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Plant Sciences

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Louisiana State University

LSU Doctoral Dissertations

Theses/Dissertations

2016

Andean

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Persistent Rna Viruses Of Common Bean (Phaseolus Vulgaris): Distribution And Interaction With The Host And Acute Plant Viruses, Surasak Khankhum Jan 2016

Persistent Rna Viruses Of Common Bean (Phaseolus Vulgaris): Distribution And Interaction With The Host And Acute Plant Viruses, Surasak Khankhum

LSU Doctoral Dissertations

Common bean (Phaseolus vulgaris) is the most important legume for direct human consumption. Common bean originated and was domesticated in the Americas but now is grown worldwide. As in the case of other crops, common bean can be infected with acute and persistent plant viruses. A modified dsRNA extraction method was developed and used in this study. The method was fast, economic, versatile, and required relatively small amounts of desiccated plant tissue. The method was successfully used to extract dsRNAs from plants infected with RNA plant viruses and to investigate the occurrence of two endornaviruses, Phaseolus vulgaris endornavirus 1 (PvEV1) …