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Seasonal Distribution And The Retention Of Some Decapod Crustacean Larvae Within The Chesapeake Bay, Virginia, Joseph William Goy

OES Theses and Dissertations

Planktonic stages or 45 genera and species or decapod larvae were identified and staged from samples taken over a three year period in the lower portion and entrance area or Chesapeake Bay. The distribution of each larval stage of the decapod crustaceans found in the bay was determined and from this, the extent of the respective breeding season was deduced. In 1975, samples were taken monthly from stations along a transect at the baymouth, both at the surface and bottom. Two stations were also occupied over the tidal cycle in daylight and darkness. Evidence is presented that larvae of some …

Effect Of The Chesapeake-Elizabeth Sewage Outfall Virginia Beach, Virginia On The Distribution And Ecology Of Benthic Foraminifera, James M. Bates Jr.

OES Theses and Dissertations

Sediment and bottom water samples from seven stations at the Chesapeake Elizabeth sewage outfall, Virginia Beach, Virginia were collected in order to evaluate the effect of the outfall on benthic foraminifera. The top centimeter of sediment was analyzed for living and total (live plus dead) foraminiferal densities, chlorophyll a, sediment grain size, ATP, percentage of silt and clay, and total organic carbon. Bottom water salinity, temperature, and ammonia were also analyzed.

Living foraminiferal density increased from March to July and with distance from the outfall. Total foraminiferal density also increased from March to July. The set of environmental variables was …

The Effects Of Dredging On The Stability Of An Ebb-Tidal Delta, Lynnhaven Inlet, Virginia, Donald Keith Riggenbach

OES Theses and Dissertations

A channel was dredged in 1965 through the ebb-tidal delta of Lynnhaven Inlet, Virginia. The purpose of this study was to determine if this channel has affected the morphological configuration of the delta. It was proposed that the channel caused the dynamically stable delta to become unstable and change its morphology.

Bathymetric comparisons of seven surveys during the last 122 years, including one conducted during this investigation, show that prior to 1962 and the dredging operations, the delta had become dynamically stable. The present survey shows that the delta has changed its shape in response to the interaction of the …

A Comparative Study Of A Salt Water Impoundment With Its Adjacent Tidal Creek Pertinent To Culture Of Crassostrea Virginica (Gmelin), William D. Anderson Iii

OES Theses and Dissertations

Certain physical, chemical and biological characteristics associated with the culture of subtidal Crassostrea virginica were assessed in a salt water impoundment and its adjacent tidal feeder creek. Large seed oysters (initial y = 57.3 mm) were transferred from a somewhat polluted estuary of Charleston Harbor to floating, mid-water, and bottom hardware cloth trays (200/tray) in a four hectare pond. Identical trays at comparable depths were placed in the tidal creek and each location was sampled monthly for growth and survival. Surface and bottom water samples were collected weekly throughout the study and hourly during four seasonal 25 hour stations. Standard …

The Pathophysiology Of Acute Venous Occlusion, Lewis H. Curd Jr.

Biological Sciences Theses & Dissertations

Large artery, pedal small vein, femoral vein and central venous pressures and extremity volume changes were recorded in mongrel dogs in which the hindlimb was perfused at a constant flow. Arterial, pedal venous and femoral venous blood gases were drawn and analyzed for measurements of arterio-venous O₂ and CO₂ differences. Paw and thigh resistances were calculated. Measurements were repeated following ligation of the iliac and femoral veins, alpha receptor blockade (dibenzyline), and dilation with Vasodilan. Results indicate that ligation of extremity veins results in an active ·increase in total limb resistance below the ligation, an increase in the …

Stability And Local Effects Of An Offshore Sand Storage Mound, Dam Neck Disposal Site, Virginia Inner Continental Shelf, William Joslyn Saumsiegle

OES Theses and Dissertations

From 1967 to 1974 medium to coarse sand dredged from the Chesapeake Bay was stockpiled approximately three miles offshore of Dam Neck, Virginia in hopes that the sand could be recovered for local beach renourishment projects. The resulting mound was surveyed over a two year period to determine if the sand is being removed by wave and current action and if the creation of a new feature on the ocean bottom changes the wave refraction patterns and perhaps creates local erosion.

