Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Keyword
-
- CARPT (2)
- CT (2)
- Chicken; Tumour necrosis-like factor; Macrophages; Biological activities (2)
- Chromatography (2)
- In vitro (2)
-
- Interferon-y (2)
- Nitric oxlde (2)
- 5-bisphosphate carboxylase/oxygenase (Rubisco). (1)
- Amino-protected glutamic acid; biodegradation; epoxy; lipase; polyesters (1)
- Bovine oocytes (1)
- Bubble column (1)
- Bubble columns (1)
- CFD (1)
- Chlamydomonas reinhardtii — CO2-concentrating mechanism (CCM) — Glycolate excretion — Phosphoglycolate phosphatase — Photorespiration — Ribulose- l (1)
- Diffusion in solids (1)
- Discrete cell model (1)
- Dissolution (1)
- Eddy diffusivities (1)
- Flow visualization (1)
- Green fluorescent protein (1)
- High pressure trickle bed reactors (1)
- Hydrogenation (1)
- In vitro fertilization (1)
- Induced flow modulation (1)
- Internals (1)
- Liquid holdup (1)
- Magnetic resonance imaging (1)
- Measurement techniques (1)
- Microstructure (1)
- Minimization of energy dissipation rate (1)
- Publication
- Publication Type
Articles 1 - 13 of 13
Full-Text Articles in Engineering
Bioactivities Of A Tumour Necrosis Like Factor Released By Chicken Rnacrophages, Silke Rautenschlein, Anuradha Subramanian, Jagdev M. Sharma
Bioactivities Of A Tumour Necrosis Like Factor Released By Chicken Rnacrophages, Silke Rautenschlein, Anuradha Subramanian, Jagdev M. Sharma
Papers in Biotechnology
To test for tumour necrosis-like factor (TNF) of chickens, supernatants of a lipopolysaccharide (LPS)-stimulated chicken macrophage cell line MQ-NCSU were analysed. A sequence of ion-exchange and gel-permeation chromatography was utilised to isolate TNF-like activity from the culture supernatant. The peak of TNF-like cytotoxic activity corresponded to the fractions with a nlolecular weight of 81 kDa or higher. Polyclonal anti-human TNF-a antiserum cross-reacted by Western blotting with a 17 kDa protein in the TNF-containing fraction ~ ~ n d e denaturing conditions. This result indicated that chicken TNF-like factor in the biologically active form may be a protein multimer of monomers …
Bioactivities Of A Tumour Necrosis Like Factor Released By Chicken Macrophages, Silke Rautenschlein, Anuradha Subramanian, Jagdev M. Sharma
Bioactivities Of A Tumour Necrosis Like Factor Released By Chicken Macrophages, Silke Rautenschlein, Anuradha Subramanian, Jagdev M. Sharma
Papers in Biotechnology
To test for tumour necrosis-like factor (TNF) of chickens, supernatants of a lipopolysaccharide (LPS)-stimulated chicken macrophage cell line MQ-NCSU were analysed. A sequence of ion-exchange and gel-permeation chromatography was utilised to isolate TNF-like activity from the culture supernatant. The peak of TNF-like cytotoxic activity corresponded to the fractions with a nlolecular weight of 81 kDa or higher. Polyclonal anti-human TNF-a antiserum cross-reacted by Western blotting with a 17 kDa protein in the TNF-containing fraction ~ ~ n d e denaturing conditions. This result indicated that chicken TNF-like factor in the biologically active form may be a protein multimer of monomers …
Fluid Dynamic Parameters In Bubble Columns With Internals, Jinwen Chen, Fan Li, Sujatha Degaleesan, Puneet Gupta, Muthanna H. Al-Dahhan, Milorad P. Duduković, Bernard A. Toseland
Fluid Dynamic Parameters In Bubble Columns With Internals, Jinwen Chen, Fan Li, Sujatha Degaleesan, Puneet Gupta, Muthanna H. Al-Dahhan, Milorad P. Duduković, Bernard A. Toseland
Chemical and Biochemical Engineering Faculty Research & Creative Works
