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Articles 31 - 60 of 192
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Interactions Of Arbuscular Mychorrhizal Fungi And Bacterial Endophytes On Disease Resistance Of Common Root Pathogens In Wheat, Janice L. Eibensteiner
Interactions Of Arbuscular Mychorrhizal Fungi And Bacterial Endophytes On Disease Resistance Of Common Root Pathogens In Wheat, Janice L. Eibensteiner
Electronic Theses and Dissertations
Arbuscular mycorrhiza, a symbiosis between plants and fungi, help plants to capture nutrients such as phosphorus (P), nitrogen (N) and other micronutrients from the aggre in exchange for up to 20% of the fixed carbon (C) from the plant. In addition, arbuscular mycorrhizal (AM) fungi can improve the resistance against abiotic (drought, salinity), and also biotic (pathogen) stresses. Bacterial endophytes promote plant growth and yield by fixing N2 from the atmosphere, assimilating N and transfer it to the plant. Bacterial endophytes can also solubilize phosphate and stimulate plant defense responses, suppressing pathogens. The host plant provides sucrose and a favorable …
The Relationship Between Campus Wellness Center Usage And Symptoms Of Depression In College Freshmen, Allison Leonard
The Relationship Between Campus Wellness Center Usage And Symptoms Of Depression In College Freshmen, Allison Leonard
Electronic Theses and Dissertations
There is limited research done on the relationship between the program utilization at a campus wellness facility and the symptoms of depression in college freshmen. College students have been found to have a higher prevalence of depressive symptoms than the general population, possibly due to the stressors college life can add. Studies have been done on the effects of physical activity as an intervention for depression as well as on the benefits of campus wellness facilities; however, there have been few studies that look at both campus recreation and depression. The author’s purpose for this study was to see if …
Development Of A Novel Nanotechnology-Based Vaccine Adjuvant And Delivery System For Systemic And Mucosal Immunity, Mohammed Ali Bakkari
Development Of A Novel Nanotechnology-Based Vaccine Adjuvant And Delivery System For Systemic And Mucosal Immunity, Mohammed Ali Bakkari
Electronic Theses and Dissertations
Many serious infections for which there are no existing vaccines, enter by a mucosal route such as HIV, influenza, and tuberculosis, etc. Therefore, designing safe and effective mucosal vaccines that elicit robust immune response at mucosal sites is still a major challenge without a safe mucosal adjuvant. Previously, our laboratory had discovered inulin acetate as a novel toll-like-receptor-4 agonist (adjuvant). The study in this dissertation explored the application of nanoparticles prepared with inulin acetate (InAc-NPs) as a vaccine adjuvant and delivery system for enhancing mucosal immunity. The rationale behind selecting InAc-NPs is their proven ability to activate strong systemic immunity …
Evaluation Of The Effect Of Carvedilol On The Thioredoxin Pathway In H9c2 Rat Cardiomyocytes, Metab Alharbi
Evaluation Of The Effect Of Carvedilol On The Thioredoxin Pathway In H9c2 Rat Cardiomyocytes, Metab Alharbi
Electronic Theses and Dissertations
The thioredoxin (Trx) system is an endogenous antioxidant system that affects cell function and survival through controlling cellular redox status. Trx and TrxR are the main enzymes in this system while thioredoxin interacting protein (TXNIP) is a negative regulator. This study’s goal was to better understand the Trx system’s involvement in the cardiovascular disease and modulate the pathway through drug treatment. Carvedilol is a non-selective β-blocker that also exhibits antioxidant properties, but the exact mechanism of the antioxidant effect is still unclear. H9c2 rat cardiomyocytes were used to examine the effect of carvedilol on the Trx system under normal conditions. …
Natural Sunlight Photodegradation Of Halogenated Disinfection Byproducts In Water, Ibrahim Abusallout
Natural Sunlight Photodegradation Of Halogenated Disinfection Byproducts In Water, Ibrahim Abusallout
Electronic Theses and Dissertations
Disinfection byproducts (DBPs) presence in wastewater effluents and receiving waters may impact the quality of drinking water during water reuse practices. Natural solar photolysis is one of the biogeochemical processes that may lead to decreased DBPs concentrations in water. The purpose of this dissertation is to determine the fate of chlorinated, brominated and iodinated DBPs in surface water by natural sunlight photolysis and investigate the use of solar-based advanced oxidation processes (AOPs) for removal of DBPs in water. Total organic halogen (TOX) was used to measure total chlorinated- (TOCl), brominated- (TOBr) and iodinated-DBPs (TOI) in water. The first objective was …
