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Portable X-Ray Fluorescence Spectrometry For Sensing Salinity And Sodicity In Glacial Northern Great Plains Soils With Machine Learning Models, Adam Devlin
Electronic Theses and Dissertations
Saline and sodic soils are an increasing concern across the Northern Great Plains (NGP) due to factors of climate change and land management that are drawing geologically derived salts to the land surface. Traditional laboratory assessments, such as electrical conductivity (EC) and sodium adsorption ratio (SAR), are time and resource consumptive. Portable X-ray fluorescence (PXRF) may be a viable proximal sensing alternative, as it is able to provide elemental data in minutes, in situ or ex situ, and can directly quantify salinity-associated elements like Ca, Mg, and S. PXRF paired with predictive models has proven useful for a range of …
Developing Machine Learning Models For Selection Of Management Zones, Sravanthi Bachina
Developing Machine Learning Models For Selection Of Management Zones, Sravanthi Bachina
Electronic Theses and Dissertations
Soil sampling and analyses play a crucial role in optimizing nutrient management and enhancing crop productivity. However, collecting representative samples across diverse landscapes is challenging due to knowledge gaps about spatial variability of soil properties, large fields, multiple samples, and analysis costs. Collecting soil samples based on the management zones can help farmers gather precise information about soil properties with fewer samples. Recent developments in precision agriculture and machine learning. This study aimed to develop machine learning models that can learn, analyze, and refine landscape and soil properties data for automated selection of soil sampling zones and generating prediction maps. …
The Hutton Project: Long-Term Agricultural Impacts On Soil Loss And Carbon Dynamics In Eastern South Dakota, Eli Halverson
The Hutton Project: Long-Term Agricultural Impacts On Soil Loss And Carbon Dynamics In Eastern South Dakota, Eli Halverson
Electronic Theses and Dissertations
Long-term and intensified agricultural land management has resulted in increased rates of soil erosion and has altered much of the carbon cycle at regional and global scales. Anthropogenic degradation of soil resources is a barrier to sustainable production, soil functioning, and ecosystem services. It is difficult to quantify the scope of pedogenic changes due to the lack of legacy data and short temporal scales. This study utilized decades to century-old soil information to quantify historical soil erosion losses and changes in soil carbon pools of eastern South Dakota soils. The results show that soils in the region have been significantly …
Utilizing Soil Sensors To Assess Soil Health And Investigating Cover Crops Impact On Methane Emission, Shailesh Pandit
Utilizing Soil Sensors To Assess Soil Health And Investigating Cover Crops Impact On Methane Emission, Shailesh Pandit
Electronic Theses and Dissertations
Soil health is influenced by climate change, cultural practices, topography, crop rotations, cover crops, soil parent materials, and soil biota. Interactions between these factors can improve or reduce soil health. This thesis investigates two topics, using soil sensors to provide a rapid assessment of soil health, and the impact of cover crops on soil biological activity and greenhouse gas emissions. Chapter 1 explores the use of two types of sensors to measure apparent electrical conductivity (ECa) and provides examples on the use of these sensors. Even though both sensors measure ECa using different processes, they provide useful information about temporal …
Integrating Early Season Clover Cover Crops As A Living Mulch In Broccoli And Transitional Organic Winter Squash Production In Eastern South Dakota, Alexis Rose Barnes
Integrating Early Season Clover Cover Crops As A Living Mulch In Broccoli And Transitional Organic Winter Squash Production In Eastern South Dakota, Alexis Rose Barnes
Electronic Theses and Dissertations
