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Magnetic Nanoparticles Enhanced Surface Plasmon Resonance Biosensor For Rapid Detection Of Salmonella Typhimurium In Romaine Lettuce, Devendra Bhandari, Fur-Chi Chen, Roger C. Bridgman
Magnetic Nanoparticles Enhanced Surface Plasmon Resonance Biosensor For Rapid Detection Of Salmonella Typhimurium In Romaine Lettuce, Devendra Bhandari, Fur-Chi Chen, Roger C. Bridgman
Human Sciences Faculty Research
Salmonella is one of the major foodborne pathogens responsible for many cases of illnesses, hospitalizations and deaths worldwide. Although different methods are available to timely detect Salmonella in foods, surface plasmon resonance (SPR) has the benefit of real-time detection with a high sensitivity and specificity. The purpose of this study was to develop an SPR method in conjunction with magnetic nanoparticles (MNPs) for the rapid detection of Salmonella Typhimurium. The assay utilizes a pair of well-characterized, flagellin-specific monoclonal antibodies; one is immobilized on the sensor surface and the other is coupled to the MNPs. Samples of romaine lettuce contaminated with …