Effect Of Mineral Ions On The Functional Properties Of Starch Films, Aliza Sigdel
Effect Of Mineral Ions On The Functional Properties Of Starch Films, Aliza Sigdel
Electronic Theses and Dissertations
Essential minerals are indispensable inorganic micronutrients that modulate vital physiological functions at the molecular level. Their deficiency, though required in small amounts, impairs health significantly. Their supplementation through diet is prudent and carbohydrates standout as favorable choice. Carbohydrates, starches and polysaccharides, exhibit unique chemical structures and functionalities, and interact with mineral ions in several ways. The aim of this research is to investigate the effect of mineral ions on the physicochemical properties, tensile strength and in vitro starch digestion of starch-films. Corn starch and potato starch along with Fe2+, Mn2+, Cu2+ and Zn2+ ions have been chosen as model starch …
Repairable Moment-Resisting Steel Buildings, Heath William Pederson
Repairable Moment-Resisting Steel Buildings, Heath William Pederson
Electronic Theses and Dissertations
Current seismic design codes ensure life safety for buildings, but structural members may significantly yield or even fail under strong earthquakes. A new design approach is to implement connections that localize the yielding and failure to fuses. A more sophisticated approach is to be able to replace the fuses after the event. The present study was carried out to develop repairable moment-resisting (MR) connections for steel buildings and to investigate their seismic performance through nonlinear finite element analyses (FEA). Two details were proposed using buckling restrained fuses (BRFs) and buckling restrained reinforcement (BRR). Test data was collected from the literature …
A Review Of The Genus Acrisis Foerster (Hymenoptera: Braconidae) With A New Species From American Licorice And A Survey Of Aphid Parasitoids Of Soybean Fields In Eastern South Dakota, Abigail P. Martens
Electronic Theses and Dissertations
The diversity of parasitoid wasps (Hymenoptera: Braconidae) in South Dakota is largely unexamined despite these wasps providing innumerable important ecological and biological services and being an essential part of natural and agricultural ecosystems. A new species of Acrisis Foerster was reared and is described from the native prairie legume Glycyrrhiza lepidota (Nuttall) Pursh, a plant studied for its potential for conservation and remediation. A large insect guild of seed predators and their associated parasitoids and hyperparasitoids reported from G. lepidota provides a list of potential hosts associated with the new parasitoid species. The agriculturally significant legume soybean, Glycine max (L.) …
Identifying An Optimal Bald Eagle Monitoring Program For Southwest Alaska National Parks, Rebecca Kolstrom
Identifying An Optimal Bald Eagle Monitoring Program For Southwest Alaska National Parks, Rebecca Kolstrom
Electronic Theses and Dissertations
The Southwest Alaska Inventory and Monitoring Network includes bald eagle monitoring as part of their Vital Signs Monitoring Plan. Lake Clark National Park and Preserve, Katmai National Park and Preserve, Kenai Fjords National Park, and Wrangell – St. Elias National Park and Preserve monitor bald eagles annually, albeit slightly differently among parks. Since monitoring decisions involve multiple objectives and stakeholders, there was a need for a structured approach to identify an optimal monitoring program. We used a structured decision making process and an iterative, four-round Delphi Process to collect information about long-term bald eagle monitoring from experts. We collected information …
Estimating Soil Organic Carbon In Cultivated Soils Using Soil Test Data, Remote Sensing Imagery From Satellites (Landsat 8 And Plantscope), And Web Soil Survey Data, Muhammed Halil Koparan
Estimating Soil Organic Carbon In Cultivated Soils Using Soil Test Data, Remote Sensing Imagery From Satellites (Landsat 8 And Plantscope), And Web Soil Survey Data, Muhammed Halil Koparan
Electronic Theses and Dissertations
Soil organic carbon (SOC) is an important soil parameter of cultivated soils that needs to be monitored and mapped regularly to enhance soil health and productivity. SOC levels in cultivated areas is difficult to monitor for farmers and is costly to analyze using traditional methods. The objective of this study was to estimate surface SOC distribution in selected soils of Major Land Resource Areas (MLRA) 102A (Rolling Till Plain, Brookings County, SD) and 103 (Central Iowa and Minnesota Till Prairies, Lac qui Parle County, MN) using soil sample data, Web Soil Survey (WSS) data, and satellite imagery (Landsat 8 and …
