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- Data representation (8)
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- Scientific imaging (8)
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- Biodegradation (2)
- Biomaterials and Biosystems (2)
- Corn stover (2)
- Economics (2)
- High solids (2)
- Proteomics (2)
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- 17β-estradiol (1)
- 17β-estradiol; Estrone; GAC adsorption; Biologically active carbon (1)
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- BRCA 1 (1)
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- Bacillus subtilis (1)
- Bacterial physiological phenomena (1)
- Biochemical Conversion of Acid-Pretreated Water Hyacinth (Eichhornia Crassipes) to Alcohol Using Pichia Stipitis NCIM3497 (1)
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- Section 4: Imaging the Concealed (5)
- Section 2: Imaging the Microscopic (4)
- David J. Muth (3)
- Z Li (3)
- Dartmouth Scholarship (2)
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- Kurt A. Rosentrater (2)
- Master's Theses (2)
- Weiqiang Chen (2)
- Aaron M. Hoover (1)
- Bioelectrics Publications (1)
- Biosystems and Agricultural Engineering Faculty Publications (1)
- Douglas L Karlen (1)
- IP Theory (1)
- Nebraska Center for Biotechnology: Faculty and Staff Publications (1)
- Renée Crown University Honors Thesis Projects - All (1)
- Sandip S. Magdum (1)
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Articles 1 - 30 of 32
Full-Text Articles in Engineering
Nanoroughened Surfaces For Efficient Capture Of Circulating Tumor Cells Without Using Capture Antibodies, Weiqiang Chen, Shinuo Weng, Feng Zhang, Steven Allen, Xiang Li, Liwei Bao, Raymond H. W. Lam, Jill A. Macoska, Sofia D. Merajver, Jianping Fu
Nanoroughened Surfaces For Efficient Capture Of Circulating Tumor Cells Without Using Capture Antibodies, Weiqiang Chen, Shinuo Weng, Feng Zhang, Steven Allen, Xiang Li, Liwei Bao, Raymond H. W. Lam, Jill A. Macoska, Sofia D. Merajver, Jianping Fu
Weiqiang Chen
Circulating tumor cells (CTCs) detached from both primary and metastatic lesions represent a potential alternative to invasive biopsies as a source of tumor tissue for the detection, characterization and monitoring of cancers. Here we report a simple yet effective strategy for capturing CTCs without using capture antibodies. Our method uniquely utilized the differential adhesion preference of cancer cells to nanorough surfaces when compared to normal blood cells and thus did not depend on their physical size or surface protein expression, a significant advantage as compared to other existing CTC capture techniques.
Applied And Mechanistic Studies Of Microbial 17beta-Estradiol Degradation, Zhongtian Li
Applied And Mechanistic Studies Of Microbial 17beta-Estradiol Degradation, Zhongtian Li
Z Li
The presence of natural estrogens, a class of endocrine disrupting compounds, in water has caused increasing concerns over their adverse impacts on the health of aquatic eco-systems and human beings. In this study, adsorption characteristics of two natural estrogens, 17β-estradiol (E2) and estrone (E1), on granular activated carbon (GAC) were investigated in isotherm tests and in a GAC column. The GAC column was then converted to a biologically active carbon (BAC) column and the removal efficiency of E2 and its primary biodegradation intermediate E1 were monitored. During BAC operation, the impacts of various reactor operation parameters, such as the carbon …
Inactivation Of Bacterial Opportunistic Skin Pathogens By Nonthermal Dc-Operated Afterglow Atmospheric Plasma, L. C. Heller, C. M. Edelblute, A. M. Mattson, X. Hao, J. F. Kolb
Inactivation Of Bacterial Opportunistic Skin Pathogens By Nonthermal Dc-Operated Afterglow Atmospheric Plasma, L. C. Heller, C. M. Edelblute, A. M. Mattson, X. Hao, J. F. Kolb
Bioelectrics Publications
AIMS: Multidrug-resistant opportunistic pathogens are clinically significant and require the development of new antimicrobial methods. In this study, Acinetobacter baumannii, Pseudomonas aeruginosa and Staphylococcus aureus cells were exposed to atmospheric plasma on agar plates and in vitro on porcine skin for the purpose of testing bacterial inactivation.
METHODS AND RESULTS: Microbial inactivation at varying exposure durations was tested using a nonthermal plasma jet generated with a DC voltage from ambient air. The observed reduction in colony forming units was quantified as log10 reductions.
