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Articles 151 - 169 of 169
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Comparing Effects Of Insecticides On Two Green Lacewings Species, Chrysoperla Johnsoni And Chrysoperla Carnea (Neuroptera: Chrysopidae), K. G. Amarasekare, P. W. Shearer
Comparing Effects Of Insecticides On Two Green Lacewings Species, Chrysoperla Johnsoni And Chrysoperla Carnea (Neuroptera: Chrysopidae), K. G. Amarasekare, P. W. Shearer
Agricultural and Environmental Sciences Faculty Research
This study compared lethal and sublethal effects of five insecticides, chlorantraniliprole, cyantraniliprole, spinetoram, novaluron, and lambda-cyhalothrin, on adult and second instars of two green lacewing species, Chrysoperla carnea (Stephens) and Chrysoperla johnsoni Henry, Wells and Pupedis (Neuroptera: Chrysopidae) in the laboratory. Formulated pesticides were tested using concentrations equivalent to the high label rate dissolved in 378.5 liters of water. Novaluron and lambda-cyhalothrin were toxic to larvae and no treated larvae survived to the adult stage. Larva to adult survival was reduced in chlorantraniliprole, cyantraniliprole, and spinetoram treatments. Larva to adult developmental time and sex ratio were not different among the …
Laboratory Bioassays To Estimate The Lethal And Sublethal Effects Of Various Insecticides And Fungicides On Deraeocoris Brevis (Hemiptera: Miridae), K. G. Amarasekare, P. W. Shearer
Laboratory Bioassays To Estimate The Lethal And Sublethal Effects Of Various Insecticides And Fungicides On Deraeocoris Brevis (Hemiptera: Miridae), K. G. Amarasekare, P. W. Shearer
Agricultural and Environmental Sciences Faculty Research
This laboratory bioassay focused on lethal and sublethal effects of five insecticides (chlorantraniliprole, cyantraniliprole, spinetoram, novaluron, and lambda-cyhalothrin) and two fungicide treatments (sulfur and a mixture of copper hydroxide and mancozeb) on the predatory mired bug, Deraeocoris brevis (Uhler) (Hemiptera: Miridae) using second instars and adult males and females. Formulated pesticides were tested using concentrations that were equivalent to the high label rate (1x) (high rate) and 1/10th of that amount (0.1x) (low rate) dissolved in 378.5 liters of water. Lambda-cyhalothrin was highly toxic to D. brevis nymphs and adults at both rates, whereas both rates of novaluron were highly …
Phenotyping Common Beans For Adaptation To Drought, Stephen E. Beebe, Idupulapati M. Rao, Matthew W. Blair, Jorge A. Acosta-Gallegos
Phenotyping Common Beans For Adaptation To Drought, Stephen E. Beebe, Idupulapati M. Rao, Matthew W. Blair, Jorge A. Acosta-Gallegos
Agricultural and Environmental Sciences Faculty Research
Common beans (Phaseolus vulgaris L.) originated in the New World and are the grain legume of greatest production for direct human consumption. Common bean production is subject to frequent droughts in highland Mexico, in the Pacific coast of Central America, in northeast Brazil, and in eastern and southern Africa from Ethiopia to South Africa. This article reviews efforts to improve common bean for drought tolerance, referring to genetic diversity for drought response, the physiology of drought tolerance mechanisms, and breeding strategies. Different races of common bean respond differently to drought, with race Durango of highland Mexico being a major …
Differential Root Proteome Expression In Tomato Genotypes With Contrasting Drought Tolerance Exposed To Dehydration, Suping Zhou, Marsha Palmer, Jing Zhou, Sarabjit Bhatti, Kevin J. Howe, Tara Fish, Theodore W. Thannhauser
Differential Root Proteome Expression In Tomato Genotypes With Contrasting Drought Tolerance Exposed To Dehydration, Suping Zhou, Marsha Palmer, Jing Zhou, Sarabjit Bhatti, Kevin J. Howe, Tara Fish, Theodore W. Thannhauser
Agricultural and Environmental Sciences Faculty Research
A comparative proteomics study using isobaric tags for relative and absolute quantitation (iTRAQ) was performed on a mesophytic tomato (Solanum lycopersicum) cultivar and a dehydration-resistant wild species (Solanum chilense) to identify proteins that play key roles in tolerance to water deficit stress. In tomato ‘Walter’ LA3465, 130 proteins were identified, of which 104 (80%) were repressed and 26 (20%) were induced. In S. chilense LA1958, a total of 170 proteins were identified with 106 (62%) repressed and 64 (38%) induced. According to their putative molecular functions, the differentially expressed proteins belong to the following subgroups: stress …