Bathymetric profiles across the storage mound show some apparent morphological changes, but a detailed analysis of this data is …

The Effect Of 4-Chlororesorcinol On The Growth Potential Of Dunaliella Tertiolecta Butcher (Dun Clone), John Anthony Chigges

OES Theses and Dissertations

Jolley (1973) identified and quantified 4-chlororesorcinol at the 1.2 μg/1 level in secondary treated sewage effluent as one of 17 stable organochlorine compounds. The present report presents the first data on the effect of 4-chlororesorcinol on the growth potential of the marine phytoplankter, Dunaliella tertiolecta Butcher (Dun clone).

The assay method used herein was that of the Marine Algal Assay Procedure: Bottle Test (EPA, 1974). An algal culture chamber was constructed capable of providing controlled temperature (18 ± 2°C) and light (400-500 ft-c) conditions. Four parameters were used to describe the growth response of D. tertiolecta including maximum specific growth …

Numerical Simulation Of Thermohaline Convection In The Mixed Layer Of The Ocean, Victor Eli Delnore

OES Theses and Dissertations

The intradiumal heating and cooling cycle of the mixed layer of a tropical ocean is investigated through the use of a pseudo-two-dimensional numerical model. Particular emphasis is given to two-component diffusion resulting from dynamic instabilities in the water column. The conservation equations for salt and heat include the effects of solar heating, horizontal advectlon, and turbulent fluxes at the sea surface, while wind mixing enter through the use of depth-dependent eddy diffusion coefficients resulting from the wave-orbital shear model of KitaigorodsldLy. All inputs are treated as functions of time-of-day, or calculated via the bulk aerodynamic method.

The entrainment fluxes of …

Structural Comparisons Of Four Plant Communities In The Great Dismal Swamp, Claire Virginia Dabel

Biological Sciences Theses & Dissertations

Four plant communities in the Great Dismal Swamp, located on the coastal plain in southeastern Virginia, were sampled and estimates of density, basal area and aboveground standing crop biomass were determined, Biomass was estimated as 229,800 kg/ha in the Chamaecyparis thyoides community and as 344,500 kg/ha in the Taxodium distichum community. Biomass for the two hardwood communities, the

Acer-Nyssa community and mixed hardwood (Quercus-Nyssa-Acer-Liriodendron-Liquidamber) community was estimated as 195,700 kg/ha and 194,600 kg/ha respectively and did not differ significantly (P

An Investigation Of The Nucleotide Cyclic 3'5' Adenosine Monophosphate In Young Hypocotyls Of Pisum Sativum L., Gary Marsh Hilton

Biological Sciences Theses & Dissertations

Concentrations of cAMP increased with time in pea hypocotyls assayed at 24 hour intervals over a period of four days. The highest concentration was 20.1 pmoles/mg protein which occurred in 96 hour hypocotyl tips. Ninety-six hour root bodies (dissected region between tip and embryo) achieved a concentration of 8.6 pmoles/mg. Concentrations of cAMP in embryos remained constant around one pmole/mg for three days and values doubled on the fourth day.

Hypocotyls, after germination for 72 hours, were dissected and incubated for various lengths of time in water and 3xl0^-8M IAA. Tips incubated for two hours showed a tenfold …

An Intensive Study Of The Spring Phytoplankton Bloom In Lynnhaven Bay, Virginia, Donald Lynn Webster

Biological Sciences Theses & Dissertations

Phytoplankton samples were collected biweekly from two stations, one in each branch of Lynnhaven Bay, Virginia over the period January 24 to June 9, 1975. Water temperature, salinity, pH, oxygen, ammonia, urea, reactive nitrite, reactive nitrate, reactive phosphorus, productivity, transparency, and weather conditions were recorded concurrently. Data were manipulated employing analysis of variance, simple correlation, correlation matrices, and forward stepwise multiple regression. Total phytoplankton numbers exhibited a unimodal pattern. A pre-bloom pulse was recorded at both stations in late February. The spring bloom lasted from April through May. Phytoplankton growth was primarily a function of nutrient supply and temperature. Nutrient …

Studies Of The Purification Of Nad-Dependent And Nadp-Dependent Isocitrate Dehydrogenase By General Ligand Affinity Chromatography, Geoffrey H. Bertkau

Chemistry & Biochemistry Theses & Dissertations

Purification of Yeast NAD⁺-dependent isocitrate dehydrogenase was partially successful when utilizing an NAD⁺-ribosyl-hydrazide Sepharose affinity chromatography column. Although no specific elution system was found, a maximum purification of sixty-five fold was achieved with 0.5M KCl elution. A 38-fold purification was obtained when 20mM NAD⁺ was employed for elution.

