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Articles 61 - 90 of 8433
Full-Text Articles in Entire DC Network
Urinary 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine Analysis By An Improved Elisa: Does Assay Standardization Reduce Inter-Laboratory Variability?, Pavel Rossner Jr., Hilmi Orhan, Regina M. Santella, Kazuo Sakai, Gudrun Koppen, Antonin Ambroz, Andrea Rossnerova, Radim J. Sram, Miroslav Ciganek, Jiri Neca, Marcus Cooke
Urinary 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine Analysis By An Improved Elisa: Does Assay Standardization Reduce Inter-Laboratory Variability?, Pavel Rossner Jr., Hilmi Orhan, Regina M. Santella, Kazuo Sakai, Gudrun Koppen, Antonin Ambroz, Andrea Rossnerova, Radim J. Sram, Miroslav Ciganek, Jiri Neca, Marcus Cooke
Dr. Marcus S. Cooke PhD, FRCPath
ELISA is commonly used for the detection of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), a marker of whole body oxidative stress. However, the method has been criticized for high inter-laboratory variability and poor agreement with chromatographic techniques. We performed an inter-laboratory comparison of 8-oxodG assessed in 30 urine samples and a urine spiked with four different concentrations of 8-oxodG by ELISA using standardized experimental conditions, including: sample pre-treatment with solid-phase extraction (SPE), performing analysis using a commercial kit from a single manufacturer and strict temperature control during the assay. We further compared the ELISA results with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and …
Concurrent Beneficial (Vitamin D Production) And Hazardous (Cutaneous Dna Damage) Impact Of Repeated Low-Level Summer Sunlight Exposures, S. J. Felton, Marcus Cooke, R. Kift, J. L. Berry, A. R. Webb, P.M.W. Lam, F.R. De Gruijl, A. Vail, L. E. Rhodes
Concurrent Beneficial (Vitamin D Production) And Hazardous (Cutaneous Dna Damage) Impact Of Repeated Low-Level Summer Sunlight Exposures, S. J. Felton, Marcus Cooke, R. Kift, J. L. Berry, A. R. Webb, P.M.W. Lam, F.R. De Gruijl, A. Vail, L. E. Rhodes
Dr. Marcus S. Cooke PhD, FRCPath
No abstract provided.
Nucleotide Excision Repair Of Oxidised Genomic Dna Is Not A Source Of Urinary 8-Oxo-7,8-Dihydro-2’-Deoxyguanosine, Mark D. Evans, Vilas Misty, Rajinder Singh, Daniel Gackowski, Rafał Różalski, Agnieszka Siomek-Gorecka, David H. Phillips, Jie Zuo, Leon Mullenders, Alex Pines, Yusaku Nakabeppu, Kunihiko Sakumi, Mutsuo Sekiguchi, Teruhisa Tsuzuki, Margherita Bignami, Ryszard Oliński, Marcus Cooke
Nucleotide Excision Repair Of Oxidised Genomic Dna Is Not A Source Of Urinary 8-Oxo-7,8-Dihydro-2’-Deoxyguanosine, Mark D. Evans, Vilas Misty, Rajinder Singh, Daniel Gackowski, Rafał Różalski, Agnieszka Siomek-Gorecka, David H. Phillips, Jie Zuo, Leon Mullenders, Alex Pines, Yusaku Nakabeppu, Kunihiko Sakumi, Mutsuo Sekiguchi, Teruhisa Tsuzuki, Margherita Bignami, Ryszard Oliński, Marcus Cooke
Dr. Marcus S. Cooke PhD, FRCPath
Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is a widely measured biomarker of oxidative stress. It has been commonly assumed to be a product of DNA repair, and therefore reflective of DNA oxidation. However, the source of urinary 8-oxodGuo is not understood, although potential confounding contributions from cell turnover and diet have been ruled out. Clearly it is critical to understand the precise biological origins of this important biomarker, so that the target molecule that is oxidised can be identified, and the significance of its excretion can be interpreted fully. In the present study we aimed to assess the contributions of nucleotide excision repair …
European Contribution To The Study Of Ros: A Summary Of The Findings And Prospects For The Future From The Cost Action Bm1203 (Eu-Ros), Marcus Cooke
Dr. Marcus S. Cooke PhD, FRCPath
