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Discovering Potential Protein, Carbohydrate, And Lipid Based Food Ingredients In A Co-Culture Of Microalgae, Chelsea Tyus Jun 2016

Discovering Potential Protein, Carbohydrate, And Lipid Based Food Ingredients In A Co-Culture Of Microalgae, Chelsea Tyus

LSU Doctoral Dissertations

Louisiana Native Co-Culture of Microalgae (Chlorella Vulgaris L. and Cyanobacteria Leptolyngbya sp.) (CCA) was studied. CCA is a viable coculture for further investigation as a food component. This research characterized the proteins, carbohydrates, and lipids present in CCA. Algae cultivation parameters were controlled and analyzed. Treatment (Trt) 1 was CCA grown in cultures exposed to average scalar irradiance (ASI) of 1041 ± 269.18 μmol m-2 s-1 PAR and trt 2 was CCA grown in cultures exposed to ASI of 430 ± 96.03 μmol m-2 s-1 PAR. The trt (irradiance exposure) had the desired response on CCA species ratio Trt …


Persistent Rna Viruses Of Common Bean (Phaseolus Vulgaris): Distribution And Interaction With The Host And Acute Plant Viruses, Surasak Khankhum Jan 2016

Persistent Rna Viruses Of Common Bean (Phaseolus Vulgaris): Distribution And Interaction With The Host And Acute Plant Viruses, Surasak Khankhum

LSU Doctoral Dissertations

Common bean (Phaseolus vulgaris) is the most important legume for direct human consumption. Common bean originated and was domesticated in the Americas but now is grown worldwide. As in the case of other crops, common bean can be infected with acute and persistent plant viruses. A modified dsRNA extraction method was developed and used in this study. The method was fast, economic, versatile, and required relatively small amounts of desiccated plant tissue. The method was successfully used to extract dsRNAs from plants infected with RNA plant viruses and to investigate the occurrence of two endornaviruses, Phaseolus vulgaris endornavirus 1 (PvEV1) …


Characterization Of Xylella Fastidiosa In Rabbiteye Blueberry, Mary Helen Ferguson Jan 2016

Characterization Of Xylella Fastidiosa In Rabbiteye Blueberry, Mary Helen Ferguson

LSU Doctoral Dissertations

Xylella fastidiosa colonizes the xylem of a wide range of plants and causes symptoms in many of them. It was of interest to determine how widespread X. fastidiosa was and what its potential impact might be in rabbiteye blueberry. X. fastidiosa was detected from two of 17 Louisiana orchards from which samples were collected. A yield study was conducted at one of these orchards, and mean yields of X. fastidiosa-positive plants were 55% and 62% less than those of X. fastidiosa-negative plants in 2013 and 2014, respectively. Average berry weight was also lower in X. fastidiosa-positive plants. However, plants that …


Evaluation Of Ammonia Volatilization And Zinc Nutrition Of Experimental Zinc Sulfate Coated Urea Fertilizers In A Drill-Seeded Delayed Flood Rice Production System, Nutifafa Adotey Jan 2016

Evaluation Of Ammonia Volatilization And Zinc Nutrition Of Experimental Zinc Sulfate Coated Urea Fertilizers In A Drill-Seeded Delayed Flood Rice Production System, Nutifafa Adotey

LSU Doctoral Dissertations

Approaches in controlling nitrogen (N) loss from preflood fertilizer applications in delayed flood rice (Oryza stiva) production in the mid-southern U.S.A. typically involves treating urea with urease inhibitors like N-(n-butyl) thiophosphoric triamide (NBPT). Limited research exists on managing N utilizing the combined effects of treating urea with a urease inhibitor and subsequently adding a physical zinc (Zn) sulfate coating. Zinc deficiency is also a major soil fertility constraint in flooded rice production. The objectives of this study were to: (i) determine the ammonia volatilization potential of experimental zinc sulfate coated urea (ZSCU) fertilizers on four rice soils in a controlled …


