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Improved Functional Prediction Of Hypothetical Proteins From Listeria Monocytogenes 08-5578, Michael J. Lagier, Brittany Bowman, Kelsey Brend, Katherine Hobbs, Michael Foggia, Mark Mcdaniel

Journal of the Iowa Academy of Science: JIAS

Listeria monocytogenes is a foodborne human pathogen responsible for listerosis. The genomes of several L. monocytogenes strains have been recently sequenced. The genome of L. monocytogenes 08-5578, which was in part responsible for a significant listerosis outbreak in 2008, contains an unexpectedly high percentage of protein-encoding genes (1,927 out of 3,161; 60.96%) autonomously annotated as hypothetical proteins. The aim of this study was to test whether a manual annotation strategy could be used to assign more meaningful functional names to the hypothetical proteins of 08-5578. A holistic, manual gene annotation strategy that utilized sequence homology, cellular localization predictions, structure-based evidence, …