The European Cooperation in Science and Technology (COST) provides an ideal framework to establish multi-disciplinary research networks. COST Action BM1203 (EU-ROS) represents a consortium of researchers from different disciplines who are dedicated to providing new insights and tools for better understanding redox biology and medicine and, in the long run, to finding new therapeutic strategies to target dysregulated redox processes in various diseases. This report highlights the major achievements of EU-ROS as well as research updates and new perspectives arising from its members. The EU-ROS consortium comprised more than 140 active members who worked together for four years on the …
8-Oxo-7,8-Dihydroguanine And 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine Concentrations In Various Human Body Fluids: Implications For Their Measurement And Interpretation, Chiung-Wen Hu, Marcus Cooke, Yi-Hung Tsai, Mu-Rong Chao
8-Oxo-7,8-Dihydroguanine And 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine Concentrations In Various Human Body Fluids: Implications For Their Measurement And Interpretation, Chiung-Wen Hu, Marcus Cooke, Yi-Hung Tsai, Mu-Rong Chao
Dr. Marcus S. Cooke PhD, FRCPath
8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is the most investigated product of oxidatively damaged DNA lesion that has been associated with the development of aging, cancer and some degenerative diseases. Here, we present the first liquid chromatography-tandem mass spectrometry method that enables the simultaneous measurement of its repair products in plasma and saliva, namely 8-oxo-7,8-dihydroguanine (8-oxoGua) and 8-oxodGuo. Using this method, we investigated the underlying transport mechanism of the repair products of oxidatively damaged DNA between cellular compartments and biological matrices. Plasma, saliva and urine samples were collected concurrently from 57 healthy subjects. Various deproteinization methods were evaluated, and the precipitants acetonitrile and sodium …
Rescue Of Cells From Apoptosis Increases Dna Repair In Uvb Exposed Cells: Implications For The Dna Damage Response, Mahsa Karbaschi, Salvador Macip, Vilas Mistry, Hussein H.K. Abbas, George J. Delinassios, Mark D. Evans, Anthony R. Young, Marcus Cooke
Rescue Of Cells From Apoptosis Increases Dna Repair In Uvb Exposed Cells: Implications For The Dna Damage Response, Mahsa Karbaschi, Salvador Macip, Vilas Mistry, Hussein H.K. Abbas, George J. Delinassios, Mark D. Evans, Anthony R. Young, Marcus Cooke
Dr. Marcus S. Cooke PhD, FRCPath
Classically, the nucleotide excision repair (NER) of cyclobutane pyrimidine dimers (CPD) is a lengthy process (t1/2 > 48 h). Using the T4 endonuclease V-modified comet assay, we uniquely found a far more rapid repair of UVA-induced CPD (t1/2 = 4.5 h) in human skin keratinocytes. The repair of UVB-induced CPD began to slow within 1 h of irradiation, causing damage to persist for over 36 h. A similar trend was noted for the repair of oxidatively-modified purine nucleobases. Supportive of this differential repair, we noted an up-regulation of key genes associated with NER in UVA-irradiated cells, whereas the same …
Novel Method For The High-Throughput Processing Of Slides For The Comet Assay, Mahsa Karbaschi, Marcus Cooke
Novel Method For The High-Throughput Processing Of Slides For The Comet Assay, Mahsa Karbaschi, Marcus Cooke
Dr. Marcus S. Cooke PhD, FRCPath
Single cell gel electrophoresis (the comet assay), continues to gain popularity as a means of assessing DNA damage. However, the assay’s low sample throughput and laborious sample workup procedure are limiting factors to its application. “Scoring”, or individually determining DNA damage levels in 50 cells per treatment, is time-consuming, but with the advent of high-throughput scoring, the limitation is now the ability to process significant numbers of comet slides. We have developed a novel method by which multiple slides may be manipulated, and undergo electrophoresis, in batches of 25 rather than individually and, importantly, retains the use of standard microscope …
作为大理论、元理论、预设理论与预测理论 的选择螺旋理论 (Selective Spiral – A Mega, Meta, Predictive And Presumptive Theory), Xinshu Zhao
作为大理论、元理论、预设理论与预测理论 的选择螺旋理论 (Selective Spiral – A Mega, Meta, Predictive And Presumptive Theory), Xinshu Zhao
Professor Xinshu ZHAO
No abstract provided.