Identification And Characterization Of Stress Responsive Genes In Soybean And Sunflower, Surendra Neupane
Identification And Characterization Of Stress Responsive Genes In Soybean And Sunflower, Surendra Neupane
Electronic Theses and Dissertations
Stress responsive genes encode proteins involved in plants’ response to abiotic and biotic stresses. Among such stress responsive proteins, proteins encoded by resistance genes (R genes) or nucleotide binding site-leucine-rich repeats (NBS-LRRs) and mitogen-activated protein kinases (MAPKs) are the major groups of proteins regulating biotic and abiotic stresses, respectively. Previous studies in Nepal’s lab at SDSU identified and characterized coiled coil (CC)-NBS-LRRs (CNLs), resistance to powdery mildew8 (RPW8)-NBS-LRRs (RNLs), NBS-LRR (NLs), and MAPK proteins in soybean. This study focuses on R and MAPK genes in the recently sequenced genome of sunflower as well as the toll-interleukin-1 receptor-like nucleotide-binding site leucine-rich …
Development And Evaluation Of Vaccine Candidates For Senecavirus A, Bishwas Sharma
Development And Evaluation Of Vaccine Candidates For Senecavirus A, Bishwas Sharma
Electronic Theses and Dissertations
Senecavirus A (SVA) has been associated with several outbreaks of vesicular disease (VD) since 2014 in major swine-producing countries around the world. The virus causes VD that is clinically indistinguishable from foot-and-mouth disease (FMD) in pigs. Currently there are no commercial vaccines available for SVA. Here, we developed a live attenuated SVA strain by mutating a wild type SVA strain SD15-26 (wt SVA). The live attenuated virus, rSVA mSacII, replicated less efficiently in cell cultures in vitro and expressed lower amounts of VP1 and VP2 capsid proteins than wt SVA. Most importantly, the rSVA mSacII virus failed to induce clinical …
Investigation Of Intergroup Bias In Two Neuromaturationally Distinct Age Cohorts: An Erp Study, Reuven M. Hanna
Investigation Of Intergroup Bias In Two Neuromaturationally Distinct Age Cohorts: An Erp Study, Reuven M. Hanna
Electronic Theses and Dissertations
Currently, sociological investigation of adolescent behavior focuses on the intersection of biography, history, and structure to explain adolescent risk-taking, reward-seeking, impulsivity, novelty-seeking and peer-salience. However, the preponderance of the evidence points away from social ecology and to a significant neuromaturational restructuring event between the 12th and 25th years of life as the root of adolescent behavioral tendencies. As a result, sociological social psychology can benefit from engaging in basic research using neuroscience methods. The present study expands the dual systems model of brain development to account for maturational changes in the social brain network as a way to explain social …
Development And Characterization Of Wheat-Thinopyrum Junceiforme Chromosome Addition Lines, Dilkaran Singh
Development And Characterization Of Wheat-Thinopyrum Junceiforme Chromosome Addition Lines, Dilkaran Singh
Electronic Theses and Dissertations
Production of wheat is challenged by dynamic biotic and abiotic stresses. Genetic improvement via alien gene transfer is an effective approach to tackle such challenges. Alien gene transfer played an important role in the history of wheat crop improvement. Sea wheatgrass (SWG; Thinopyrum junceiforme, 2n = 28, genomes J1J1J2J2) is a wild relative of wheat. In our previous work, we have developed a complete amphiploid between cultivated emmer and SWG and shown that SWG is resistant to wheat streak mosaic virus, Fusarium head blight and wheat stem sawflies (due to the solid stem) and tolerant to waterlogging, salinity, heat, and …
Assessing Freshwater Mussel (Bivalvia: Unionidae) Assemblages And Effects Of Eutrophication On Pyganodon Grandis In Lakes Of Eastern South Dakota, Katherine M. Wollman
Assessing Freshwater Mussel (Bivalvia: Unionidae) Assemblages And Effects Of Eutrophication On Pyganodon Grandis In Lakes Of Eastern South Dakota, Katherine M. Wollman
Electronic Theses and Dissertations
Globally, the family Unionidae is the most threatened group of freshwater fauna. South Dakota is well known for its agricultural production, leading to cultural eutrophication from enhanced fertilizers and sediments. This impact can alter species presence, age, growth and mortality. Objectives of this study were to 1) implement the first comprehensive lake survey of freshwater mussels in eastern South Dakota lakes and document distribution, occurrence and relative abundance, 2) evaluate relationships between age, growth and mortality of Pyganodon grandis and lake trophic state. Freshwater mussels were sampled in 2017 from 116 proportionally and randomly selected natural lakes and reservoir basins …
