved inorganic nitrogen, it is readily assimilated via metabolic pathways. Challenges in the quantification of ammonium require more sensitive analytical techniques for …

Nutrient And Carbon-Dioxide Requirements For Large-Scale Microalgae Biofuel Production, Benjamin K. Shurtz

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ever-increasing fuel prices and a limited supply of oil worldwide are threatening our economy and way of life in both the near and distant future. In order to reduce oil
dependence, the US Department of Energy (DOE) has established a goal that by the year 2030, 30% of the US transportation fuel will be renewable. The goal equates to
approximately 60 billion gallons per year of renewable fuel.

In an effort to reach the DOE renewable fuel goal, numerous types of plants are being studied, whose oil can be harvested and refined to serve as replacement fuel.
Among the various …