Managing weeds, improving soil health, and reducing the use of plastic mulch continue to be priorities for South Dakota vegetable farmers. Farmers have expressed an interest in integrating cover crops into their farm systems. Clover cover crops used as a living mulch within and along cash crop rows may aid in weed suppression, nitrogen fixation, and prevent soil erosion. However, prior research has shown challenges of incorporating living mulch due to yield decreases. Research conducted in 2022 and 2023 in eastern South Dakota investigated the effects of four clover and four in-row soil management treatments on broccoli and organic winter …
Nitrogen Application Timing And Cereal Rye (Secale Cereale L.) Cover Crop Influence Greenhouse Gas Emissions, Graig Reicks
Nitrogen Application Timing And Cereal Rye (Secale Cereale L.) Cover Crop Influence Greenhouse Gas Emissions, Graig Reicks
Electronic Theses and Dissertations
Dryland contributions to greenhouse gas (GHG) emissions are increased by N fertilizer applications and high soil water contents. Fertilizer timing and spring growth of cover crops prior to cash crop planting were investigated in separate studies to examine impacts on overall GHG emissions using a near continuous measurement system. There was a significant interaction between N fertilizer rate (0 vs. 224 kg N ha-1 surface-applied as urea) and application date (p= 0.01) for CO2 emissions. This interaction occurred because N fertilizer increased CO2 emissions by 35% for the 21 d interval following early spring application. When application was delayed until …
Identifying Optimum Germination Temperatures And Analysis Weights In Seed Testing For Stiff Goldenrod (Solidago Rigida) And River Bulrush (Bolboschoenus Fluviatilis), Rachel Geary
Electronic Theses and Dissertations
Federal and state seed laws require seed lots offered for sale to be tested and labeled with the appropriate information before they can be sold. Those tests are conducted in seed testing labs all around the world. In the United States, accredited seed testing labs follow the Association of Official Seed Analysts (AOSA) Rules for Testing Seeds to provide information for labeling. The rules lack instructions for weights and germination for many native species. Stiff goldenrod (Solidago rigida) and river bulrush (Bolboschoenus fluviatilis) are two species that lack any information required for testing. When seed testing labs use correct weights …
Tracking Nitrogen Mineralization In The Presence Of Biochar Utilizing Complementary-Method Assays In East Central South Dakota, Andrew Calvin Engel
Tracking Nitrogen Mineralization In The Presence Of Biochar Utilizing Complementary-Method Assays In East Central South Dakota, Andrew Calvin Engel
Electronic Theses and Dissertations
Understanding soil N transformations in agricultural systems of the Northern Great Plains is crucial for guiding effective soil and nutrient management of this vital cropland. While the use of biochar in agriculture has attracted great attention recently, little has been reported on the seasonal dynamics of soil nitrogen (N) transformation and its response to biochar application. A field experiment was conducted over 2 seasons, spring (6/3/19-6/24/19) and summer (7/26/19-8/16/19), using four treatments or control soil, urea (224 kg N/ha), biochar (46,250 kg/ha), and urea+biochar (224 kg N/ha and 46,250 kg/ha, respectively). The results for both seasons showed biochar had no …
Enhancing Nitrogen Use Efficiency Through Ai-Powered Image Analysis And Innovative N-Rich Spot Method, Bobby Azad
Enhancing Nitrogen Use Efficiency Through Ai-Powered Image Analysis And Innovative N-Rich Spot Method, Bobby Azad
Electronic Theses and Dissertations
This study conducted in 2023 aimed to enhance nitrogen use efficiency (NUE) in wheat and corn grown in South Dakota. Based on dynamic weather conditions and other factor interactions, conventional nitrogen (N) recommendations need to be improved. Soil properties information, including electrical conductivity, was used to create management zones. In each zone, three N-rich spots were established as biosensors. Drones and satellites collected imagery data, and an AI-driven approach assessed the crop response to applied N. A dynamic N application approach, integrating aerial data with historical records, was developed and evaluated. Our methodology, at a 95% confidence level, resulted in …
Unveiling Race Diversity And Fungicide Sensitivity Profiles Of Pyrenophora Tritici Repentis Populations In South Dakota Collected From 2021-2023, Jaswinder Kaur