Survey Instrument: Data Challenges Of Biomedical Researchers In The Age Of Omics, Rolando Garcia-Milian, Denise Hersey, Milica Vukmirovic
Survey Instrument: Data Challenges Of Biomedical Researchers In The Age Of Omics, Rolando Garcia-Milian, Denise Hersey, Milica Vukmirovic
Rolando Garcia-Milian
No abstract provided.
Interview Questionaire: Data Challenges Of Biomedical Researchers In The Age Of Omics, Rolando Garcia-Milian, Denise Hersey, Milica Vukmirovic
Interview Questionaire: Data Challenges Of Biomedical Researchers In The Age Of Omics, Rolando Garcia-Milian, Denise Hersey, Milica Vukmirovic
Rolando Garcia-Milian
No abstract provided.
Teaching Statistics To Msw Students: Comparing Credit And Non-Credit Options, Ashley Davis, Rebecca G. Mirick
Teaching Statistics To Msw Students: Comparing Credit And Non-Credit Options, Ashley Davis, Rebecca G. Mirick
Rebecca Mirick
Understanding Sampling And Recruitment In Social Work Dissertation Research, Rebecca G. Mirick, Ashley Davis, Stephanie P. Wladkowski
Understanding Sampling And Recruitment In Social Work Dissertation Research, Rebecca G. Mirick, Ashley Davis, Stephanie P. Wladkowski
Rebecca Mirick
School Counselors Walking The Walk And Talking The Talk: A Grounded Theory Of Effective Program Implementation, Janna L. Scarborough, Melissa Luke
School Counselors Walking The Walk And Talking The Talk: A Grounded Theory Of Effective Program Implementation, Janna L. Scarborough, Melissa Luke
Janna L. Scarborough
Comprehensive, developmental school counseling programming has been associated with numerous benefits for students and is considered current best practice. A qualitative, grounded theory study was conducted to investigate eight professional school counselors employed across grade level, geographic setting, and region within the United States. This article presents this research and the emergent model for successful comprehensive, developmental school counseling program implementation. Implications for school counselor education and practice, as well as future research, are discussed.
Connections Through Clubs: Collaboration And Coordination Of A Schoolwide Program, Wendy Logan, Janna L. Scarborough
Connections Through Clubs: Collaboration And Coordination Of A Schoolwide Program, Wendy Logan, Janna L. Scarborough
Janna L. Scarborough
The "Connections Through Clubs" program provides all students with the opportunity to participate in a small-group extracurricular activity and mentoring experience led by school faculty, staff, and community members during the school day throughout the academic year. This schoolwide program was developed in response to identified school needs and as a means to facilitate a strengths-enhancing school environment and to promote the developmental competencies of all students, both of which have been linked to academic and personal success. The purpose of this article is to describe the inception, implementation, and preliminary evaluation of the Connections Through Clubs program. In addition, …
Marina365 Agen Casino Online, Judi Casino, Casino Online Terbaik.Pdf, Sulis Setyo Sulis Setyo
Marina365 Agen Casino Online, Judi Casino, Casino Online Terbaik.Pdf, Sulis Setyo Sulis Setyo
Sulis Setyo
Are We At A Turning Point For Distance Research In International Business Studies?, Douglas Dow
Are We At A Turning Point For Distance Research In International Business Studies?, Douglas Dow
Douglas Dow
Burnout Syndrome In Hospital's Nurses: Causes And Consequences, Lama Bakhamis, Harlan M. Smith Ii, Alberto Coustasse, David P. Paul Iii
Burnout Syndrome In Hospital's Nurses: Causes And Consequences, Lama Bakhamis, Harlan M. Smith Ii, Alberto Coustasse, David P. Paul Iii
Harlan M. Smith
PURPOSE: The purpose of this research was to examine the causes and consequences of Burnout Syndrome among RNs in U.S. hospitals to identify solutions to this problem.
