Open Access. Powered by Scholars. Published by Universities.®
Science and Mathematics Education Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Science and Mathematics Education
Improved Functional Prediction Of Hypothetical Proteins From Listeria Monocytogenes 08-5578, Michael J. Lagier, Brittany Bowman, Kelsey Brend, Katherine Hobbs, Michael Foggia, Mark Mcdaniel
Improved Functional Prediction Of Hypothetical Proteins From Listeria Monocytogenes 08-5578, Michael J. Lagier, Brittany Bowman, Kelsey Brend, Katherine Hobbs, Michael Foggia, Mark Mcdaniel
Journal of the Iowa Academy of Science: JIAS
Listeria monocytogenes is a foodborne human pathogen responsible for listerosis. The genomes of several L. monocytogenes strains have been recently sequenced. The genome of L. monocytogenes 08-5578, which was in part responsible for a significant listerosis outbreak in 2008, contains an unexpectedly high percentage of protein-encoding genes (1,927 out of 3,161; 60.96%) autonomously annotated as hypothetical proteins. The aim of this study was to test whether a manual annotation strategy could be used to assign more meaningful functional names to the hypothetical proteins of 08-5578. A holistic, manual gene annotation strategy that utilized sequence homology, cellular localization predictions, structure-based evidence, …