Food Assistance Programs In The Us And Their Impact On Childhood Obesity, Jannatul Kawsar
Food Assistance Programs In The Us And Their Impact On Childhood Obesity, Jannatul Kawsar
Electronic Theses and Dissertations
Numerous food assistance programs (FAP) aim to ensure food security among low income American households. However, the literature suggests that participation in food assistance programs may be associated with the US childhood obesity epidemic. The goal of this study is to analyze the association between the number of major food assistance programs children participate in and childhood obesity. The major food assistance programs considered are the Supplemental Nutrition Assistance Program (SNAP), the Special Supplemental Food Program for Women, Infants, and Children (WIC), the National School Lunch Program (NSLP), and the School Breakfast Programs (SBP). This is the first study to …
Efficacy Of Synthetic And Biopesticides On Bacteria Leaf Streak Management And Influence Of Cultivar And Environment On Epiphytic Bacteria Diversity On Wheat Seeds, Marilen Nampijja
Electronic Theses and Dissertations
Wheat (Triticum aestivum) is the most important staple food for over two billion people, or 36% of the world population. The United States ranks 4th in the world for wheat production. However, wheat production is faced by both biological and non-biological constraints. Among the biological constraints, diseases play a big role in limiting wheat production with estimated yield losses ranging from 20% to 40%. Bacterial leaf streak (BLS) of wheat caused by Xanthomonas translucens pv undulosa (Xtpvu) is one of the major bacterial diseases affecting the wheat production in most wheat growing regions in the USA. In recent years, it …
A Study Of The Effect Of Heat Treatment On 3d Printed Pla Impact Strength, Suresh Thota
A Study Of The Effect Of Heat Treatment On 3d Printed Pla Impact Strength, Suresh Thota
Electronic Theses and Dissertations
Environmental conditions have a significant effect on the mechanical properties of various materials. Environmental parameters such as temperature and humidity have a major impact on mechanical properties of materials such as compressive strength, tensile strength, bending strength and impact strength. The purpose of this research is to study how temperature and humidity affect the impact strength of 3D printed PLA plastic. Impact strength is the ability of a material to absorb energy subjected to an impact load by a pendulum. In this research, 3D printing was employed to produce PLA specimens which were later used for different experimental testings. For …
Development Of Computational Techniques For Identification Of Regulatory Dna Motif, Cankun Wang
Development Of Computational Techniques For Identification Of Regulatory Dna Motif, Cankun Wang
Electronic Theses and Dissertations
Identifying precise transcription factor binding sites (TFBS) or regulatory DNA motif (motif) plays a fundamental role in researching transcriptional regulatory mechanism in cells and helping construct regulatory networks for biological investigation. Chromatin immunoprecipitation combined with sequencing (ChIP-seq) and lambda exonuclease digestion followed by high-throughput sequencing (ChIP-exo) enables researchers to identify TFBS on a genome-scale with improved resolution. Several algorithms have been developed to perform motif identification, employing widely different methods and often giving divergent results. In addition, these existing methods still suffer from prediction accuracy. Thesis focuses on the development of improved regulatory DNA motif identification techniques. We designed an …
Effects Of Increasing Dietary Levels Of Carinata Meal On Feeding Behavior, Performance And Carcass Characteristics Of Beef Growing Steers, Alejandro C. Casella
Effects Of Increasing Dietary Levels Of Carinata Meal On Feeding Behavior, Performance And Carcass Characteristics Of Beef Growing Steers, Alejandro C. Casella
Electronic Theses and Dissertations
The objectives of the present study are to evaluate feeding behavior, animal performance and carcass characteristics of beef growing steers fed increasing amounts of carinata meal (CM). Due to the high protein content of the CM after the oil extraction for biofuel there is an opportunity to use it as a protein supplementation in beef cattle. Twenty-four steers blocked by weight were assigned to four corn-based diets (n = 6 animals per treatment). In the three treatment diets, carinata meal replaced high-moisture corn at 5, 10, or 15%. The diets were fed using the Insentec RIC system for 138 days. …