Yeast and calf liver NADP⁺-dependent isocitrate dehydrogenase were also found to bind to NAD⁺-ribosyl-hydrazide Sepharose as well as the NAD⁺-dependent enzyme. Again, elution was achieved with either o,5M KCl or 20 mM NADP⁺.

Ammonium sulfate fractionation decreased the yield …

Kinetic And Binding Studies On L-∝-Glycerophosphate Dehydrogenase, Paul Richard Rosevear

Chemistry & Biochemistry Theses & Dissertations

The properties of the coenzyme, NAD, binding site of chicken muscle L-∝-glycerophosphate dehydrogenase were studied. Adenosine monophosphate, adenosine diphosphate and adenosine diphosphoribose were sha.vn to inhibit the enzyme competitively with respect to NAD. The presence of adenosine, pyrophosphate and ribose regions in the coenzyme binding site of the enzyme was suggested by the inhibitor constants obtained for these compounds.

Disodium monoalkyl phosphates, n-butyl to n-dodecyl phosphate, inclusive, were also shown to inhibit the L-∝-glycerophosphate dehydrogenase catalized reaction competitively with respect to NAD. A positive chain length effect was observed in the binding of these compounds to the enzyme, suggesting the …

Sex Pheromone Activity In The Foveal Glands In The Two Species Of Ixodid Ticks: Dermacentor Andersoni (Stiles) And Dermacentor Variabilis (Say), Lynn Anne Collins

Biological Sciences Theses & Dissertations

Sex pheromone activity in the foveal glands of female Dermacentor andersoni (Stiles) and Dermacentor variabilis (Say) is described. Autoradiography revealed the accumulation of ³⁶Cl in the foveal glands of females during their first 120 hours of feeding on a ³⁶Cl-labeled host. Emissions collected from these females contained a ³⁶Cl-labeled product. This product, however, was absent in emissions collected from the same females with their foveae dorsales obstructed.

Accumulation of ³⁶Cl in the foveal gland, in conjunction with the release of a ³⁶Cl-labeled product through the foveae dorsales, suggest localization of sex pheromone activity within these …

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoretic Studies Demonstrating Cytoplasmic Protein Components Unique To The Mycelial Phase And To The Yeast Phase Of Candida Albicans, Theresa Catherine Burgess

Biological Sciences Theses & Dissertations

Blastospore and mycelial cytoplasmic extracts from four strains of Candida albicans, varying in virulence, were compared by sodium dodecyl sulfate (SDS) polyacrylamide disc gel electrophoresis Cytoplasmic extracts were made by disrupting the cultures in a Braun homogenizer followed by ultracentrifugation. The supernatant fractions were then resolved on polyacrylamide gels containing SDS. Each of the preparations yielded a distinct pattern. There were mycelial specific and blastospore specific protein bands. There were more components resolved from the blastospore phase cytoplasmic extracts than from the mycelial phase cytoplasmic extracts. There were bands common to both phases. These results demonstrate protein components unique …

Improved Techniques For Marking, Night-Viewing, And Aging White-Tailed Deer, Odocoileus Virginianus, Barnard Hodge Powell

Biological Sciences Theses & Dissertations

These studies were concerned with the development of methods to assist investigators of wildlife population research. Specifically they involve the description of (1) A device which enables an investigator to mark deer at medium range using specially modified darts fired by conventional tranquilizer rifles, (2) Construction of infrared viewers and light sources using surplus equipment and (3) Studies to develop a method of making permanent deer dental impressions under field conditions.