Developmental Time, Longevity, And Lifetime Fertility Of Three Introduced Parasitoids Of The Mealybug Paracoccus Marginatus (Hemiptera: Pseudococcidae), Kaushalya G. Amarasekare, Catharine M. Mannion, Nancy D. Epsky
Developmental Time, Longevity, And Lifetime Fertility Of Three Introduced Parasitoids Of The Mealybug Paracoccus Marginatus (Hemiptera: Pseudococcidae), Kaushalya G. Amarasekare, Catharine M. Mannion, Nancy D. Epsky
Agricultural and Environmental Sciences Faculty Research
Developmental time, longevity, and lifetime fertility of three previously introduced parasitoids (Acerophagus papayae Noyes and Schauff, Anagyrus loecki Noyes and Menezes, and Pseudleptomastix mexicana Noyes and Schauff) (Hymenoptera: Encyrtidae) of the mealybug Paracoccus marginatus Williams and Granara de Willink (Hemiptera: Pseudococcidae) were studied in the laboratory to understand the outcome of their recovery in field studies conducted in the United States. The developmental time of both male and female A. papayae and A. loecki was shorter than the developmental time of male and female P. mexicana. Male parasitoids of all three species had a shorter developmental time than …
Aggregation Behavior Of The Southern Chinch Bug (Hemiptera: Blissidae), Karla M. Addesso, Heather J. Mcauslane, Ron Cherry
Aggregation Behavior Of The Southern Chinch Bug (Hemiptera: Blissidae), Karla M. Addesso, Heather J. Mcauslane, Ron Cherry
Agricultural and Environmental Sciences Faculty Research
The southern chinch bug, Blissus insularis Barber, forms dense, multigenerational aggregations in St. Augustinegrass lawns leading to grass death from sap feeding. We conducted laboratory bioassays to better understand the signals responsible for the formation and maintenance of southern chinch bug aggregations. In small arena assays, chinch bugs demonstrated a stronger aggregation response over time and aggregated more often on or beneath St. Augustinegrass leaf blades than on or under artificial leaf-like shelters constructed from white or green paper. In Y-tube olfactometer assays, bugs of different age and sex were attracted to volatiles from mixed-sex chinch bug aggregations and showed …
‘Ramata’: A New Dwarf Variegated Hedychium (Ornamental Ginger) Cultivar, Hamidou F. Sakhanokho, Anthony L. Witcher, Cecil T. Pounders, James M. Spiers
‘Ramata’: A New Dwarf Variegated Hedychium (Ornamental Ginger) Cultivar, Hamidou F. Sakhanokho, Anthony L. Witcher, Cecil T. Pounders, James M. Spiers
Agricultural and Environmental Sciences Faculty Research
Most Hedychium J. Koenig (Zingiberaceae) species have showy and fragrant flowers, which are used in leis and perfumery. Hedychium species are easy to grow and are mostly free of major diseases and pests. The essential oils of some species have been found to have insecticidal, antibacterial, and antifungal activities (Gopanraj et al., 2005; Jadhav et al., 2007; Medeiros et al., 2003). Hedychium plants prefer moist, fertile soil and are considered to be shade plants but will thrive in full sun if provided adequate moisture, and many species will grow and bloom throughout USDA zone 7 (Adams, 1999). Hedychium plants are …
Life History Of An Exotic Soft Scale Insect Phalacrococcus Howertoni (Hemiptera: Coccidae) Found In Florida, Kaushalya G. Amarasekare, Catharine M. Mannion
Life History Of An Exotic Soft Scale Insect Phalacrococcus Howertoni (Hemiptera: Coccidae) Found In Florida, Kaushalya G. Amarasekare, Catharine M. Mannion
Agricultural and Environmental Sciences Faculty Research
We investigated the life history of an exotic soft scale insect, Phalacrococcus howertoni Hodges and Hodgson that damages croton and several other economically important ornamental and fruit plants in Florida. There was no difference in the development, survival, and reproduction of this scale insect species when reared either on croton or on buttonwood at 27 ± 1°C, 12:12 (L:D) h and 65% R. H. The scale insect practiced ovoviviparity. We observed that eggs that came out from vulva hatched to first instars immediately. Newly hatched nymphs had a tendency stay underneath the female body for somewhat less than approximately 24 …