CONCLUSIONS: Direct plasma exposure significantly inactivated seeded bacterial cells by approx. 6 log10 …
Environmental Impacts Of Stover Removal In The Corn Belt, Alicia English, Wallace E. Tyner, Juan Sesmero, Phillip Owens, David J. Muth Jr.
Environmental Impacts Of Stover Removal In The Corn Belt, Alicia English, Wallace E. Tyner, Juan Sesmero, Phillip Owens, David J. Muth Jr.
David J. Muth
When considering the market for biomass from corn stover resources erosion and soil quality issues are important to consider. Removal of stover can be beneficial in some areas, especially when coordinated with other conservation practices, such as vegetative barrier strips and cover crops. However, benefits are highly dependent on several factors, namely if farmers see costs and benefits associated with erosion and the tradeoffs with the removal of biomass. This paper uses results from an integrated RUSLE2/WEPS model to incorporate six different regime choices, covering management, harvest and conservation, into a simple profit maximization model to show these tradeoffs.
Fractionation Of Distillers Dried Grains With Solubles (Ddgs) Through A Narrowing Of Particle Size Distribution Followed By Aspiration, Rafael A. Garcia, Kurt A. Rosentrater
Fractionation Of Distillers Dried Grains With Solubles (Ddgs) Through A Narrowing Of Particle Size Distribution Followed By Aspiration, Rafael A. Garcia, Kurt A. Rosentrater
Kurt A. Rosentrater
Distiller's dried grains with solubles (DDGS) may have more value and utility if they can be separated into high protein and high fiber fractions. A variety of such separation processes have been proposed; two of the most promising processes involve three screening and three air classification unit operations. In the present study, an alternative process involving fewer unit operations is demonstrated. DDGS are subjected to a single screening, and the oversize particles are processed in a mill setup so that it narrows the particle size distribution of the oversize fraction. The milled DDGS is then processed in an air classification …
Rapid Inversion: Running Animals And Robots Swing Like A Pendulum Under Ledges, Jean-Michel Mongeau, Brian Mcrae, Ardian Jusufi, Paul Birkmeyer, Aaron M. Hoover, Ronald Fearing, Robert J. Full
Rapid Inversion: Running Animals And Robots Swing Like A Pendulum Under Ledges, Jean-Michel Mongeau, Brian Mcrae, Ardian Jusufi, Paul Birkmeyer, Aaron M. Hoover, Ronald Fearing, Robert J. Full
Aaron M. Hoover
Escaping from predators often demands that animals rapidly negotiate complex environments. The smallest animals attain relatively fast speeds with high frequency leg cycling, wing flapping or body undulations, but absolute speeds are slow compared to larger animals. Instead, small animals benefit from the advantages of enhanced maneuverability in part due to scaling. Here, we report a novel behavior in small, legged runners that may facilitate their escape by disappearance from predators. We video recorded cockroaches and geckos rapidly running up an incline toward a ledge, digitized their motion and created a simple model to generalize the behavior. Both species ran …
Ethanol And Anaerobic Conditions Reversibly Inhibit Commercial Cellulase Activity In Thermophilic Simultaneous Saccharification And Fermentation (Tssf), Kara K. Podkaminer, William R. Kenealy, Christopher D. Herring, David A. Hogsett, Lee R. Lynd
Ethanol And Anaerobic Conditions Reversibly Inhibit Commercial Cellulase Activity In Thermophilic Simultaneous Saccharification And Fermentation (Tssf), Kara K. Podkaminer, William R. Kenealy, Christopher D. Herring, David A. Hogsett, Lee R. Lynd
Dartmouth Scholarship
A previously developed mathematical model of low solids thermophilic simultaneous saccharification and fermentation (tSSF) with Avicel was unable to predict performance at high solids using a commercial cellulase preparation (Spezyme CP) and the high ethanol yield Thermoanaerobacterium saccharolyticum strain ALK2. The observed hydrolysis proceeded more slowly than predicted at solids concentrations greater than 50 g/L Avicel. Factors responsible for this inaccuracy were investigated in this study.