The Knowledge of Gas Holdup, Liquid Recirculation and Turbulent Parameters is Important for Design and Performance Calculation of Bubble Column Reactors. Although Numerous Experimental Studies Have Been Reported on This Subject, Most Are Point Measurements Limited to Columns Without Internals Operated at Low Gas Velocities. in This Study, We Present the Results Obtained for the Gas Holdup Profiles, Time-Averaged Liquid Velocity Profiles, Turbulent Stresses and Eddy Diffusivities (Radial and Axial) Obtained in a 18″ (44 Cm) Diameter Column Without and with Internals Similar to Those Used in Industrial Scale Units (E.g., Heat Exchanger Tubes) using Both Air/water and Air/drakeoil 10 …
Two-Phase Flow Distribution In 2d Trickle-Bed Reactors, Y. Jiang, M. R. Khadilkar, M. (Muthanna) H. Al-Dahhan, M. P. Dudukovic
Two-Phase Flow Distribution In 2d Trickle-Bed Reactors, Y. Jiang, M. R. Khadilkar, M. (Muthanna) H. Al-Dahhan, M. P. Dudukovic
Chemical and Biochemical Engineering Faculty Research & Creative Works
An Extended Discrete Cell Model (DCM), based on Minimization of Energy Dissipation Rate, is Applied to Predict Two-Phase Flow Distribution in the Two-Dimensional Trickle-Bed Reactors. the Main Advantages of DCM Are that It Can Qualitatively Capture the Experimental Observations, and Readily Distinguish between Flow Distribution in Prewetted and Non-Prewetted Beds, as Well as Reflect the Effects of Bed Structure and Inlet Liquid Distributor on Two Phase Flow Distribution. for Comparison Purpose, the Results of Liquid Distribution Obtained by DCM Are Compared with Both Computational Fluid Dynamics (CFD) Simulations and Experimental Observations in a 2D Bed. the Achieved Qualitative and Quantitative …
Comparative Hydrodynamics Study In A Bubble Column Using Computer-Automated Radioactive Particle Tracking (Carpt)/Computed Tomography (Ct) And Particle Image Velocimetry (Piv), Jinwen Chen, Abdenour Kemoun, Muthanna H. Al-Dahhan, Milorad P. Duduković, D. J. Lee, Liang Shih Fan
Comparative Hydrodynamics Study In A Bubble Column Using Computer-Automated Radioactive Particle Tracking (Carpt)/Computed Tomography (Ct) And Particle Image Velocimetry (Piv), Jinwen Chen, Abdenour Kemoun, Muthanna H. Al-Dahhan, Milorad P. Duduković, D. J. Lee, Liang Shih Fan
Chemical and Biochemical Engineering Faculty Research & Creative Works
The Hydrodynamics of a 10-Cm-Diameter Cylindrical Bubble Column at the Superficial Gas Velocity of 2, 4, and 8 Cm/s Are Investigated by Computer-Automated Radioactive Particle Tracking (CARPT), Particle Image Velocimetry (PIV), and Computed Tomography (CT). These Experimental Techniques Are Capable of Providing the Knowledge of Velocity and Holdup Fields in a Bubble Column System, Which Are Essential as the Experimental Benchmark for Modeling of Such Systems. the Flow Field of Liquid Phase, as Well as the Reynolds Stresses, Obtained by CARPT and PIV in an Air-Water System Are Compared in Detail. the Results Indicate that CARPT and PIV Complement Each …
Parametric Study Of Unsteady-State Flow Modulation In Trickle-Bed Reactors, M. R. Khadilkar, M. (Muthanna) H. Al-Dahhan, M. P. Duduković
Parametric Study Of Unsteady-State Flow Modulation In Trickle-Bed Reactors, M. R. Khadilkar, M. (Muthanna) H. Al-Dahhan, M. P. Duduković
Chemical and Biochemical Engineering Faculty Research & Creative Works
Unsteady-State Liquid Flow Modulation (Periodic Operation) Was Investigated for Hydrogenation of Alpha-Methylstyrene to Cumene in a Hexane Solvent over 0.5% Pd on Alumina Spheres. This Test Reaction Was Run under Both Gas and Liquid Reactant-Limited Conditions. It is Shown that Periodic Liquid Flow Modulation Can Alter the Supply of Liquid and Gaseous Reactants to the Catalyst and Result in Reactor Performance Different from that Obtained under Steady-State Conditions. the Effect of Key Parameters Such as Extent of Gas/liquid Limitation, Total Cycle Period, Cycle Split, and Liquid Mass Velocity Were Investigated Experimentally to Demonstrate the Cause-Effect Relationships in Periodic Operation. Performance …