Integration Of Optical Remote Sensor-Based Yield Prediction And Impact Of Nitrogen Fertilization, Harvest Date, And Planting Scheme On Yield, Quality, And Biomass Chemical Composition In Energy Cane Production In Louisiana, Marilyn Sebial Dalen Jan 2016

Integration Of Optical Remote Sensor-Based Yield Prediction And Impact Of Nitrogen Fertilization, Harvest Date, And Planting Scheme On Yield, Quality, And Biomass Chemical Composition In Energy Cane Production In Louisiana, Marilyn Sebial Dalen

LSU Doctoral Dissertations

The established sugarcane industry in Louisiana is perceived as an advantage for biofuel industry because of the similarities of energy cane and sugarcane by way they are cultivated, harvested, and processed. This study was conducted at the LSU AgCenter Sugar Research Station in St. Gabriel, LA from 2013-2015 to evaluate the influence of planting scheme, N rate, and harvest date on energy cane yield, quality parameters, nutrient uptake, and biomass chemical composition. The relationship of vegetation indices (VI) with stalk, fiber yield, and N uptake of energy cane harvested at different dates was also evaluated. The experiments consisted of variety …


Identification Of Genes Associated With Resistance To Brown Rust In Sugarcane And Prevalence Of One Major Gene, Mavir Carolina Avellaneda Barbosa Jan 2016

Identification Of Genes Associated With Resistance To Brown Rust In Sugarcane And Prevalence Of One Major Gene, Mavir Carolina Avellaneda Barbosa

LSU Doctoral Dissertations

Development of resistant cultivars is the main control measure against sugarcane brown rust caused by Puccinia melanocephala. Durability is uncertain, since the pathogen possesses adaptive ability to overcome host plant resistance. A differential gene expression study utilizing suppressive subtraction hybridization was conducted to improve understanding of brown rust resistance mechanisms in sugarcane. The expression patterns of 11 unigenes representing biosynthetic pathways, defense-related genes, and signaling genes were analyzed in L 99-233, a cultivar exhibiting quantitative resistance, L 01-299, a resistant cultivar with the major resistance gene Bru1, and two susceptible cultivars, Ho 95-988 and L 09-125, at 24 h, 48 …


Molecular Genetics Of Salinity Tolerance In Rice (Oryza Sativa L.), Teresa Bermejo De Leon Jan 2016

Molecular Genetics Of Salinity Tolerance In Rice (Oryza Sativa L.), Teresa Bermejo De Leon

LSU Doctoral Dissertations

Due to the threat of salinity stress to Louisiana rice production, an effort was made to understand the molecular genetics of salinity tolerance with the overall goal of developing salt tolerant varieties. The objectives of this study were to 1) determine if salinity tolerance exist in the US rice varieties, 2) map the additive and epistatic QTLs for traits related to seedling salinity tolerance in recombinant inbred lines (RILs) using genotyping-by-sequencing (GBS)-derived SNP markers, and 3) identify and validate stable QTLs and their effects in introgression lines (ILs) of Pokkali in Bengal background. All experiments for phenotypic characterization were conducted …


Development Of Functional Markers For Resistance To Leaf Scald In Sugarcane, Andres Felipe Gutierrez Viveros Jan 2016

Development Of Functional Markers For Resistance To Leaf Scald In Sugarcane, Andres Felipe Gutierrez Viveros

LSU Doctoral Dissertations

Leaf scald, caused by Xanthomonas albilineans, is a major sugarcane disease worldwide. The disease is managed primarily with resistant cultivars obtained through classical breeding; however, the erratic symptom expression hinders the reliability and reproducibility of the selection process. The development of molecular markers associated with incompatible/compatible reaction can overcome this limitation. Suppression subtractive hybridization (SSH) and quantitative trait locus (QTL) mapping were the strategies used to find leaf scald resistance-associated genes and molecular markers in sugarcane. SSH results showed that genes involved in signal perception and transduction, and DNA binding, were highly expressed in the resistant clone LCP 85-384 compared …