Identification Of Salt-Induced Changes In Leaf And Root Proteomes Of The Wild Tomato, Solanum Chilense, Suping Zhou, Roger J. Sauvé, Zong Liu, Sasikiran Reddy, Sarabjit Bhatti, Simon D. Hucko, Tara Fish, Theodore W. Thannhauser
Identification Of Salt-Induced Changes In Leaf And Root Proteomes Of The Wild Tomato, Solanum Chilense, Suping Zhou, Roger J. Sauvé, Zong Liu, Sasikiran Reddy, Sarabjit Bhatti, Simon D. Hucko, Tara Fish, Theodore W. Thannhauser
Agricultural and Environmental Sciences Faculty Research
This article reports salt-induced changes in leaf and root proteomes after wild tomato (Solanum chilense) plants were treated with 200 mmNaCl. In leaf tissues, a total of 176 protein spots showed significant changes (P < 0.05), of which 104 spots were induced and 72 spots suppressed. Salt-induced proteins are associated with the following pathways: photosynthesis, carbohydrate metabolism, glyoxylate shunt, glycine cleavage system, branched-chain amino acid biosynthesis, protein folding, defense and cellular protection, signal transduction, ion transport, and antioxidant activities. Suppressed proteins belong to the following categories: oxidative phosphorylation pathway, photorespiration and protein translational machinery, oxidative stress, and ATPases. In root tissues, 106 protein spots changed significantly (P< 0.05) after the salt treatment, 63 spots were induced, and 43 suppressed by salt treatment. Salt-induced proteins are associated with the following functional pathways: regeneration of S-adenosyl methionine, protein folding, selective ion transport, antioxidants and defense mechanism, signal transduction and gene expression regulation, and branched-chain amino acid synthesis. Salt-suppressed proteins are receptor kinase proteins, peroxidases and germin-like proteins, malate dehydrogenase, and glycine dehydrogenase. In this study, different members of proteins were identified from leaf and root tissues after plants were subjected to salt treatment. These proteins represent …
Identification Of Alternaria Alternata As A Causal Agent For Leaf Blight In Syringa Species, Margaret T. Mmbaga, Ainong Shi, Mee-Sook Kim
Identification Of Alternaria Alternata As A Causal Agent For Leaf Blight In Syringa Species, Margaret T. Mmbaga, Ainong Shi, Mee-Sook Kim
Agricultural and Environmental Sciences Faculty Research
While many isolates of Alternaria alternata are common saprophytes on trees and shrubs, this study clearly demonstrated that A. alternata is a primary pathogen in lilac (Syringa sp.), causing a leaf-blight that affects different Syringa species. Isolates of Alternaria sp. were collected from leaf blight samples of lilacs in the field. The internal transcribed spacer (ITS) region and morphological characterization were used to identify lilac blight pathogen. Based on 100% ITS nucleotide sequence identities to the Alternaria genus in the GenBank and morphological features, these isolates were identified as A. alternata. Disease symptoms were reproduced in lilac plants inoculated with …
Heat-Induced Proteome Changes In Tomato Leaves, Suping Zhou, Roger J. Sauvé, Zong Liu, Sasikiran Reddy, Sarabjit Bhatti, Simon D. Hucko, Yang Yong, Tara Fish, Theodore W. Thannhauser
Heat-Induced Proteome Changes In Tomato Leaves, Suping Zhou, Roger J. Sauvé, Zong Liu, Sasikiran Reddy, Sarabjit Bhatti, Simon D. Hucko, Yang Yong, Tara Fish, Theodore W. Thannhauser
Agricultural and Environmental Sciences Faculty Research
Three tomato (Solanum lycopersicum) cultivars [Walter LA3465 (heat-tolerant), Edkawi LA 2711 (unknown heat tolerance, salt-tolerant), and LA1310 (cherry tomato)] were compared for changes in leaf proteomes after heat treatment. Seedlings with four fully expanded leaves were subjected to heat treatment of 39/25 °C at a 16:8 h light–dark cycle for 7 days. Leaves were collected at 1200 hr, 4 h after the light cycle started. For ‘Walter’ LA3465, heat-suppressed proteins were geranylgeranyl reductase, ferredoxin-NADP (+) reductase, Rubisco activase, transketolase, phosphoglycerate kinase precursor, fructose–bisphosphate aldolase, glyoxisomal malate dehydrogenase, catalase, S-adenosyl-L-homocysteine hydrolase, and methionine synthase. Two enzymes were induced, cytosolic …