Unveiling Race Diversity And Fungicide Sensitivity Profiles Of Pyrenophora Tritici Repentis Populations In South Dakota Collected From 2021-2023, Jaswinder Kaur
Electronic Theses and Dissertations
Tan spot, a foliar disease of wheat caused by the ascomycetes fungus Pyrenophora tritici repentis (Ptr), poses a significant threat to wheat crops globally. The disease gained economic importance in the late 1970s due to shifts in farming practices, such as minimal tillage and planting on crop residues, providing favorable conditions for the pathogen to overwinter and cause infections. Ptr is classified into eight races based on the production of necrotrophic effectors—Ptr ToxA (necrosis-inducing), Ptr ToxB, and Ptr ToxC (chlorosis-inducing on respective susceptible and sensitive cultivars)—resulting in distinct symptoms on susceptible wheat differentials. The inverse gene-for-gene hypothesis of Ptr suggests …
Fungicide Sensitivity In P. Tritici-Repentis Diverse Population And Phenotyping Of Spelt Wheat For Multiple Diseases, Zunera Shabbir
Fungicide Sensitivity In P. Tritici-Repentis Diverse Population And Phenotyping Of Spelt Wheat For Multiple Diseases, Zunera Shabbir
Electronic Theses and Dissertations
Pyraclostrobin, a QoI fungicide, is extensively used in the management of fungal leaf spot diseases. In this study, 215 P. tritici-repentis isolates collected from wheat fields in South Dakota were evaluated for their sensitivity to Pyraclostrobin. Of 215 isolates, 48 isolates (22%) exhibited insensitivity based on G143A mutation and EC50 values using spore germination assay. Our results indicate reduced sensitivity to insensitivity to pyraclostrobin in some isolates suggesting regular monitoring of the P. tritici-repentis population to QoI fungicides is essential to track the evolution of insensitive population. Spelt wheat (Triticum spelta L.) is an ancient cereal that is gaining small …
Building No-Tillage Maize Nitrogen Recommendation Algorithm That Considers Improvements In Soil Health, Dwarika Bhattarai
Building No-Tillage Maize Nitrogen Recommendation Algorithm That Considers Improvements In Soil Health, Dwarika Bhattarai
Electronic Theses and Dissertations
No abstract provided.
Methods For Improving Potassium Fertilizer Recommendations For Corn In South Dakota, Andrew J. Ahlersmeyer
Methods For Improving Potassium Fertilizer Recommendations For Corn In South Dakota, Andrew J. Ahlersmeyer
Electronic Theses and Dissertations
Corn (Zea mays L.) is a vital commodity in South Dakota’s agricultural sector. Optimal corn production occurs when there are sufficient mineral nutrients in the soil, especially potassium (K). Applications of K fertilizer are used when soil test K (STK) levels are deficient. Therefore, producers need reliable, thoroughly tested fertilizer recommendations to make profitable decisions and maintain environmental stewardship. South Dakota K fertilizer recommendations have not been updated in nearly 20 years. Simultaneously, changes in corn genetics, management practices, and climate patterns suggest that the critical soil test value (CSTV) for STK may have shifted in that same time frame. …
Improving The Utility Of Precision Agriculture Through Machine Learning And Climate-Smart Practices, Skye Brugler
Improving The Utility Of Precision Agriculture Through Machine Learning And Climate-Smart Practices, Skye Brugler
Electronic Theses and Dissertations
Climate Smart Practices are management strategies that focus on increasing soil and crop productivity, utilize site-specific strategies to increase resiliency against the effects of climate change, and mitigate these negative effects by reducing greenhouse gas (GHG) emissions. Decision Support Systems (DSSs) using machine learning (ML) can adjust models based on new information and help farmers make climate smart decisions within their operation. The 4R nutrient management model of right source, rate, location, and time also demonstrates a framework that may be considered climate smart by improving soil and crop productivity. However, when initially conceptualized, the 4R model did not consider …
Soil Profile Properties And Greenhouse Gas Emissions As Influenced By Long-Term Cattle Manure And Inorganic Fertilizer Applications Under Corn-Soybean-Spring Wheat Rotation In Eastern South Dakota, Anuoluwa Ojonoka Sangotayo