Enhanced Hydrocarbon Biodegradation Using Bioaugmentation With Biowish™-Aqua Fog, Michael Robert Lehrer
Enhanced Hydrocarbon Biodegradation Using Bioaugmentation With Biowish™-Aqua Fog, Michael Robert Lehrer
Master's Theses
This study was done to determine the effectiveness of a commercially available bioaugmentation product, BiOWiSHTM-Aqua FOG, for remediating petroleum-contaminated sandy soil. Biodegradation enhancement by BiOWiSHTM-Aqua FOG was evaluated in laboratory microcosms by directly measuring total petroleum hydrocarbon (TPH) and indirectly using respirometry. Attempts were made to enrich hydrocarbon-degrading bacteria in BiOWiSHTM-Aqua FOG, and the resulting enrichment cultures were screened using respirometry as well. Potential hydrocarbon-degrading bacteria in BiOWiSHTM-Aqua FOG were isolated. Experiments were performed at bench-scale using microcosm bottles containing sand contaminated with either motor oil or No. 2 diesel fuel. The …
Proteomic Analysis Of 17Β-Estradiol Degradation By Stenotrophomonas Maltophilia, Zhongtian Li
Proteomic Analysis Of 17Β-Estradiol Degradation By Stenotrophomonas Maltophilia, Zhongtian Li
Z Li
Microbial degradation plays a critical role in determining the environmental fate of steroid hormones, such as 17β-estradiol (E2). The molecular mechanisms governing the microbial transformation of E2 and its primary degradation intermediate, estrone (E1), are largely unknown. The objective of this study was to identify metabolism pathways that might be involved in microbial estrogen degradation. To achieve the objective, Stenotrophomonas maltophilia strain ZL1 was used as a model estrogen degrading bacterium and its protein expression level during E2/E1 degradation was studied using quantitative proteomics. During an E2 degradation experiment, strain ZL1 first converted E2 to E1 stoichiometrically. At 16 h …
Design And Implementation Of A Shoulder Simulator, Chelsea Lynn Kowal
Design And Implementation Of A Shoulder Simulator, Chelsea Lynn Kowal
Renée Crown University Honors Thesis Projects - All
The main objective of this thesis project is to build a shoulder simulator to be able to understand the mechanics of the shoulder, specifically the rotator cuff and deltoid muscles. The shoulder simulator should be able to mimic the important motions of the human shoulder by pulling on tendons of cadaver shoulders and moving the shoulder in various specified motions. In my thesis project, I worked on creating a shoulder simulator that would control the rotator cuff muscles along with the three deltoid muscles. In order to do this, I needed to design and test the shoulder simulator using cadaver …
The Use Of High-Solids Loadings In Biomass Pretreatment – A Review, Alicia A. Modenbach, Sue E. Nokes
The Use Of High-Solids Loadings In Biomass Pretreatment – A Review, Alicia A. Modenbach, Sue E. Nokes
Biosystems and Agricultural Engineering Faculty Publications
The use of high‐solids loadings (≥ 15% solids, w/w) in the unit operations of lignocellulose conversion processes potentially offers many advantages over lower‐solids loadings, including increased sugar and ethanol concentrations and decreased production and capital costs. Since the term lignocellulosic materials refers to a wide range of feedstocks (agricultural and forestry residues, distillery by‐products, and dedicated energy crops like grasses), the term “solids loading” here is defined by the amount of dry material that enters the process divided by the total mass of material and water added to the material. The goal of this study is to provide a consolidated …
Nanotopography Influences Adhesion, Spreading, And Self-Renewal Of Human Embryonic Stem Cells, Weiqiang Chen, Luis G. Villa-Diaz, Yubing Sun, Shinuo Weng, Jin Koo Kim, Raymond H. W. Lam, Lin Han, Rong Fan, Paul H. Krebsbach, Jianping Fu
Nanotopography Influences Adhesion, Spreading, And Self-Renewal Of Human Embryonic Stem Cells, Weiqiang Chen, Luis G. Villa-Diaz, Yubing Sun, Shinuo Weng, Jin Koo Kim, Raymond H. W. Lam, Lin Han, Rong Fan, Paul H. Krebsbach, Jianping Fu
Weiqiang Chen
Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy for accurate control and patterning of nanoroughness on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion, proliferation, clonal expansion, and self-renewal. Our results indicated that topological sensing of hESCs might include feedback regulation involving mechanosensory integrin-mediated cell matrix adhesion, myosin II, and E-cadherin. Our results also demonstrated that cellular responses to nanotopography were cell-type …
