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Full-Text Articles in Engineering
Second-Harmonic Imaging Microscopy Of Living Cells, Paul J. Campagnola, Heather Clark, William A. Mohler, Aaron Lewis (Prof.), Leslie M. Loew
Second-Harmonic Imaging Microscopy Of Living Cells, Paul J. Campagnola, Heather Clark, William A. Mohler, Aaron Lewis (Prof.), Leslie M. Loew
Heather Clark
Second harmonic generation (SHG) has been developed in our laboratories as a high-resolution nonlinear optical imaging microscopy for cellular membranes and intact tissues. SHG shares many of the advantageous features for microscopy of another more established nonlinear optical technique: two-photon excited fluorescence (TPEF). Both are capable of optical sectioning to produce threedimensional images of thick specimens and both result in less photodamage to living tissue than confocal microscopy. SHG is complementary to TPEF in that it uses a different contrast mechanism and is most easily detected in the transmitted light optical path. It can be used to image membrane probes …
Lipids And Collagen Matrix Restrict The Hydraulic Permeability Within The Porous Compartment Of Adult Cortical Bone, Demin Wen, Caroline Androjna, Amit Vasanji, Joanne M. Belovich, Ronald J. Midura
Lipids And Collagen Matrix Restrict The Hydraulic Permeability Within The Porous Compartment Of Adult Cortical Bone, Demin Wen, Caroline Androjna, Amit Vasanji, Joanne M. Belovich, Ronald J. Midura
Chemical & Biomedical Engineering Faculty Publications
In vivo the hydraulic permeability of cortical bone influences the transport of nutrients, waste products and signaling molecules, thus influencing the metabolic functions of osteocytes and osteoblasts. In the current study two hypotheses were tested: the presence of (1) lipids and (2) collagen matrix in the porous compartment of cortical bone restricts its permeability. Our approach was to measure the radial permeability of adult canine cortical bone before and after extracting lipids with acetone-methanol, and before and after digesting collagen with bacterial collagenase. Our results showed that the permeability of adult canine cortical bone was below 4.0 × 10 …