Biodegradation Of Thermoplastic And Hermosetting Polyesters From Z-Protected Glutamic Acid, R M. Tadros, Hossein Noureddini, Delmar C. Timm
Biodegradation Of Thermoplastic And Hermosetting Polyesters From Z-Protected Glutamic Acid, R M. Tadros, Hossein Noureddini, Delmar C. Timm
Papers in Biomolecular Engineering
In a previous article,1 the development and molecular characterization of three polyesters from N-carbobenzyloxy-L-glutamic acid (ZGluOH) were reported. The polymers were a linear, heterochain polyester (ZGluOH and ethylene glycol), a crosslinked heterochain polyester (ZGluOH and diglycidyl ether of 1,4-butanediol), and a crosslinked, heterochain aromatic polyester (ZGluOH and diglycidyl ether of bisphenol A). In this manuscript, results of biodegradation studies are reported. The three polymers hydrolyzed to low molecular weight oligomers similar to the monomers with lipase. When exposed to a mixed culture of micro-organisms, the first two resins degraded to biomass and respiratory gases. The crosslinked heterochain aromatic polyester resisted …
A Mutant Of Chlamydomonas Reinhardtii With Reduced Rate Of Photorespiration, Kensaku Suzuki, Tarlan G. Mamedov, Toraoyoshi Ikawa
A Mutant Of Chlamydomonas Reinhardtii With Reduced Rate Of Photorespiration, Kensaku Suzuki, Tarlan G. Mamedov, Toraoyoshi Ikawa
Papers in Biochemical Engineering
Photorespiration rates under air-equilibrated conditions (0.04% CO2 and 21% O2) were measured in Chlamydomonas reinhardtii wild-type 2137, a phosphoglycolate- phosphatase-deficient (pgpl) mutant and a suppressor double mutant (7FR2N) derived from the pgpl mutant. In both cells grown under 5% CO2 and adapted air for 24 h in the suppressor double mutant, the maximal rate of photorespiration (phosphoglycolate synthesis) was only about half of that in either the wild type or the pgpl mutant (18-7F) cells. In the progeny, the reduced rate of photorespiration was accompanied by increased photosynthetic affinity for inorganic carbon and the capacity for growth under air whether …
Bovine Follicular Dynamics, Oocyte Recovery,And Development Of Oocytes Microinjected With A Green Fluorescent Protein Construct, M S. Chauhan, S Nadir, T L. Bailey, A W. Pryor, S P. Butler, D R. Notter, William H. Velander, F C. Gwazdauskas
Bovine Follicular Dynamics, Oocyte Recovery,And Development Of Oocytes Microinjected With A Green Fluorescent Protein Construct, M S. Chauhan, S Nadir, T L. Bailey, A W. Pryor, S P. Butler, D R. Notter, William H. Velander, F C. Gwazdauskas
Papers in Biotechnology
The present study was carried out to 1) evaluate the viability of in vitro fertilized zygotes after microinjection of DNA, 2) assess the influence of oocyte quality upon the development rate of embryos when injected with DNA, and 3) determine the integration frequency of green fluorescent protein DNA into microinjected embryos. Oocytes were aspirated from ovaries of nine nonlactating Holsteins and were categorized into grades A, B, C, and D. At 16 h after in vitro fertilization, approximately half of the pronuclear stage presumptive zygotes were classified as having 1 pronucleus or 2 pronuclei, and they were microinjected with DNA …
Magnetic Resonance Imaging Analysis Of Molecular Mobility During Dissolution Of Poly(Vinyl Alcohol) In Water, Balaji Narasimhan, J. E.M. Snaar, R. W. Bowtell, S. Morgan, C. D. Melia, N. A. Peppas