Similitude Of Mass Transfer Processes In Distorted Froude Model Of An Estuary, Carvel Hall Blair

Mechanical & Aerospace Engineering Theses & Dissertations

The project began with the design and construction of a hydraulic Froude model of the Lafayette River, a small well mixed estuary in Norfolk, Virginia. Horizontal scale is 1/540, vertical scale 1/12 yielding a vertical distortion of 45. Adjustment by roughness strips and screens produced close agreement of model-prototype tide heights, currents, and salinities in the deep reaches comprising 80% of the estuary volume. Some scale effect in velocity and tide height could not be eliminated in the shallow upper branches of the estuary, probably because of the high geometric distortion and the narrowness of the channel at kilometer 6.7. …

Control Of Clay Mineral Distribution By Estuarine Circulation, James River Estuary, Virginia, Jean-Pierre Feuillet

OES Theses and Dissertations

A study was performed on the clay minerals of the James River estuary, Virginia, to determine the predominant factors that influence clay mineral distribution in the estuary. The factors of differential settling, flocculation, and diagenesis show minor or no effects on distribution, while estuarine circulation exerts the strongest influence upon the clay mineral distribution.

There are two clay suites in the James River estuary. The upper James River clay suite is a kaolinite, dioctahedral vermiculite, and mixed-layer suite, with kaolinite being the major mineral. The lower Chesapeake Bay clay suite is an illite, chlorite, and montmorillonite suite, with illite being …

Variation In The Ecology Morphology And Behavior Of The Troglobitic Amphipod Crustacean Crangonyx Antennatus Packard (Crangonchidae) From Different Habitats, Gary Wayne Dickson

Biological Sciences Theses & Dissertations

Populations of the troglobitic amphipod Crangonyx antennatus living in two distinct aquatic habitats were examined for possible variations in their ecology, morphology and behavior. Collections were made seasonally for one year in six Lee Co., Virginia caves, three containing mud-bottom, drip pools and three with small gravel stream habitats. Environmental parameters thought to influence population variation were quantitatively and qualitatively recorded in each of the six caves.

Amphipod densities, body length, female maturity, clutch number and stream washout rates were found to be greater in drip pool habitat populations. Variation was also observed in body coloration and antennal segment-body length …

A Numerical Model For The Prediction Of Tides And Tidal Currents In The Lafayette River, Norfolk, Virginia, G. Mcallister Sisson

OES Theses and Dissertations

A prediction technique for tidal heights and currents in the Lafayette River of Norfolk, Virginia has been formulated and tested by the implementation of a two-dimensional numerical model employing the Navier-Stokes momentum equations and conservation of mass. The model operates on a spatially-fixed rectangular grid system by utilizing a multi-operation finite difference scheme which employs implicit and explicit solutions to the aforementioned equations. Forces due to bottom friction and Coriolis are taken into account. The only open boundary considered in the model is the river mouth, across which tidal height is specified temporally. Collected tidal height and current data at …

The Distribution Of Submersed Hydrophytes In Currituck Sound, Steven Harry Davis

Biological Sciences Theses & Dissertations

The rooted submergent flora of Currituck Sound, North Carolina was determined, and the distributions of its species investigated with respect to salinity, substrate texture, depth, pH, turbidity and species co-occurrence. Eight species of hydrophytes were found: Potamogeton pectinatus, Fotamogeton perfoliatus, Ruppia maritima, Zannichellia palustris, Najas guadalupensis, Elodea nuttallii, Vallisneria americana, and Myriophyllum spicatum. The mean salinities at which each species occurred ranged from 1.0 ppt for E. nuttallii to 3.1 ppt for Z. palustris, Ruppia maritima, Z palustris and V. americana were the only species that commonly occurred on coarse-grained substrates. Elodea nuttallii and N. guadalupensis …

A Batch Method For The Isolation Of Immunogenically Pure Igg, Iga, And Igm From Human Sera, Samuel Christopher Powell