Production And Storage Stability Of High Concentrated Micellar Casein And Its Effect On The Functional Properties Of Process Cheese Products, Ahmed Hammam
Electronic Theses and Dissertations
Micellar casein is a high-protein ingredient that can be used in process cheese products (PCP) formulations. PCP is a dairy food prepared by blending dairy ingredients (such as natural cheese, protein concentrates, butter, non-fat dry milk NFDM, whey powder, and permeate) with nondairy ingredients (such as sodium chloride, water, emulsifying salts, color, and flavors) and then heating the mixture with a continuous agitation to produce a homogeneous product with an extended shelf-life. The first objective of this study was to produce a highly concentrated micellar casein (HC-MC) and evaluate its storage stability. Skim milk was pasteurized at 76°C for 16 …
Spirituality, Religiosity, And Perceived Ability To Provide Spiritual Care In Nursing Students, Shannon O'Connell-Persaud
Spirituality, Religiosity, And Perceived Ability To Provide Spiritual Care In Nursing Students, Shannon O'Connell-Persaud
Electronic Theses and Dissertations
Objective. The objective of this study was to explore nursing students’ spirituality, religiosity, and perceived ability to provide spiritual care. Background. Nursing students must be prepared to respond to a patient’s spiritual needs. A gap in knowledge exists as to the educational preparedness necessary to provide a nursing student with the ability to respond to a patient’s spiritual need. To address the gap, this study explored the relationships between nursing students’ spirituality, religiosity, and perceived ability to provide spiritual care. Study Setting and Sample. This multi-site study used two accredited nursing programs located in the Western Region of the United …
Application Of Analytical Techniques In Food Product Development, Sonali R. Pandey
Application Of Analytical Techniques In Food Product Development, Sonali R. Pandey
Electronic Theses and Dissertations
Products are analyzed in major sectors of food industry to meet regulatory standards, food safety, quality control, and research and development. Analytical and extraction techniques have been applied for simultaneous investigation of multiple product characteristics. Food analysis faces critical challenges due to the complex composition of food, leading to matrix effects that result in loss of accuracy and reliability. The analytical techniques require an efficient sample preparation to avoid or minimize matrix effects. Solid-phase extraction has been widely used to address this issue. In this dissertation, the aim is to apply analytical techniques in food product development. To accomplish this, …
Winter Snow Depth In Arctic Alaska Results In Complex Changes In Caribou Forage Quality, Jessica C. Richert
Winter Snow Depth In Arctic Alaska Results In Complex Changes In Caribou Forage Quality, Jessica C. Richert
Electronic Theses and Dissertations
Caribou (Rangifer tarandus) rely on the short growing season for much of their annual nutrition, making them susceptible to even small changes in forage quantity and quality. Body condition in the summer and fall is linked to winter survival rates and fecundity in cows, critical factors in the robustness of caribou populations. Due to a warmer, wetter climate, snowfall is predicted to increase over Alaska’s North Slope in the next several decades. Deeper snow results in higher soil temperatures, allowing microbial mineralization of nitrogen to continue throughout the winter and increasing the availability of nitrogen for plants in spring and …
Optimization Of Reproductive Success In Beef Cattle By Implementation Of Estrus Resynchronization, Nutritional Management, And Evaluation Of Disease Prevalance, Kaitlin M. Epperson
Optimization Of Reproductive Success In Beef Cattle By Implementation Of Estrus Resynchronization, Nutritional Management, And Evaluation Of Disease Prevalance, Kaitlin M. Epperson
Electronic Theses and Dissertations
The beef industry is a unique community of operations that employ different management techniques to accomplish a similar goal. Like other facets of animal agriculture, profitability of the beef industry is heavily reliant upon the implemented management practices. Reproductive efficiency is an area of management that has great potential to positively influence the success of beef production. Through the use of reproductive technologies such as estrus synchronization and AI, a greater percentage of females can become bred to superior sires, breeding and calving seasons can be shortened, calf uniformity increased, and economic return maximized (Odde, 1990; Rodgers et al., 2012; …