Magnetic Resonance Imaging Analysis Of Molecular Mobility During Dissolution Of Poly(Vinyl Alcohol) In Water, Balaji Narasimhan, J. E.M. Snaar, R. W. Bowtell, S. Morgan, C. D. Melia, N. A. Peppas
Balaji Narasimhan
Magnetic resonance imaging is used to study changing microstructure and molecular motion during dissolution of poly(vinyl alcohol) (PVA) in water. One-dimensional water concentration profiles were measured as a function of distance from the polymer-solvent interface. Diffusion-weighted profiles were used to calculate the spatial variation of the self-diffusion coefficient of water. The results indicate that diffusion coefficient values decrease toward the glassy core of the polymer. Self-diffusion coefficient values within the dissolving polymer increase with increasing dissolution time, while those near the polymer-solvent interface remain fairly constant. The effect of PVA molecular weight on the dissolution mechanism was investigated, with MÌ„n …
A Red-Dot-Blot Protein Assay Technique In The Low Nanogram Range, Tiilin Marcol, Anuradha Subramanian
A Red-Dot-Blot Protein Assay Technique In The Low Nanogram Range, Tiilin Marcol, Anuradha Subramanian
Papers in Biotechnology
A simple, sensitive, rapid (3 min), and highly reproducible solid-phase assay for the detection of proteins in the low nanogram range (4 ng) is described. The assay is based on differential Pon-ceau S staining of the protein spots on nitrocellulose and quantification of the protein- dye com-plexes on lubricated membranes using a densitometer. The dye solution used for protein stain-ing contained 0.1% Ponceau S in 15% phosphoric acid and 10% ethanol. Proteins were directly spotted onto pre- Ponceau S-stained nitrocellulose membranes, crosslinked with glutaraldehyde, rinsed in NaOH, restained with Ponceau S, and finished by rinsing in acid water at pH …
A Red-Dot-Blot Protein Assay Technique In The Low Nanogram Range, Tiilin Marcol, Anuradha Subramanian
A Red-Dot-Blot Protein Assay Technique In The Low Nanogram Range, Tiilin Marcol, Anuradha Subramanian
Papers in Biotechnology
A simple, sensitive, rapid (3 min), and highly reproducible solid-phase assay for the detection of proteins in the low nanogram range (4 ng) is described. The assay is based on differential Pon-ceau S staining of the protein spots on nitrocellulose and quantification of the protein- dye com-plexes on lubricated membranes using a densitometer. The dye solution used for protein stain-ing contained 0.1% Ponceau S in 15% phosphoric acid and 10% ethanol. Proteins were directly spotted onto pre- Ponceau S-stained nitrocellulose membranes, crosslinked with glutaraldehyde, rinsed in NaOH, restained with Ponceau S, and finished by rinsing in acid water at pH …
Liquid Holdup Measurement Techniques In Laboratory High Pressure Trickle Bed Reactors, Muthanna H. Al-Dahhan, Wes Highfill
Liquid Holdup Measurement Techniques In Laboratory High Pressure Trickle Bed Reactors, Muthanna H. Al-Dahhan, Wes Highfill
Chemical and Biochemical Engineering Faculty Research & Creative Works
Three Different Techniques, Which Are Tracer, Drainage and Weighing Methods, Are Used to Measure Liquid Holdup in a Laboratory Trickle-Bed Reactor Operated under High Pressure. the Holdup Measurements Are Compared to Determine the Applicability of These Methods at High Pressure Operation. It Was Found that Tracer and Drainage Techniques Give Comparable Values for the Total Liquid Holdup. Although Several Investigators Recommended the Weighing Method over the Others based on their Experiments Performed at Atmospheric Conditions, It Was Found that the Weighing Method Failed to Measure Liquid Holdup Properly at High Pressure Operation.