Prunus Domestica Pathogenesis-Related Protein-5 Activates The Defense Response Pathway And Enhances The Resistance To Fungal Infection, Ashraf El-Kereamy, Islam El-Sharkawy, Rengasamy Ramamoorthy, Ali Taheri, Deena Errampalli, Prakash Kumar, Subramanian Jayasankar
Prunus Domestica Pathogenesis-Related Protein-5 Activates The Defense Response Pathway And Enhances The Resistance To Fungal Infection, Ashraf El-Kereamy, Islam El-Sharkawy, Rengasamy Ramamoorthy, Ali Taheri, Deena Errampalli, Prakash Kumar, Subramanian Jayasankar
Agricultural and Environmental Sciences Faculty Research
Pathogenesis-related protein-5 (PR-5) has been implicated in plant disease resistance and its antifungal activity has been demonstrated in some fruit species. However, their roles, especially their interactions with the other defense responses in plant cells, are still not fully understood. In this study, we have cloned and characterized a new PR-5 cDNA named PdPR5-1 from the European plum (Prunus domestica). Expression of PdPR5-1 was studied in different cultivars varying in resistance to the brown rot disease caused by the necrotrophic fungus Monilinia fructicola. In addition transgenic Arabidopsis, ectopically expressing PdPR5-1 was used to study its role in other plant defense …
Host Instar Susceptibility And Selection And Interspecific Competition Of Three Introduced Parasitoids Of The Mealybug Paracoccus Marginatus (Hemiptera: Pseudococcidae), Kaushalya G. Amarasekare, Catharine M. Mannion, Nancy D. Epsky
Host Instar Susceptibility And Selection And Interspecific Competition Of Three Introduced Parasitoids Of The Mealybug Paracoccus Marginatus (Hemiptera: Pseudococcidae), Kaushalya G. Amarasekare, Catharine M. Mannion, Nancy D. Epsky
Agricultural and Environmental Sciences Faculty Research
Three previously introduced parasitoids (Acerophagus papayae Noyes and Schauff, Anagyrus loecki Noyes and Menezes, and Pseudleptomastix mexicana Noyes and Schauff [Hymenoptera: Encyrtidae]) of the mealybug Paracoccus marginatus Williams and Granara de Willink (Hemiptera: Pseudococcidae) were studied for their host instar susceptibility and sex ratio, host instar selection, and interspecific competition in the laboratory. All three parasitoids were able to develop in the second instars, third-instar females, and adult females of P. marginatus. No progeny emerged from first-instar mealybugs. The proportion of female emergence was increased with increasing host size. Parasitoids selected their host instars for oviposition when they …
Effects Of Organic And Inorganic Fertilizers On Marigold Growth And Flowering, Guihong Bi, William B. Evans, James M. Spiers, Anthony L. Witcher
Effects Of Organic And Inorganic Fertilizers On Marigold Growth And Flowering, Guihong Bi, William B. Evans, James M. Spiers, Anthony L. Witcher
Agricultural and Environmental Sciences Faculty Research
Two experiments were conducted to evaluate the growth and flowering responses of greenhouse-grown French marigold (Tagetes patula L. ‘Janie Deep Orange’) to two non-composted broiler chicken litter-based organic fertilizers, 4-2-2 and 3-3-3, and one commonly used synthetic controlled-release fertilizer, 14-14-14. In both experiments, fertilizer 4-2-2 was applied at four rates of 1%, 2%, 4%, and 6% (by volume); 3-3-3 was applied at four rates of 1.34%, 2.67%, 5.34%, and 8.0% (by volume); and 14-14-14 was applied at rates of 0.99, 1.98, 3.96, and 5.94 kg·m−3. In general, substrate containing different rates and types of fertilizers had a pH within the …
Salt-Induced And Salt-Suppressed Proteins In Tomato Leaves, Suping Zhou, Roger Sauve, Tara Fish, Theodore W. Thannhauser
Salt-Induced And Salt-Suppressed Proteins In Tomato Leaves, Suping Zhou, Roger Sauve, Tara Fish, Theodore W. Thannhauser
Agricultural and Environmental Sciences Faculty Research
Tomato (Solanum lycopersicum cv. Money Maker) seedlings at the two-leaf stage were grown in one-half strength Hoagland solution supplemented with 50 mm NaCl for 4 days, with 100 mm NaCl for 4 days, with 150 mm NaCl for 4 days, and with a final concentration 200 mm NaCl for 2 days. Solutions were refreshed every 2 days for treated and untreated seedlings. Non-treated plants were grown in nonamended one-half strength Hoagland solution. Three biological replicates (BR) were included for treated and control experiments. At the end of treatments, the uppermost three newly expanded leaves from all 12 plants in each …