Static Load Bolted Joint Elastic Resilience Testing Methodology, Paul Melino
Static Load Bolted Joint Elastic Resilience Testing Methodology, Paul Melino
Electronic Theses and Dissertations
Elastic resilience is the ratio of deformation over force an object experiences when under stress. Knowing the how much the bolt and clamped part deform under load is critical when determining fasteners for a bolted joint. VDI 2230 is the internationally recognized bolt sizing standard and contains the equations used to determine each of the bolted joint component’s resilience as well as values for embedment estimates based on component surface roughness. Embedment is the local plastic deformation due to uneven contact over the contact planes of a bolt-clamped interface (Bolt head to plate, plate to plate, plate to nut, and …
Influence Of Sporulation And Germination Behavior Of Bacillus Licheniformis On Microbial Quality Of Skim Milk Powder, Nancy Awasti
Influence Of Sporulation And Germination Behavior Of Bacillus Licheniformis On Microbial Quality Of Skim Milk Powder, Nancy Awasti
Electronic Theses and Dissertations
This dissertation includes modeling of sporulation and germination behavior of Bacillus licheniformis strains during raw milk holding, tracking the survival of sporeformers and spores of high sporulating Bacillus strain during skim milk powder manufacturing. In addition, preliminary studies were done to standardize a rapid spore detection technique by using ratiometric fluorescence. To understand the role of strain variability during raw milk holding conditions, population dynamics of two strains of Bacillus licheniformis, ATCC 6634 and 14580, were modeled as a function of temperature (4.0 -12.0˚ C) and duration (0 - 72 h), using regression analysis. Based on initial spiking of vegetative …
Effects Of Resistant Starch Type 4 On Gut Microbiota Derived Medium And Long Chain Fatty Acids And Bile Acids, Elizabeth Nagel
Effects Of Resistant Starch Type 4 On Gut Microbiota Derived Medium And Long Chain Fatty Acids And Bile Acids, Elizabeth Nagel
Electronic Theses and Dissertations
The human gastrointestinal tract is populated by a diverse and mutualistic microbial flora that has profound effect on human health. Identification and characterization of microbial-metabolites produced in response to human dietary intakes may provide insights into host-microbiota interactions to better understand the role of the microbiota in human health and diseases. However, much of this interaction is poorly understood as many metabolites remain unidentified or understudied at the present time. Starch is ubiquitously present in human diet and is a major energy source to all living cells. Resistant starch (RS) is the starch portion that is not absorbed in the …
Empirical Absolute Calibration Model For Multiple Pseudo-Invariant Calibration Sites, Bipin Raut
Empirical Absolute Calibration Model For Multiple Pseudo-Invariant Calibration Sites, Bipin Raut
Electronic Theses and Dissertations
This work extends an empirical absolute calibration model initially developed for the Libya 4 Pseudo-Invariant Calibration Site (PICS) to five additional Saharan Desert PICS (Egypt 1, Libya 1, Niger 1, Niger 2, and Sudan 1), and demonstrates the efficacy of the resulting models at predicting sensor top-of-atmosphere (TOA) reflectance. It attempts to generate absolute calibration models for these PICS that have an accuracy and precision comparable to or better than the current Libya 4 model, with the intent of providing additional opportunities for sensor calibration. In addition, this work attempts to validate the general applicability of the model to other …
Thiol Specific Fluorogenic Agents For Live Cell Subcellular Organelle Thiol Imaging, Yahya Alqahtani
Thiol Specific Fluorogenic Agents For Live Cell Subcellular Organelle Thiol Imaging, Yahya Alqahtani
Electronic Theses and Dissertations
Thiols or sulfhydryl groups (-SH) play a significant role in protein structures and functions. The unique properties of a thiol group are the basis for its roles in cellular functions. Structurally, thiols are divided into protein thiols (PSH) and non-protein thiols (NPSH). NPSH include glutathione, cysteine, and homocysteine. Thiols are distributed in an uneven manner in the cell and subcellular organelles. Disturbance of thiol levels has been associated with serious diseases such as cancer, aging, cardiovascular diseases, Alzheimer disease, liver damage, and edema. The significant roles of thiols in the biological system have stimulated the development of various approaches to …