Chemistry & Biochemistry Theses & Dissertations

A method is described for preparing an enriched fraction of the three major antibodies, immunoglobulins IgG, IgA and IgM, from normal human serum by a batch method. Selective precipitation of these proteins was carried out in successive steps using polyethylene glycol (PEG) of average molecular weight 6,000 at concentrations of 7%, 12% and 14% (16) . Zinc sulfate (10 mM) was used to remove highly immunogenic alpha₂ macroglobulin from the IgM fraction. IgA, IgM and IgG were further purified by adsorption methods using DEAE-cellulose. Quantitation and qualitation of the enriched fractions showed nearly complete separation of each of the …

Dynamics And Sedimentology Of Inner Middle Ground - Nine Foot Shoal, Chesapeake Bay, Virginia, Mitchell Alan Granat

OES Theses and Dissertations

The historical, hydrodynamic, and sedimentological development of Inner Middle Ground - Nine Foot Shoal indicates that it is presently in quasi-equilibrium with mutually evasive ebb and flood tidal currents. Bathymetric comparisons among six surveys taken during the past 122 years depict development of the shoal from two linear sand bodies to its present double parabolic morphology. Circulation paths are proposed for each survey.

Information from eight current meter stations on and adjacent to Inner Middle Ground - Nine Foot Shoal were used to determine near-surface and near-bottom tidal current asymmetries and directions. The concepts of bathymetrically diverted currents and mutual …

Test Variation In The Sand Dollar, Mellita Quinquiesperforata (Leske), George Glynn Rountree

OES Theses and Dissertations

Mellita quinguiesperforata is a common member of the benthos along the eastern coast of North America. This study considers variation of the test within the species, specifically: 1) variation in test weight, 2) variation in the length-width relationship, and 3) the average number of pore pairs in the ambulacral petals.

A total of 844 sand dollars from three localities was utilized: 1) Pass-a-Grill Beach, St. Petersburg, Florida,2) Bird Shoal, Beaufort, North Carolina, and 3) Fisherman Island, Virginia.

Animals were measured for length and width and the dry weight determined. Prediction equations for the size-weight relat1onships were computed for the three …

The Natural History Of The Naked Goby Gobiosoma Bosci (Perciformes: Gobiidae), Leonard L. Nero

OES Theses and Dissertations

The purpose of this study was to contribute to the knowledge of re ­ production, growth, age, abundance, habitat preference, feeding habit and behavior of the naked goby, Gobiosoma Bosci.

Monthly collections were made from January through December. 1974 in Deary Cove a apart of the Lynnhaven estuary, Virginia Beach, Virginia. All fish were measured and sexed in the field.

Most were returned to the population, the remainder. were removed for food habit analyses, determination of fecundity, degree of gonadal maturity and aging. Habitat­ preference and abundance were determined in the field.

Gobiosoma Bosci appears to be an annual …

Identify Competencies For Teachers Of The "World Of Transportation" In Industrial Arts, James Alexander Roth

OTS Master's Level Projects & Papers

It is the purpose of this study to determine and list the competencies needed by teachers of "World of Transportation" for evaluation by those concerned.

Investigating Methods Of Accelerating The Seasoning Of Wood Blanks For Bowl Turning, Myron W. Curtis

OTS Master's Level Projects & Papers

The purpose of the study was to establish through experimental research an improved method of accelerating the seasoning of bowl blanks. Specifically the study was conducted to determine: 1. Whether a worthwhile method or methods were available for accelerating the seasoning of wood bowl blanks; 2. Whether the blanks were of suitable quality; 3. Whether the method or methods present a worthwhile learning experience; 4. Whether the unseasoned wood and the equipment used in seasoning were available, safe, and within reasonable financial means.

Visual-Motor Skill Development In Learning Disabled Diagnosed Students Utilizing Industrial Arts Methods, Vernon M. Fueston Jr.

OTS Master's Level Projects & Papers

The purpose of this study was to determine the worth of an industrial arts unit of instruction in improving visual-motor development in Learning Disabled students.

A Curriculum Guide For Windmills, Bentley R. Harrell

OTS Master's Level Projects & Papers

No abstract provided.

A Comparison Of The Individualized And Traditional Approaches To Student Achievement In Photography At Churchland High School, James Earl Perkinson

OTS Master's Level Projects & Papers

The hypothesis for this research project is that there is no significant difference between the achievement of students taught photography by the traditional and "Individualized Learning Packets" approaches.