Soil Profile Properties And Greenhouse Gas Emissions As Influenced By Long-Term Cattle Manure And Inorganic Fertilizer Applications Under Corn-Soybean-Spring Wheat Rotation In Eastern South Dakota, Anuoluwa Ojonoka Sangotayo
Electronic Theses and Dissertations
The application of manure and inorganic fertilizer in row crops may significantly influence soil and greenhouse gas (GHG) emissions. Understanding the long-term influence of these management practices on soil pore characteristics, hydro-physical properties and greenhouse gas emission is essential in developing proper conservation practices. However, there is limited information on the impact of cattle manure and inorganic fertilizer application on soil hydro-physical properties, soil pore characteristics at lower depths and surface GHGs emissions. Therefore, the objectives of this study were to; (i) utilize X-ray computed tomography (XCT) technique to quantify the impact of manure and fertilizer amendments under a corn …
Investigating Dormancy And Germination Characteristics To Promote Restoration Success In The Northern Great Plains, Gregory A. Cooper
Investigating Dormancy And Germination Characteristics To Promote Restoration Success In The Northern Great Plains, Gregory A. Cooper
Electronic Theses and Dissertations
Failures in seed-based restoration can be partially attributed to a lack of knowledge on seed dormancy. Dormancy is beneficial for plant establishment in unpredictable environmental conditions, but a lack of uniform germination can hinder restoration efforts. The purpose of this research was to gain a better understanding of dormancy displayed by select forbs of the northern Great Plains. The first data chapter attempted to identify and break dormancy class. Seeds were treated with scarification, smoke, and fertilizer pretreatments in an attempt to break seed dormancy. Seeds were also placed in spring and summer temperatures to identify how seasonal phenology influenced …
Addressing Soil Carbon Sequestration Response From Multispecies Dairy Forage Systems And Modeling Rangeland Beef Cow Dry Matter Intake Using Precision Enteric Emissions Measurements, Lillian J. Mcfadden
Addressing Soil Carbon Sequestration Response From Multispecies Dairy Forage Systems And Modeling Rangeland Beef Cow Dry Matter Intake Using Precision Enteric Emissions Measurements, Lillian J. Mcfadden
Electronic Theses and Dissertations
Regenerative agriculture is a pressing matter for the dairy industry to address cropland sustainability and carbon sequestration. One regenerative management practice that has been identified to help with row crop sustainability for key metrics like soil organic carbon (SOC) is complex covers. When producers use complex covers one of the main challenges is that it takes time to detect a change in SOC. However, simulation models are a tool that can be used to help determine if a regenerative practice is a strategy that gives the best results (i.e., increased SOC) while aligning with long-term production goals. Therefore, our objectives …
The Effects Of Residue Amounts And Variety Selection On The Development Of Wheat Fungal Pathogens In South Dakota, Abrielle Tembreull
The Effects Of Residue Amounts And Variety Selection On The Development Of Wheat Fungal Pathogens In South Dakota, Abrielle Tembreull
Electronic Theses and Dissertations
Roughly 50% of South Dakota cropland is under the practice of no-till, with large increases in acres of no-till in the last 20 or so years. No-till is beneficial for Great Plains states, such as South Dakota, as soil residues are withheld. Residues are shown to increase soil health through retention of moisture and increase of microorganisms, but residues can serve as a source of inoculum for fungal pathogens that can overwinter on them. As wheat is one of the most important food crops in the world, it is important to protect this vital crop from diseases. Diseases of wheat …
Expanding The Use Of High-Performance Liquid Chromatography For Varietal Identification Or Verification Of Selected Cereal Crops, Johnna Jorgensen
Expanding The Use Of High-Performance Liquid Chromatography For Varietal Identification Or Verification Of Selected Cereal Crops, Johnna Jorgensen
Electronic Theses and Dissertations