Wholetree Substrate And Fertilizer Rate In Production Of Greenhouse-Grown Petunia (Petunia ×Hybrida Vilm.) And Marigold (Tagetes Patula L.), Glenn B. Fain, Charles H. Gilliam, Jeff L. Sibley, Cheryl R. Boyer, Anthony L. Witcher
Wholetree Substrate And Fertilizer Rate In Production Of Greenhouse-Grown Petunia (Petunia ×Hybrida Vilm.) And Marigold (Tagetes Patula L.), Glenn B. Fain, Charles H. Gilliam, Jeff L. Sibley, Cheryl R. Boyer, Anthony L. Witcher
Agricultural and Environmental Sciences Faculty Research
A substrate component (WholeTree) made from loblolly pine (Pinus taeda L.) was evaluated along with starter fertilizer rate in the production of greenhouse-grown petunia (Petunia ×hybrida Vilm. ‘Dreams Purple’) and marigold (Tagetes patula L. ‘Hero Spry’). Loblolly pine from a 12-year-old plantation were harvested at ground level, chipped, and further processed through a hammer mill to pass a 0.64-cm screen. The resulting WholeTree (WT) substrate was used alone or combined with 20% (WTP2) or 50% (WTP5) (by volume) Canadian sphagnum peatmoss and compared with an industry standard peat-lite (PL) mix of 8 peatmoss : 1 vermiculite : 1 perlite (by …
Analysis Of Powdery Mildew-Resistant Dogwood Accessions Using Aflp, Margaret T. Mmbaga, Roger J. Sauvé
Analysis Of Powdery Mildew-Resistant Dogwood Accessions Using Aflp, Margaret T. Mmbaga, Roger J. Sauvé
Agricultural and Environmental Sciences Faculty Research
Twenty-five dogwood accessions (one Cornus kousa, three C. kousa × C. florida hybrids, and 21 C. florida) were characterized using amplified fragment length polymorphism. Among the C. florida accessions, four were named cultivars and 17 were selections from Tennessee State University's dogwood breeding program. Amplified fragment length polymorphism band profiles obtained from 13 EcoRI/MseI (+3/+3) primer pairs showed the presence of high genetic diversity between species and within the C. florida accessions. Each accession was distinctly different from each other, and the resistant clones clustered into distinct groups.
Structure And Temperature Regulated Expression Of A Cysteine Proteinase Gene In Pachysandra Terminalis Sieb. & Zucc., Suping Zhou, Roger Sauve, Fur-Chi Chen
Structure And Temperature Regulated Expression Of A Cysteine Proteinase Gene In Pachysandra Terminalis Sieb. & Zucc., Suping Zhou, Roger Sauve, Fur-Chi Chen
Agricultural and Environmental Sciences Faculty Research
A cysteine proteinase gene (DQ403257) with an open reading frame of 1125 base pairs was isolated from Pachysdandra terminalis. The primary translated peptide has a predicted length of 374 amino acids, pI (isoelectric point) of 5.70, and molecular mass of 40.9 kDa. The Peptidase_C1 domain is between residue 141 and 367. The proteinase has a conserved motif Gly-Xaa-Thy-Xaa-Phe-Xaa-Asn in the pro region. Sequence comparison shows that the deduced peptide shares 82% identity with the cysteine proteinase RD19a precursor (RD19) (accession P43296) from Arabidopsis thaliana (L.) Heynh. Real-time quantitative reverse-transcriptase–polymerase chain reaction revealed that the gene is induced by treatments of …
Identification Of Poinsettia Cultivars Using Rapd Markers, Jing-Tian Ling, Roger Sauve, Nick Gawel
Identification Of Poinsettia Cultivars Using Rapd Markers, Jing-Tian Ling, Roger Sauve, Nick Gawel
Agricultural and Environmental Sciences Faculty Research
Randomly amplified polymorphic DNA (RAPD) techniques were used to compare the DNA from leaf tissues of nine commercial poinsettia (Euphorbia pulcherrima Wild ex Klotzsch) cultivars. Amplification occurred in 57 out of 60 (95%) tested primers. Nine primers that revealed polymorphisms among cultivars were selected for further evaluation. Forty-eight RAPD bands were scored from these primers, and 33 (69%) were polymorphic. All tested cultivars could be discriminated with seven bands generated from primers OPB7 and OPC13. Results of a UPGMA cluster analysis and principal components analysis placed the nine cultivars into two groups: one group consisted of `Jingle Bells', `Supjibi', …