Investigation Of Bacillus Subtilis As A Biopesticide Against Botrytis Cinerea, Kenneth K. Ng
Investigation Of Bacillus Subtilis As A Biopesticide Against Botrytis Cinerea, Kenneth K. Ng
Master's Theses
The objective of this thesis was to investigate BiOWiSHTM-Aqua, a commercial dry solid formulation containing a consortium of bacteria and yeast, as a biopesticide for treatment of Botrytis cinerea, a gray mold that affects strawberries. BiOWiSHTM-Aqua was compared with another commercial product specifically used as a fungicide and bacteriocide, Serenade® Garden Disease Control Spray (concentrated Bacillus subtilis strain QST 713). Both laboratory tests as well as in vivo lab tests were conducted. BiOWiSHTM-Aqua results varied widely from plate to plate, regardless of experimental conditions. In some of these plates, inhibition zones were observed …
Removing 17Β-Estradiol From Drinking Water In A Biologically Active Carbon (Bac) Reactor Modified From A Granular Activated Carbon (Gac) Reactor, Zhongtian Li
Z Li
Estrogenic compounds in drinking water sources pose potential threats to human health. Treatment technologies are needed to effectively remove these compounds for the production of safe drinking water. In this study, GAC adsorption was first tested for its ability to remove a model estrogenic compound, 17β-estradiol (E2). Although GAC showed a relatively high adsorption capacity for E2 in isotherm experiments, it appeared to have a long mass transfer zone in a GAC column reactor, causing an early leakage of E2 in the effluent. With an influent E2 concentration of 20 μg/L, the GAC reactor was able to bring down effluent …
Mononuclear Phagocyte Intercellular Crosstalk Facilitates Transmission Of Cell-Targeted Nanoformulated Antiretroviral Drugs To Human Brain Endothelial Cells, Georgette D. Kanmogne, Sangya Singh, Upal Roy, Xinming Liu, Joellyn Mcmillan, Santhi Gorantla, Shantanu Balkundi, Nathan Smith, Yazen Alnouti, Nagsen Gautam, You Zhou, Larisa Poluektova, Alexander Kabanov, Tatiana Bronich, Howard Gendelman
Mononuclear Phagocyte Intercellular Crosstalk Facilitates Transmission Of Cell-Targeted Nanoformulated Antiretroviral Drugs To Human Brain Endothelial Cells, Georgette D. Kanmogne, Sangya Singh, Upal Roy, Xinming Liu, Joellyn Mcmillan, Santhi Gorantla, Shantanu Balkundi, Nathan Smith, Yazen Alnouti, Nagsen Gautam, You Zhou, Larisa Poluektova, Alexander Kabanov, Tatiana Bronich, Howard Gendelman
Nebraska Center for Biotechnology: Faculty and Staff Publications
Despite the successes of antiretroviral therapy (ART), HIV-associated neurocognitive disorders remain prevalent in infected people. This is due, in part, to incomplete ART penetration across the blood–brain barrier (BBB) and lymph nodes and to the establishment of viral sanctuaries within the central nervous system. In efforts to improve ART delivery, our laboratories developed a macrophage-carriage system for nanoformulated crystalline ART (nanoART) (atazanavir, ritonavir, indinavir, and efavirenz). We demonstrate that nanoART transfer from mononuclear phagocytes (MP) to human brain microvascular endothelial cells (HBMEC) can be realized through cell-to-cell contacts, which can facilitate drug passage across the BBB. Coculturing of donor MP …
Conclusion Panel, Allison Marsh
Mapping The Ocean Frontier, Allison Marsh
Mapping The Ocean Frontier, Allison Marsh
Section 4: Imaging the Concealed
No abstract provided.
Spotlight On Usc: South Carolina Institute For Anthropology And Archaeology, Allison Marsh
Spotlight On Usc: South Carolina Institute For Anthropology And Archaeology, Allison Marsh
Section 4: Imaging the Concealed
No abstract provided.
Seeing With Sound, Allison Marsh
Seeing With Sound, Allison Marsh
Section 4: Imaging the Concealed
No abstract provided.
World Ocean Floor, Courtesy Of The Library Of Congress, Allison Marsh
World Ocean Floor, Courtesy Of The Library Of Congress, Allison Marsh
Section 4: Imaging the Concealed
No abstract provided.
Marie Tharp At Her Drafting Table, Courtesy Of The Lamont-Doherty Earth Observatory, Earth Institute, Columbia University, Allison Marsh
Marie Tharp At Her Drafting Table, Courtesy Of The Lamont-Doherty Earth Observatory, Earth Institute, Columbia University, Allison Marsh
Section 4: Imaging the Concealed
No abstract provided.
Why Do We Collect?, Allison Marsh
Why Do We Collect?, Allison Marsh
Section 2: Imaging the Microscopic
No abstract provided.
Imaging And Aesthetics, Allison Marsh
Imaging And Aesthetics, Allison Marsh
Section 2: Imaging the Microscopic
No abstract provided.
