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Full-Text Articles in Engineering
Gene Expression Analysis Of Immobilized Saccharomyces Cerevisiae, Ryan Michael Summers
Gene Expression Analysis Of Immobilized Saccharomyces Cerevisiae, Ryan Michael Summers
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Immobilization is an effective method to increase ethanol production, as proven by previous research. Results almost exclusively demonstrate an increase in ethanol production by and decrease in reproduction rate of immobilized Saccharomyces cerevisiae cells. Recently, research has been conducted to determine the cause of this change. The extreme variance in results due to lack of technology makes it difficult to determine the cellular changes induced by immobilization. With the advent of new technology, specifically gene expression analysis, the RNA content of cells can be easily and rapidly analyzed.
S. cerevisiae cells were immobilized in 3% (w/v) calcium alginate beads and …
Development Of A Microelectrode Array Sensing System For Water Quality Monitoring, Robert D. Gardner
Development Of A Microelectrode Array Sensing System For Water Quality Monitoring, Robert D. Gardner
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
This thesis reports the design and fabrication of a low-cost reliable microelectrode array sensing platform and its application toward water quality monitoring, including heavy metal ion detection. Individually addressable microelectrodes were designed in a planar array on a nonconductive glass substrate by a photolithography method. The size, shape, composition, and functionality of the microelectrodes were theoretically explored in order to maximize performance.
The microelectrode array sensing platform was proven and characterized in the K3Fe(CN)6 electrochemical standard using cyclic voltammetry. The sensor platform exhibited well defined voltammograms and had increased sensitivity relative to a commercially available microelectrode of similar size. …