High-performance liquid chromatography (HPLC) is a form of analytical chemistry where adsorption is a key factor in which the purpose is to separate, identify and quantify each component in a mixture. It is the optimal separation technique of chemical and biological compounds that are non-volatile including proteins and natural products (i.e., plant extracts). As each sample component interacts differently with the absorbent material within the column, the flow rates change leading to the separation of peaks, which are plotted via computer software. Most commonly HPLC analysis has been used for chemical manufacturing and pharmaceutical purposes. When using HPLC for crop …
Genomics-Assisted Approaches To Improve Grain Yield And End-Use Quality In Hard Winter Wheat (Triticum Aestivum L.), Harsimardeep Singh Gill
Genomics-Assisted Approaches To Improve Grain Yield And End-Use Quality In Hard Winter Wheat (Triticum Aestivum L.), Harsimardeep Singh Gill
Electronic Theses and Dissertations
Global wheat production needs to be increased by 60% to meet the future demand of feeding nine billion people by 2050. Simultaneously, it is important to improve the enduse quality to meet the requirements of producers, grain markets, processors, and consumers. Thus, the development of more productive wheat varieties with better enduse quality remains the primary focus for all wheat breeding programs. However, direct phenotypic selection for improving grain yield and end-use quality is difficult as it is highly influenced by environmental factors. This dissertation focuses on harnessing advancements in genomics applications, including genome-wide association studies (GWAS), for the genetic …
Fusarium Head Blight Resistance Assessment In Barley, Tasneem Fathima
Fusarium Head Blight Resistance Assessment In Barley, Tasneem Fathima
Electronic Theses and Dissertations
Fusarium head blight (FHB), commonly called scab is the most devastating disease of wheat and barley caused by the ascomycete fungus, Fusarium graminearum. FHB affects both the quality and quantity of cereal grains affecting the supply chain of the food and feed industry and the marketability of the produce. Barley (Hordeum vulgare L.) is among the several crop species most vulnerable to FHB infection. An estimated 25% of Spring barley grain production in the United States is used to make malted beverages such as beer. Malting barley is a relatively high-value crop with high demands regarding grain quality. FHB directly …
Biological Control Of Bacterial Leaf Streak Of Wheat Using Antagonistic Rhizobacteria, Ashley Frederickson
Biological Control Of Bacterial Leaf Streak Of Wheat Using Antagonistic Rhizobacteria, Ashley Frederickson
Electronic Theses and Dissertations
Wheat (Triticum aestivum L.) is one of the world’s most valuable crops, and the primary food grain produced in the United States. A major disease in wheat production is Bacterial Leaf Streak (BLS) caused by the gram-negative bacterium Xanthomonas translucens pv. undulosa (Xtu). Very few management strategies are available to growers to manage this disease, as common chemicals do not provide adequate control. A number of studies have been conducted on biological control using Rhizospheric bacteria for the control of various plant diseases, but limited data is available on the efficacy of biological control using Rhizospheric bacteria for controlling Bacterial …
Effect Of Foliar And Preplant Sulfur Application On Soybean Nutrient Uptake, Yield, And Seed Protein And Oil Content, Gena Ram Mahato
Effect Of Foliar And Preplant Sulfur Application On Soybean Nutrient Uptake, Yield, And Seed Protein And Oil Content, Gena Ram Mahato
Electronic Theses and Dissertations
In recent years, Sulfur (S) deficiency has been observed in soybean producing regions in the US and different parts of the world. The major factors behind the frequent occurrence of S deficiency are reduced atmospheric S deposition due to strict regulations on emissions from fossil fuels, large S removal from high yielding crops, less use of fertilizers containing S, and intensive cultivation practices. To test S limitations, I conducted two independent studies from 2019 to 2021 at two locations in Eastern South Dakota to determine the effect of foliar and preplant S application on soybean. The objectives of this research …