METHODS: The methodology was the review of the literature and a semi-structured interview. There were seven primary databases and two websites used in this research, and 35 articles were used for this literature review.
RESULTS: Causes and risk factors of burnout syndrome among RNs have been categorized into four major areas: individual, management, organizational, and work characteristics. Burnout syndrome rate among RNs with age under 30 years was 43.6% higher …
Burnout Syndrome In Hospital's Nurses: Causes And Consequences, Lama Bakhamis, Harlan M. Smith Ii, Alberto Coustasse, David P. Paul Iii
Burnout Syndrome In Hospital's Nurses: Causes And Consequences, Lama Bakhamis, Harlan M. Smith Ii, Alberto Coustasse, David P. Paul Iii
Alberto Coustasse, DrPH, MD, MBA, MPH
PURPOSE: The purpose of this research was to examine the causes and consequences of Burnout Syndrome among RNs in U.S. hospitals to identify solutions to this problem. METHODS: The methodology was the review of the literature and a semi-structured interview. There were seven primary databases and two websites used in this research, and 35 articles were used for this literature review. RESULTS: Causes and risk factors of burnout syndrome among RNs have been categorized into four major areas: individual, management, organizational, and work characteristics. Burnout syndrome rate among RNs with age under 30 years was 43.6% higher than RNs over …
The Research Needs And Practices Of Asian Studies Scholars At Trinity University: A Report For Ithaka S+R, Michael Hughes
The Research Needs And Practices Of Asian Studies Scholars At Trinity University: A Report For Ithaka S+R, Michael Hughes
Michael J. Hughes
This report describes the research needs and practices of nine Asian Studies scholars at Trinity University, a private liberal arts college in San Antonio, Texas. Part of a nationwide study coordinated by Ithaka S+R, the report describes scholars’ 1 methods, information needs, and publication practices in order to better align and deliver research support from academic departments, librarians, university administrators, and other stakeholders. The report culminates in predictions on the future of the field, and offers several recommendations to help scholars achieve the future they envision for Asian Studies.
Personal Values Of Principals And Their Manifestation In Student Behaviour : A District-Level Study In Pakistan, Christopher Branson, Sharifullah Baig, Abida Begum
Personal Values Of Principals And Their Manifestation In Student Behaviour : A District-Level Study In Pakistan, Christopher Branson, Sharifullah Baig, Abida Begum
Sharifullah Baig
Although there is growing research evidence to support the view that the leadership practice of the school principal is the second-most important influence on student learning behind classroom teaching, there is no clarity about what, exactly, the principal is meant to do to ensure this outcome. Hence, Leithwood et al. (2010) propose that one of the principal’s important influences on student learning is the ‘rational’ path, which includes the issue of school-wide disciplinary climate. This argues that the principal plays a pivotal role in establishing the school-wide disciplinary climate that aids student learning. This article reports upon research conducted in …
Academic Freedom For Teachers, Larry L. Kraus, Robert L. Stevens
Academic Freedom For Teachers, Larry L. Kraus, Robert L. Stevens
Robert Stevens
The concept of academic freedom is a basic right, if not a Constitutional right, of educators in the classroom and in research. While freedom in research and teaching has traditionally been assumed to be more in the realm of higher education, the ability to make reasonable decisions related to presentation of academic content has been accepted at the K-12 levels as a basic responsibility of instruction.
Fourth And Fifth Amendment Decisions: Students And Constitutional Rights, Larry L. Kraus, Robert L. Stevens
Fourth And Fifth Amendment Decisions: Students And Constitutional Rights, Larry L. Kraus, Robert L. Stevens
Robert Stevens
The article reviews constitutional rights for students, focusing on the 4th and 5th amendment.
