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Plant Sciences

Agricultural and Environmental Sciences Faculty Research

Gene expression

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Differentially Expressed Genes In Resistant And Susceptible Common Bean (Phaseolus Vulgaris L.) Genotypes In Response To Fusarium Oxysporum F. Sp. Phaseoli, Renfeng Xue, Jing Wu, Zhendong Zhu, Lanfen Wang, Xiaoming Wang, Shumin Wang, Matthew W. Blair Jun 2015

Differentially Expressed Genes In Resistant And Susceptible Common Bean (Phaseolus Vulgaris L.) Genotypes In Response To Fusarium Oxysporum F. Sp. Phaseoli, Renfeng Xue, Jing Wu, Zhendong Zhu, Lanfen Wang, Xiaoming Wang, Shumin Wang, Matthew W. Blair

Agricultural and Environmental Sciences Faculty Research

Fusarium wilt of common bean (Phaseolus vulgaris L.), caused by Fusarium oxysporum Schlechtend.:Fr. f.sp. phaseoli (Fop), is one of the most important diseases of common beans worldwide. Few natural sources of resistance to Fop exist and provide only moderate or partial levels of protection. Despite the economic importance of the disease across multiple crops, only a few of Fop induced genes have been analyzed in legumes. Therefore, our goal was to identify transcriptionally regulated genes during an incompatible interaction between common bean and the Fop pathogen using the cDNA amplified fragment length polymorphism (cDNA-AFLP) technique. We generated a …


Comparison Of Five Major Trichome Regulatory Genes In Brassica Villosa With Orthologues Within The Brassicaceae, Naghabushana K. Nayidu, Sateesh Kagale, Ali Taheri, Thushan S. Withana-Gamage, Isobel A. P. Parkin, Andrew G. Sharpe, Margaret Y. Gruber Apr 2014

Comparison Of Five Major Trichome Regulatory Genes In Brassica Villosa With Orthologues Within The Brassicaceae, Naghabushana K. Nayidu, Sateesh Kagale, Ali Taheri, Thushan S. Withana-Gamage, Isobel A. P. Parkin, Andrew G. Sharpe, Margaret Y. Gruber

Agricultural and Environmental Sciences Faculty Research

Coding sequences for major trichome regulatory genes, including the positive regulators GLABRA 1(GL1), GLABRA 2 (GL2), ENHANCER OF GLABRA 3 (EGL3), and TRANSPARENT TESTA GLABRA 1 (TTG1) and the negative regulator TRIPTYCHON (TRY), were cloned from wild Brassica villosa, which is characterized by dense trichome coverage over most of the plant. Transcript (FPKM) levels from RNA sequencing indicated much higher expression of the GL2 and TTG1 regulatory genes in B. villosa leaves compared with expression levels of GL1 and EGL3 genes in either B. villosa or the reference genome species, glabrous B. oleracea; however, cotyledon TTG1 expression was high in …


The Phaseolus Vulgaris Zip Gene Family: Identification, Characterization, Mapping, And Gene Expression, Carolina Astudillo, Andrea C. Fernandez, Matthew W. Blair, Karen A. Cichy Jul 2013

The Phaseolus Vulgaris Zip Gene Family: Identification, Characterization, Mapping, And Gene Expression, Carolina Astudillo, Andrea C. Fernandez, Matthew W. Blair, Karen A. Cichy

Agricultural and Environmental Sciences Faculty Research

Zinc is an essential mineral for humans and plants and is involved in many physiological and biochemical processes. In humans, Zn deficiency has been associated with retarded growth and reduction of immune response. In plants, Zn is an essential component of more than 300 enzymes including RNA polymerase, alkaline phosphatase, alcohol dehydrogenase, Cu/Zn superoxidase dismutase, and carbonic anhydrase. The accumulation of Zn in plants involves many genes and characterization of the role of these genes will be useful in biofortification. Here we report the identification and phlyogenetic and sequence characterization of the 23 members of the ZIP (ZRT, IRT like …