Phosphate2 And Pin-Likes7 Affect Symbiotic Nodule Phenotypes In Soybean, Bhawandeep Kaur
Phosphate2 And Pin-Likes7 Affect Symbiotic Nodule Phenotypes In Soybean, Bhawandeep Kaur
Electronic Theses and Dissertations
Legumes and soil rhizobia share a symbiotic relationship, wherein rhizobia fix atmospheric dinitrogen in exchange for photosynthates from the host plant. This exchange of mutual benefits occurs in a specialized root structure called ‘nodule’. Soybean (Glycine max) nodules arise from root outer cortical cells. De novo cell differentiation of cortical cells during nodule development leads to the formation of two distinct nodule zones in the mature nodule: the central infection zone where nitrogen fixation occurs and the peripheral parenchyma zone that houses vascular bundles. Plant signaling mechanisms that dictate the development of these nodule zones are not known. To address …
Phomopsis Stem Canker Of Sunflower (Helianthus Annuus L.): Etiology And Epidemiological Conditions Affecting Endophytic Population Of Diaporthe Spp., And Fungicide Efficacy, Nabin Kumar Dangal
Phomopsis Stem Canker Of Sunflower (Helianthus Annuus L.): Etiology And Epidemiological Conditions Affecting Endophytic Population Of Diaporthe Spp., And Fungicide Efficacy, Nabin Kumar Dangal
Electronic Theses and Dissertations
Phomopsis stem canker (PSC), caused by species of Diaporthe, is a major disease of sunflower (Helianthus annuus L.) in the U.S., which can cause yield loss over 40%. The disease prevalence increased since 2010 in the major sunflower producing U.S. states of Nebraska, Minnesota, North Dakota, and South Dakota. Previous studies on the use of foliar fungicides in managing Phomopsis stem canker indicated mixed results on their efficacy in disease reduction and yield gain. Additionally, we do not have sufficient information on asymptomatic colonization by endophytic Diaporthe on sunflower tissue and how foliar fungicide and weather parameters affect the prevalence …
Regenerative Agriculture Effects On Invertebrate And Bird Communities And Insect-Provided Ecosystem Services, Alex Michels
Regenerative Agriculture Effects On Invertebrate And Bird Communities And Insect-Provided Ecosystem Services, Alex Michels
Electronic Theses and Dissertations
Conventional agricultural practices can have unintended consequences on the environment and wildlife. Insects and birds are declining at rapid rates around the world, and the current conventional agricultural paradigm is a major driver through habitat loss and the intensification of production. Invertebrates in agroecosystems provide services to both farmers and the rest of society. Regenerative systems may promote the functioning of an agroecosystem by influencing invertebrate abundance, diversity, and ecosystem services and mitigate bird and insect declines through conservation practices that increase soil health, reduce disturbances, and increase biological diversity. Here I address knowledge gaps of the effects of regenerative …
Identifying Sources Of Resistance And Fungicide Efficacy Against Fungi Causing Phomopsis Stem Canker In Sunflower, Renan Guidini
Identifying Sources Of Resistance And Fungicide Efficacy Against Fungi Causing Phomopsis Stem Canker In Sunflower, Renan Guidini
Electronic Theses and Dissertations
Phomopsis stem canker is a major threat to sunflower (Helianthus annuus L.) production worldwide and its prevalence has increased from 1.5% in 2001 to 61.0% in 2015 in the sunflower producing U.S. states of Colorado, Kansas, Minnesota, Nebraska, North Dakota, South Dakota, and Texas. The disease causes early senescence and lodging of sunflower plants resulting in yield losses up to 40% and oil content up to 25%. Several species of Diaporthe have been reported to cause Phomopsis stem canker. In the United States, Diaporthe helianthi Munyt.-Cvetk. et al. and D. gulyae Shivas et al., have been reported as predominant causal …
Development Of Genomic Resources In Vitis Riparia For Discoveries On Pre- And Post-Transcriptional Molecular Regulators Of Early Induction Into Endodormancy, Michael Robben
Electronic Theses and Dissertations