Improving Biomass Logistics Cost Within Agronomic Sustainability Constratints And Biomass Quality Targets, Kevin L. Kenney, J. Richard Hess, William A. Smith, David J. Muth Jr.
Improving Biomass Logistics Cost Within Agronomic Sustainability Constratints And Biomass Quality Targets, Kevin L. Kenney, J. Richard Hess, William A. Smith, David J. Muth Jr.
David J. Muth
Equipment manufacturers have made rapid improvements in biomass harvesting and handling equipment. These improvements have increased transportation and handling efficiencies due to higher biomass densities and reduced losses. Improvements in grinder efficiencies and capacity have reduced biomass grinding costs. Biomass collection efficiencies (the ratio of biomass collected to the amount available in the field) as high as 75% for crop residues and greater than 90% for perennial energy crops have also been demonstrated. However, as collection rates increase, the fraction of entrained soil in the biomass increases, and high biomass residue removal rates can violate agronomic sustainability limits. Advancements in …
Economics Of Residue Harvest: Regional Partnership Evaluation, David W. Archer, David J. Muth Jr., Jacob J. Jacobson, Douglas L. Karlen
Economics Of Residue Harvest: Regional Partnership Evaluation, David W. Archer, David J. Muth Jr., Jacob J. Jacobson, Douglas L. Karlen
David J. Muth
Economic analyses on the viability of corn (Zea mays, L.) stover harvest for bioenergy production have largely been based on simulation modeling. While some studies have utilized field research data, most field-based analyses have included a limited number of sites and a narrow geographic distribution. An Iowa case study is developed illustrating the use of data extracted from a database of geographically distributed field studies for a region-specific economic analysis. The analysis utilizes grain and residue yield and associated management information from two Iowa field research sites that are Sun Grant Regional Partnership locations associated with the Corn Stover Regional …
Surface Roughness And Diametral Consistency Of Holes Drilled Into Ddgs/Phenolic Resin Blends, J. W. Shanafield, A. W. Otieno, R. A. Tatara, D. J. Schroeder, Kurt A. Rosentrater
Surface Roughness And Diametral Consistency Of Holes Drilled Into Ddgs/Phenolic Resin Blends, J. W. Shanafield, A. W. Otieno, R. A. Tatara, D. J. Schroeder, Kurt A. Rosentrater
Kurt A. Rosentrater
In this study, corn-based distillers dried grains with solubles (DDGS) has been utilized as a filler and blended with phenolic resin. The blends were compression molded into rectangular test specimens, into which holes have been machined using a standard 9.52 mm (3/8 in) diameter, two-fluted twist drill. A series of tests was then conducted to examine the effects of DDGS content, cutting speed, and feed rate upon the surface finish (roughness) of the interior hole slot, as well as the consistency of the drilled hole diameter. DDGS content was 0, 25, 50, and 75%, by weight. Cutting speed was 17, …
Spotlight On Usc: A.C. Moore Herbarium, Allison Marsh
Spotlight On Usc: A.C. Moore Herbarium, Allison Marsh
Section 2: Imaging the Microscopic
No abstract provided.
Observing The Minuscule, Allison Marsh
Observing The Minuscule, Allison Marsh
Section 2: Imaging the Microscopic
No abstract provided.
A Thousand Tiny Pieces: The Federal Circuit’S Fractured Myriad Ruling, Lessons To Be Learned, And The Way Forward, Jonathan R. K. Stroud
A Thousand Tiny Pieces: The Federal Circuit’S Fractured Myriad Ruling, Lessons To Be Learned, And The Way Forward, Jonathan R. K. Stroud
IP Theory
No abstract provided.
Complete Genome Sequence Of Clostridium Clariflavum Dsm 19732, Javier A. Izquierdo, Lynne Goodwin, Karen W. Davenport, Hazuki Teshima
Complete Genome Sequence Of Clostridium Clariflavum Dsm 19732, Javier A. Izquierdo, Lynne Goodwin, Karen W. Davenport, Hazuki Teshima
Dartmouth Scholarship
Clostridium clariflavum is a Cluster III Clostridium within the family Clostridiaceae isolated from thermophilic anaerobic sludge (Shiratori et al, 2009). This species is of interest because of its similarity to the model cellulolytic organism Clostridium thermocellum and for the ability of environmental isolates to break down cellulose and hemicellulose. Here we describe features of the 4,897,678 bp long genome and its annotation, consisting of 4,131 protein-coding and 98 RNA genes, for the type strain DSM 19732.