Limitations Of Communication, Alfonso Ilipollito, Robert L. Stevens
Limitations Of Communication, Alfonso Ilipollito, Robert L. Stevens
Robert Stevens
As college teachers we have a responsibility to communicate to our students, not only for content which leads to understanding the subject matter we teach, but also to motivate and engage students to want to learn that content. Our student body is changing rapidly. Many of us teach not only “first generation” college students, but students that reflect our country’s diversity as well. To be more effective communicators we need to better understand the communication dynamics this change imposes on us.
"Introduction." In Don Quixote: The Re-Accentuation Of The World’S Greatest Literary Hero, Slav N. Gratchev Phd
"Introduction." In Don Quixote: The Re-Accentuation Of The World’S Greatest Literary Hero, Slav N. Gratchev Phd
Dr. Slav N. Gratchev
This book is a unique scholarly attempt to examine Don Quixote from multiple angles to see how the re-accentuation of the world’s greatest literary hero takes place in film, theatre, and literature. To accomplish this task, nineteen scholars from the United States, Canada, Spain, and Great Britain have come together, and each of them has brought his/her unique perspective to the subject. For the first time, Don Quixote is discussed from the point of re-accentuation, that is, having in mind one of the key Bakhtinian concepts that will serve as a theoretical framework. A primary objective was therefore to articulate, …
Adventures With Animals Big And Small, Emily Allen, Marcus Blandford, Shannon Brennan, Brennen Keen, Amanda Timm, Tara Penry, Sarah Obendorf
Adventures With Animals Big And Small, Emily Allen, Marcus Blandford, Shannon Brennan, Brennen Keen, Amanda Timm, Tara Penry, Sarah Obendorf
Tara Penry
The purpose of this project is to produce a short collection of out-of-print children’s stories that would be suitable for first grade level readers. Stories selected for the collection fit the theme of being seasonally themed and include animals as main protagonists. Under the guidance of Dr. Tara Penry, the class searched children’s magazines from the late 1800’s and early 1900’s to find stories that would be relevant and interesting to today’s elementary schoolers.
Progressive Foote? Gender Politics In An 1887 Letter From Mary Hallock Foote, Tara Penry
Progressive Foote? Gender Politics In An 1887 Letter From Mary Hallock Foote, Tara Penry
Tara Penry
Mary Hallock Foote is not known for progressive gender politics. Quite the opposite. As her biographer Darlis Miller observes, Foote and her longtime friend Helena DeKay Gilder agreed that woman’s most important work lay in the home, and suffrage would distract her from her primary duties. But Foote did not always practice her belief in the separate spheres of men and women perfectly. Not only did necessity compel her for a time to support her family, but an 1887 letter also shows that in her professional life, Foote did not always think of her work as feminine or separate from …
Why France Needs To Collect Data On Racial Identity... In A French Way, David B. Oppenheimer
Why France Needs To Collect Data On Racial Identity... In A French Way, David B. Oppenheimer
David B Oppenheimer
No abstract provided.
Negligent Discrimination, David Benjamin Oppenheimer
Negligent Discrimination, David Benjamin Oppenheimer
David B Oppenheimer
No abstract provided.
Kennedy, King, Shuttlesworth And Walker: The Events Leading To The Introduction Of The Civil Rights Act Of 1964, David Benjamin Oppenheimer
Kennedy, King, Shuttlesworth And Walker: The Events Leading To The Introduction Of The Civil Rights Act Of 1964, David Benjamin Oppenheimer
David B Oppenheimer
No abstract provided.
Mcdonnell Douglas Corp. V. Green Revisited: Why Non-Violent Civil Disobedience Should Be Protected From Retaliation By Title Vii, David Benjamin Oppenheimer
Mcdonnell Douglas Corp. V. Green Revisited: Why Non-Violent Civil Disobedience Should Be Protected From Retaliation By Title Vii, David Benjamin Oppenheimer
David B Oppenheimer
No abstract provided.