Grapevine is one of the most important fruit crops in the world, responsible for billions in global sales annually. The largest threat to grapevine and other crop production is global climate change resulting human activities. This brings unpredictable and drastic changes in ambient air temperatures to many climates in which grapes are grown. Lower temperatures and inclement weather are already responsible for millions in lost revenue due to tissue damage of established plants. Thus, protecting grapevine crops from weather-related damage is the biggest concern to growers aside from pathogen- and diseaserelated crop damage. The primary mechanism for winter survival in …
E. Coli Persister Cell Survival And Rhizobia Attachment To Soybean Roots, Tanim Islam
E. Coli Persister Cell Survival And Rhizobia Attachment To Soybean Roots, Tanim Islam
Electronic Theses and Dissertations
The theme of this thesis revolves around how bacteria respond and thrive during stress. Chapters 1-3 are about how bacteria deal with life-threatening antibiotics. Chapter 4 covers new research on how bacteria can move from a stressful individual lifestyle (free-living bacteria) to initiating a symbiotic relationship with a plant (a less stressful lifestyle). In Chapter 1, I briefly summarize the current state of knowledge in the field of antibiotic resistance and persistence. In Chapter 2, I add to this knowledge by providing new insights into several antibiotics' potency and exploring the antibiotic Eagle effect. In Chapter 3, I use pyruvate …
Exploring Bioprocessing Technologies For Diverse Industrial Application Of Canola, Ahmad Fawzi N Alhomodi
Exploring Bioprocessing Technologies For Diverse Industrial Application Of Canola, Ahmad Fawzi N Alhomodi
Electronic Theses and Dissertations
Globally, canola is the second largest oilseed crop after soybean, which is processed for the commercial production of high value oil. The industrial processing steps for canola oil extraction include preprocessing (cleaning, pressing, flaking, and cooking), mechanical pressing, and/or organic solvents. This process results in large quantities of protein-rich (~ 40% dry basis) meal as a co-product. The meal is used partially in animal diet (<30% inclusion) due to high levels of antinutritional factors (ANFs) such as high fibers, phytic acid, and glucosinolates, and low metabolizable energy. Thus, this research was designed to examine various bioprocessing technologies (i.e., traditional sprouting, solid state fermentation, submerged state fermentation, co-culture fermentation, mild pretreatments, and their combinations) for possible advancement in canola use. Canola seed sprouting for 6-day period led to an increase in protein content and a reduction in ANFs and oil content of sprouts compared to ungerminated seed. Subsequent submerged state fermentation of 6- day old sprouts using three different strains (Aureobasidium pullulans, Trichoderma reesei and Neurospora crassa) further increased protein content and lowered ANFs. Solid-state fermentation of 144 h old sprouts using A. pullulans, N. crassa and T. reesei enhanced the protein content and reduced ANFs of sprouts. Sprouting canola seed for three days helped in hull removal, leading to high protein meal accompanied by low fiber and phytic acid level. Three-day seed sprouting had no effect on oil yield, but free fatty acid content was higher compared to seed oil. Co-culture fermentation of HECM under solid state process showed maximum reduction in fiber content with co-culture of A. pullulans and N. crassa while a combination of A. pullulans and T. reesei promoted the highest GLS and phytic acid reductions compared to other combinations, which indicated the advantage of coculture inoculation over monoculture in terms of ANFs reduction. Mild pretreatment of HECM using deionized water resulted in washed HECM (WHECM) with lower soluble sugars and GLS compared to untreated HECM, whereas protein and amino acid were concentrated due to the removal of soluble components. WHECM compared to HECM showed higher protein digestibility when fed to rainbow trout. Subsequent mono- and coculture fermentation of HECM and WHECM under submerged process resulted in higher protein and amino acid content and lower ANFs levels compared to uninoculated controls. The results of cellulase, endoglucanase and β-glucosidase activity indicated the crucial role of used substrates, fungi, fermentation modes (solid state/submerged stated) and inoculation methods (mono-/co-culture) on enzyme activities.